Wild mulberry phellinus igniarius strain separation and purification method

Abstract

The invention discloses a wild mulberry phellinus igniarius strain separation and purification method, and relates to the technical field of phellinus igniarius culture, phellinus igniarius has the advantages of high growth speed and short separation period, and the specific scheme is as follows: the method comprises the following steps: S1, performing surface disinfection on phellinus igniarius sporocarp under a sterile condition; S2, cutting off fungus blocks from the phellinus igniarius sporocarp and transplanting to a plate culture medium to be cultured under the dark condition; S3, observing the growth condition of phellinus igniarius, picking bright yellow or deep yellow hyphae through an inoculating loop when the bright yellow or deep yellow hyphae are found, separating on a new culture medium by adopting a four-region plate streaking method, and continuously culturing; S4, judging when infectious microbes appear, and repeating the step S3 when the infectious microbes affect the growth of the phellinus igniarius; when the infectious microbes do not affect the growth of the phellinus igniarius, the infectious microbes are removed under the sterile condition until purified phellinus igniarius is obtained; compared with a traditional broad-spectrum culture medium, the culture medium provided by the invention is not prone to infectious microbe infection and can resist infectious microbe.

Description

technical field [0001] The invention relates to the field of Phellinus cultivating technology, more specifically, it relates to a method for separating and purifying wild Phellinus phellinus species. Background technique [0002] Phellinus japonica is a rare medicinal fungus parasitic on mulberry trees. It has high medical and health effects. Modern medical research shows that Phellinus contains active ingredients such as polysaccharides, flavonoids, and triterpenes, which can improve immunity, resist oxidation and aging, prevent and fight cancer, nourish and protect the liver, and treat prostate and gout. It is currently internationally recognized as the most efficient fungus in the field of biological cancer treatment using immunotherapy, and has become a hot spot in the research and development of pharmaceutical preparations and health care products at home and abroad. In 1968, Japanese scholars found that Phellinus can inhibit tumor rate as high as 96.7% in clinical exp...

AI Technical Summary

Problems solved by technology

[0003] The number of wild Phellinus is scarce, and the yield and quality of artificially cultivated Phellinus are not high, which cannot meet the market demand

The yield and quality of Phellinus cultivated artificially are not high, the most fundamental reason is that Phellinus species degenerates and the growth vigor is poor

The wild mulberry tree Phellinus has strong vitality and good adaptability. Therefore, the isolation and purification of the wild mulberry Phellinus as the artificially cultivated Phellinus is of great significance for subsequent scientific research and production. However, the separation and purification work is often due to the lack of suitable In the culture medium, a large number of miscellaneous bacteria appear, and the growth rate of the target strain Phellinus is slow, and the ability to resist miscellaneous bacteria is poor. It is difficult to obtain pure Phellinus colonies through one or two tests, and repeated trials are required, resulting in a separation and purification cycle that takes 3- The time of 6 months has hindered the process of large-scale and industrialization of artificial cultivation Phellinus

In order to solve the above problems, the present invention provides a preparation method of a special culture medium for the separation and purification of Phellinus spp., which solves the problems of poor vitality of Phellinus spp., easy contamination of miscellaneous bacteria, and long separation and purification period.

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