Detection card for identifying, diagnosing and detecting avian influenza virus infection and vaccine immunity and preparation method thereof
An avian influenza virus and differential diagnosis technology, which is applied in the field of avian influenza virus infection and vaccine immunization differential diagnosis test cards and preparation, can solve the problems of unstable product quality, poor uniformity, uneven particles, etc. Equipment and instruments, the effect of high sensitivity
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Embodiment 1
[0054] Example 1: See Figure 1-2 , the test card for differential diagnosis of avian influenza virus infection and vaccine immunity, comprising a shell 8 and a supporting sample diluent, the shell 8 is equipped with a test strip.
[0055] The test strip includes a PVC bottom plate 2, on which a sample pad 1, a marking pad 3, a coating film 6 and a water-absorbing pad 7 are pasted in sequence from one side of the PVC bottom plate 2 to the other.
[0056] The coating membrane 6 is a nitrocellulose membrane.
[0057] In this embodiment, the coating membrane 6 is a nitrocellulose membrane of model CN140 produced by Sartorius Company.
[0058] The coating film 6 is coated with a detection line 4 and a quality control line 5, the detection line 4 is coated at a position close to the marking pad 3, and the quality control line 5 is coated at a position close to the water-absorbing pad 7 .
[0059] The detection line 4 is coated with purified avian influenza NS1 protein, and the q...
Embodiment 2
[0109] Example 2: In the above-mentioned Example 1, the determination of the optimal protein labeling pH value: in the protein labeling process of latex microspheres, MES with pH values of 7.0, 7.5 and 8.0 were used for labeling respectively, and different pH values were compared. The impact of the specificity and sensitivity of the differential diagnosis test card for avian influenza virus infection and vaccine immunity, so as to determine the pH value of the optimal protein marker, the experimental structure is as follows and Figure 8 shown.
[0110] The following table shows the experimental effect of different marked pH values:
[0111]
[0112] In the above table, "+" means positive; "-" means negative.
[0113] According to the above table and Figure 8 It can be seen that when the pH value is 7.5, the sensitivity of the detection line of the differential diagnosis test card for avian influenza virus infection and vaccine immunity is better, and the color devel...
Embodiment 3
[0114] Embodiment 3: in above-mentioned embodiment 1, the selection of optimal protein labeling amount: in the protein labeling process of latex microsphere, adopt respectively marker protein and latex microsphere mass ratio to be 1:15,1:10,1: 5 mouse anti-chicken IgG monoclonal antibody for labeling, the experimental structure is shown in the following table and Figure 9 shown.
[0115] The following table shows the experimental effect of different protein labeling amounts:
[0116]
[0117] In the above table, "+" means positive; "-" means negative.
[0118] According to the above table and Figure 9 It can be seen that when the mass ratio of labeled protein to latex microspheres is 1:15, the sensitivity is poor, and the color of the detection line is weak; when 1:10, the sensitivity and specificity are good, and when 1:5, the avian influenza virus The color rendering effect of the differential diagnosis test card for infection and vaccine immunity did not change sign...
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