Construction method of glucose biosensor assembled based on glutathione

A biosensor and glutathione technology, applied in the field of biosensors, can solve the problems of undiscovered graphene oxide and glutathione biosensors, etc., and achieve good direct electrochemical behavior, good catalytic effect, and good selectivity Effect

Pending Publication Date: 2021-12-07
齐鲁医药学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there is no report on the prep...

Method used

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  • Construction method of glucose biosensor assembled based on glutathione
  • Construction method of glucose biosensor assembled based on glutathione
  • Construction method of glucose biosensor assembled based on glutathione

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Preparation of glutathione-modified gold electrodes

[0049] Gold electrodes with a diameter of 1.0 mm were coated with 0.05 μm Al 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, cleaned with an ultrasonic cleaner for 10 minutes, taken out and dried naturally, and ultrasonically cleaned with 75% alcohol for 10 minutes. Using cyclic voltammetry (CV) at 2.5×10 -3 Scan in mol / L potassium ferricyanide solution (containing 5.0mol / L KCl) until it is stable, and the potential difference is within 100mV.

[0050] Weigh 0.7683g glutathione and dissolve it in a phosphate buffer solution with a pH value of 7.00 to make 50mL glutathione solution with a concentration of 0.05mol / L, suspend and soak the ground gold electrode in 0.05mol / L Soak in glutathione solution for 24 hours at 4°C, and store in a refrigerator at 4°C for later use.

[0051] (2) Preparation of carboxylated graphene

[0052] Assemble a 250ml reaction bottle in an ice-water bat...

Embodiment 2

[0059] (1) Preparation of glutathione-modified gold electrodes

[0060] Gold electrodes with a diameter of 1.0 mm were coated with 0.06 μm Al 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, cleaned with an ultrasonic cleaner for 10 minutes, taken out and dried naturally, and ultrasonically cleaned with 75% alcohol for 10 minutes. Using cyclic voltammetry (CV) at 3.0×10 -3 Scan in mol / L potassium ferricyanide solution (containing 5.5mol / L KCl) until it is stable, and the potential difference is within 100mV.

[0061] Weigh 0.9220g glutathione and dissolve it in a phosphate buffer solution with a pH value of 7.00 to make 50mL glutathione solution with a concentration of 0.06mol / L, suspend and soak the ground gold electrode in 0.06mol / L Soak in glutathione solution for 20 hours at 4°C, and store in a refrigerator at 4°C for later use.

[0062] (2) Preparation of carboxylated graphene

[0063] Assemble a 250ml reaction bottle in an ice-water bat...

Embodiment 3

[0070] (1) Preparation of glutathione-modified gold electrodes

[0071] Gold electrodes with a diameter of 1.0 mm were coated with 0.05 μm Al 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, cleaned with an ultrasonic cleaner for 10 minutes, taken out and dried naturally, and ultrasonically cleaned with 75% alcohol for 10 minutes. Using cyclic voltammetry (CV) at 2.8×10 -3 Scan in mol / L potassium ferricyanide solution (containing 5.2mol / L KCl) until it is stable, and the potential difference is within 100mV.

[0072] Weigh 0.6146g glutathione and dissolve it in a phosphate buffer solution with a pH value of 7.00 to make 50mL glutathione solution with a concentration of 0.04mol / L, suspend and soak the ground gold electrode in 0.04mol / L Soak in glutathione solution for 24 hours at 5°C, and store in a refrigerator at 4°C for later use.

[0073] (2) Preparation of carboxylated graphene

[0074] Assemble a 250ml reaction bottle in an ice-water bat...

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Abstract

The invention belongs to the technical field of biosensors, and particularly relates to a construction method of a glucose biosensor assembled based on glutathione. The method comprises the following steps: by taking a glutathione modified gold electrode as a carrier, activating carboxylated graphene, loading the activated carboxylated graphene on the surface of the glutathione modified gold electrode to obtain a carboxylated graphene/glutathione modified gold electrode, soaking the carboxylated graphene/glutathione modified gold electrode in a cross-linking agent, and airing to obtain the glucose biosensor assembled based on glutathione. According to the invention, the glutathione-carboxylated graphene self-assembled glucose biosensor is successfully assembled. Glutathione and carboxylated graphene are used for immobilizing glucose oxidase, and the glucose biosensor with high sensitivity, good selectivity, good reproducibility and good stability is constructed.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to a method for constructing a glucose biosensor based on glutathione assembly. Background technique [0002] Graphene (Graphene, GN) is a new type of two-dimensional carbon material, known as the "mother of carbon materials", which has excellent electrical conductivity, high carrier mobility (up to 15000cm 2 .v -1 .s -1 ), electrocatalytic performance, high specific surface area, good mechanical stability and other properties, it is an ideal electrode material for the preparation of biosensors. GN effectively improves the sensitivity and response signal of biosensors and facilitates the transport of electrons during the electrochemical process. The high specific surface area of ​​GN can increase the loading capacity of biological reagents, thus improving the sensitivity and other properties of the sensor. In addition, GN can also maintain the activity of loaded biological re...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/49
CPCG01N27/3272G01N27/49
Inventor 高先娟汲霞杜青青任云凤刘宁
Owner 齐鲁医药学院
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