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Composite microbial agent for treating high-COD cutting fluid waste liquid and technique

A compound bacterial agent and waste liquid treatment technology, applied in microorganism-based methods, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of cutting fluid waste liquid treatment, waste of time and cost of enterprises, and reduce time cost, time-efficiency improvement

Pending Publication Date: 2021-12-07
TIANJIN RES INST FOR ADVANCED EQUIP TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few special microbial agents for the treatment of cutting fluid waste at present, so it is necessary to develop corresponding highly efficient degradation agents
On the other hand, biodegradation currently trades time efficiency for economic efficiency, and in reality, due to the lack of a method for judging the shortest biodegradation time, waste liquid will stay in the degradation tank for a long time, which will cause waste of time and cost for enterprises , so this patent also proposes a method that can evaluate the shortest degradation time of biological agents on waste liquid

Method used

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  • Composite microbial agent for treating high-COD cutting fluid waste liquid and technique
  • Composite microbial agent for treating high-COD cutting fluid waste liquid and technique
  • Composite microbial agent for treating high-COD cutting fluid waste liquid and technique

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Effect test

Embodiment 1

[0034] First, 450 g of Bacillus subtilis, 100 g of Pseudomonas aeruginosa, 50 g of Pseudomonas stutzeri, 150 g of Stenotrophomonas maltophilia, 150 g of Acinetobacter johnsonii, and 100 g of Pseudomonas putida were configured in proportion; At the same time, prepare 4.5 g of carbohydrate protective agent glucose, 0.2 g of protein protective agent serum albumin, 0.2 g of amino acid salt protective agent sodium glutamate, and 0.1 g of mixed protective agent skim milk with 5 g of distilled water in proportion to form a composite protective agent; then mix the mixed bacterial liquid and protective agent evenly, ferment at 34°C for 48 hours, vacuum freeze-dry and pulverize to make a composite bacterial agent.

[0035] The composite bacteria agent prepared by this patent is used to treat the cutting fluid waste liquid of a metal processing enterprise in Tianjin. Waste liquid COD value: 113792mg / L. Add a compound bacterial agent with a mass fraction of 1%, and use a biorespiration r...

Embodiment 2

[0037] First, 500 g of Bacillus subtilis, 150 g of Pseudomonas aeruginosa, 100 g of Pseudomonas stutzeri, 100 g of Stenotrophomonas maltophilia, 100 g of Acinetobacter johnsonii, and 50 g of Pseudomonas putida are configured in proportion; At the same time, 4.4 g of glucose protecting agent, 0.25 g of protein protecting agent serum albumin, 0.25 g of amino acid salt protecting agent sodium glutamate, and 0.1 g of mixed protecting agent skim milk were prepared in proportion with 5 g of distilled water Then, the mixed bacterial solution and protective agent were evenly mixed, fermented at 36° C. for 40 hours, then vacuum freeze-dried and pulverized to form a composite bacterial agent.

[0038] The composite bacterial agent made by this patent is used to treat the cutting fluid waste liquid of a metal processing enterprise in Shenzhen. Waste liquid COD value: 211464mg / L. Add a compound bacterial agent with a mass fraction of 1%, and use a biorespiration rate meter to perform a d...

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Abstract

The invention provides a composite microbial agent for treating high-COD cutting fluid waste liquid and a technique. The composite microbial agent mainly comprises bacillus subtilis, pseudomonas aeruginosa, pseudomonas stutzeri, stenotrophomonas maltophilia, acinetobacter johnsonii and pseudomonas putida. A judgment method of the microbial agent for the shortest degradation time of the cutting fluid waste liquid comprises the steps of measuring the total biological oxygen consumption through a biological respiratory rate meter, and determining the shortest degradation time through an oxygen consumption curve. The composite microbial agent is good in degradation effect and low in operation cost. According to the judgment method of the microbial agent for the shortest degradation time of the cutting fluid waste liquid, the degradation condition of the waste liquid at any moment can be quantitatively obtained, the shortest degradation time of the microbial agent for the waste liquid is obtained, the waste liquid treatment time is further shortened on the basis that the degradation effect is guaranteed, and the time efficiency of the degradation process is improved.

Description

technical field [0001] The invention belongs to the field of metalworking fluid waste treatment, and in particular relates to a composite bacterial agent and a process method for treating high COD cutting fluid waste. Background technique [0002] At present, the demand for metalworking fluids in my country is increasing year by year, and the annual consumption is about 4.0-5.0×10 5 t. Among them, cutting fluid accounts for about 35-40% of the total consumption of metalworking fluid, and it shows an increasing trend. With the extensive use of cutting fluid, the problem of waste fluid treatment needs to be solved urgently. Cutting fluid waste liquid is currently one of the most difficult industrial waste liquids in the machining industry. It usually contains mineral oil, animal and vegetable oils, surfactants, anti-rust agents, etc. Most of the COD values ​​​​are as high as hundreds of thousands, and direct discharge is harmful to the environment. Great harm to the environ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/385C12R1/38C12R1/01C12R1/40
CPCC12N1/20C02F3/34
Inventor 戴媛静程慧杰赵朋飞李小磊张晨辉雒建斌
Owner TIANJIN RES INST FOR ADVANCED EQUIP TSINGHUA UNIV
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