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Method and product for detecting SARS-CoV-2 novel coronavirus IgM/IgG antibodies

A technology of coronavirus, sars-cov-2, applied in the biological field to achieve high detection sensitivity, short detection time, effective on-site testing and diagnosis

Pending Publication Date: 2021-12-03
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few kits for the detection of new coronavirus antibodies, and there is an urgent need to improve the detection rate, detection time, and ease of operation of existing technologies

Method used

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  • Method and product for detecting SARS-CoV-2 novel coronavirus IgM/IgG antibodies
  • Method and product for detecting SARS-CoV-2 novel coronavirus IgM/IgG antibodies
  • Method and product for detecting SARS-CoV-2 novel coronavirus IgM/IgG antibodies

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Embodiment 1

[0031] Material:

[0032] 1. Reagents

[0033] (1) PBS buffer (pH7.4): sterile PBS buffer, components NaCl 137mmol / L, KCl 2.7mmol / L, NaCl 2 HPO 4 10mmol / L, KH 2 PO 4 2mmol / L. Aseptically dispense 20mL into sterilized containers, store at room temperature,

[0034] (2) The buffer solution used for coating protein and antibody preparation, etc., the composition is PBS buffer solution (pH7.4), same as item (1),

[0035] (3) The washing solution used for washing after coating is, for example, PBST: PBS (pH7.4), 0.05% Tween 20,

[0036] (4) The diameter of the fluorescent microspheres can be 200nm, the excitation light wavelength is 365nm, and the emission light wavelength is 618nm.

[0037] 2. Antigens and Antibodies

[0038] The SARS-CoV-2 novel coronavirus recombinant N protein antigen used in the present invention is a recombinant protein with a GST tag at the N-terminus, which is expressed prokaryotically using a codon-optimized sequence, and purified through an affi...

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Abstract

The invention belongs to the technical field of biology, and relates to a method and product for detecting SARS-CoV-2 novel coronavirus IgM and IgG antibodies based on a fluorescence immunochromatography technology. The method provided by the invention is a one-step method and comprises the following steps that: a fluorescent microsphere labeled SARS-CoV-2 recombinant N protein antigen is loaded on a carrier; a detection carrier connected with the microsphere labeled antigen carrier is coated with a rat anti-human IgM / IgG antibody so as to be adopted as a detection line; a fluorescent microsphere labeled chicken IgY is correspondingly coated with a goat anti-chicken IgY antibody so as to be adopted as a quality control line; human plasma, serum or a whole blood sample is dropped on the fluorescent microsphere labeled antigen carrier, if the sample contains an SARS-CoV-2 novel coronavirus antibody, the detection line can be formed on the detection carrier, and meanwhile, the quality control line is positive; and if only the quality control line is displayed, and the detection line is not displayed, the result is negative. With the method and product for detecting the SARS-CoV-2 novel coronavirus IgM / IgG antibodies, the problem of clinical rapid diagnosis of SARS-CoV-2 novel coronavirus infection can be solved. The method and product have the advantages of high detection sensitivity, strong specificity, high accuracy, simplicity and rapidness in operation and capability of selecting an instrument to read and judge.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for detecting SRS-CoV-2 novel coronavirus antibodies in serum (plasma), in particular to a method for detecting SARS-CoV-2 novel coronavirus IgM and IgG antibodies based on fluorescence immunochromatography and products. Background technique [0002] The novel coronavirus pneumonia caused by the SARS-CoV-2 novel coronavirus is an acute respiratory infectious disease, characterized by fever, fatigue, and dry cough, clinical hypoxia and hypoxia, and dyspnea, and severe cases rapidly progress to acute respiratory Distress syndrome, septic shock, uncorrectable metabolic acidosis, and coagulopathy. In order to reduce the impact and harm of the new coronavirus infection on public health, scientists and technicians in the field are working on research and development including mechanism, diagnosis, drug treatment and vaccine prevention. Among them, the timely detection of patients ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558
CPCG01N33/6854G01N33/582G01N33/585G01N33/56983G01N33/558G01N2333/165G01N2469/20
Inventor 隋国栋冯萌陈军荀静娜赵望徐锦卢洪洲范列英程训佳
Owner FUDAN UNIV
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