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Marker and detection kit for detecting bladder cancer

A detection kit and bladder cancer technology, applied in the field of biomedical detection, can solve problems such as difficulty in specifically diagnosing bladder cancer, and achieve the effects of high sensitivity, high sensitivity and specificity, and high sensitivity

Pending Publication Date: 2021-12-07
山东冠科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a single tumor marker is difficult to specifically diagnose bladder cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Preparation of antibody-coated magnetic particles

[0047] 1.1 Preparation of magnetic particles coated with mouse anti-NMP22 antibody

[0048] (1) Take 200 μL of magnetic bead solution (50 mg / mL), wash twice with 0.01M PBS buffer (p H=7.4), and finally resuspend the magnetic beads with 1 mL of PBS buffer;

[0049] (2) Add 100 μL of EDC solution to the magnetic bead solution obtained in step (1), vortex and mix well, then shake and react at room temperature for 2 hours; the room temperature is 15-40°C, which is 25°C in this example; EDC solution : 0.01M MES ((2-(N-morpholino)ethanesulfonic acid)) (PH=6.0) solution to prepare a 5mg / ml EDC solution (ready for immediate use), protected from light for later use;

[0050] (3) Discard the supernatant, wash 3 times with 0.01M PBS buffer (p H=7.4); resuspend the magnetic beads with 1mL PBS buffer;

[0051] (4) Add 50-800 μg of purified and concentrated mouse anti-NMP22 monoclonal antibody (400 μg is added in this example),...

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Abstract

The invention belongs to the technical field of biomedical detection, and particularly relates to a marker and a detection kit for detecting bladder cancer. The marker provided by the invention comprises a nuclear matrix protein 22 (NMP22), a carcino-embryonic antigen (CEA), a bladder tumor antigen (BTA) and a cytokeratin 19 fragment (CYFRA21-1). According to the detection kit, acridinium ester is adopted for direct chemiluminescence, an antibody (antigen) is directly labeled by the acridinium ester, after immune reaction with a corresponding antigen (antibody) of a sample to be detected, a coated antibody-antigen to be detected-acridinium ester labeled antibody compound is formed, excitation liquid is added, and decomposition and luminescence can be realized without a catalyst. A combination of multiple tumor markers is established, the detection specificity and sensitivity of bladder cancer are improved through combined detection of multiple indexes, when urine of a bladder cancer patient is detected, the sensitivity and specificity are high, and early screening, treatment effect and postoperative recurrence of the bladder cancer patient can be dynamically monitored.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a marker and a detection kit for bladder cancer detection. Background technique [0002] Tumor is a kind of disease that seriously threatens human health and has high morbidity and mortality. Bladder cancer is the most common malignant tumor of the genitourinary system, with hidden early symptoms, and most patients are in the middle and late stages when they seek treatment. The incidence rate ranks first among malignant tumors in the urinary system, and it has a very high recurrence rate and clinical complexity. Among malignant tumors, bladder cancer ranks sixth in incidence and fourth in mortality. [0003] At present, the diagnosis of bladder tumors is mainly based on urine testing, imaging (KUB, IVP, CT, MRI, ultrasound, etc.) examinations, and cystoscopy. The initial diagnosis of bladder tumors is mainly superficial tumors. Imaging examinations are...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/577G01N33/543G01N21/76
CPCG01N33/57407G01N33/57473G01N33/57488G01N33/577G01N33/54326G01N21/76G01N2333/4742G01N2333/47
Inventor 卢英
Owner 山东冠科生物技术有限公司
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