Detection card for identifying, diagnosing and detecting avian influenza virus infection and vaccine immunity and preparation method thereof

An avian influenza virus and differential diagnosis technology, which is applied in the field of avian influenza virus infection and vaccine immunization differential diagnosis test cards and preparation, can solve the problems of unstable product quality, poor uniformity, uneven particles, etc. Equipment and instruments, the effect of high sensitivity

Inactive Publication Date: 2021-12-07
SHANDONG VOCATIONAL ANIMAL SCI & VETERINARY COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The main technical problem to be solved by the present invention is to provide a differential diagnosis test card for avian influenza virus infection and vaccine immunity and its preparation method. During the preparation of colloidal gold test strips, the firing of colloidal gold is prone to uneven particles, resulting in unstable product quality and poor uniformity.

Method used

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  • Detection card for identifying, diagnosing and detecting avian influenza virus infection and vaccine immunity and preparation method thereof
  • Detection card for identifying, diagnosing and detecting avian influenza virus infection and vaccine immunity and preparation method thereof
  • Detection card for identifying, diagnosing and detecting avian influenza virus infection and vaccine immunity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: See Figure 1-2 , the test card for differential diagnosis of avian influenza virus infection and vaccine immunity, comprising a shell 8 and a supporting sample diluent, the shell 8 is equipped with a test strip.

[0055] The test strip includes a PVC bottom plate 2, on which a sample pad 1, a marking pad 3, a coating film 6 and a water-absorbing pad 7 are pasted in sequence from one side of the PVC bottom plate 2 to the other.

[0056] The coating membrane 6 is a nitrocellulose membrane.

[0057] In this embodiment, the coating membrane 6 is a nitrocellulose membrane of model CN140 produced by Sartorius Company.

[0058] The coating film 6 is coated with a detection line 4 and a quality control line 5, the detection line 4 is coated at a position close to the marking pad 3, and the quality control line 5 is coated at a position close to the water-absorbing pad 7 .

[0059] The detection line 4 is coated with purified avian influenza NS1 protein, and the q...

Embodiment 2

[0109] Example 2: In the above-mentioned Example 1, the determination of the optimal protein labeling pH value: in the protein labeling process of latex microspheres, MES with pH values ​​of 7.0, 7.5 and 8.0 were used for labeling respectively, and different pH values ​​were compared. The impact of the specificity and sensitivity of the differential diagnosis test card for avian influenza virus infection and vaccine immunity, so as to determine the pH value of the optimal protein marker, the experimental structure is as follows and Figure 8 shown.

[0110] The following table shows the experimental effect of different marked pH values:

[0111]

[0112] In the above table, "+" means positive; "-" means negative.

[0113] According to the above table and Figure 8 It can be seen that when the pH value is 7.5, the sensitivity of the detection line of the differential diagnosis test card for avian influenza virus infection and vaccine immunity is better, and the color devel...

Embodiment 3

[0114] Embodiment 3: in above-mentioned embodiment 1, the selection of optimal protein labeling amount: in the protein labeling process of latex microsphere, adopt respectively marker protein and latex microsphere mass ratio to be 1:15,1:10,1: 5 mouse anti-chicken IgG monoclonal antibody for labeling, the experimental structure is shown in the following table and Figure 9 shown.

[0115] The following table shows the experimental effect of different protein labeling amounts:

[0116]

[0117] In the above table, "+" means positive; "-" means negative.

[0118] According to the above table and Figure 9 It can be seen that when the mass ratio of labeled protein to latex microspheres is 1:15, the sensitivity is poor, and the color of the detection line is weak; when 1:10, the sensitivity and specificity are good, and when 1:5, the avian influenza virus The color rendering effect of the differential diagnosis test card for infection and vaccine immunity did not change sign...

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Abstract

The invention belongs to the technical field of biological detection.The invention discloses an avian influenza virus infection and vaccine immunity differential diagnosis detection card and a preparation method. The avian influenza virus infection and vaccine immunity differential diagnosis detection card comprises a shell and a sample diluent used in cooperation with the shell, and a test strip is assembled in the shell and comprises a PVC bottom plate; wherein a sample pad, a marking pad, a coating film and a water absorption pad are pasted on the PVC bottom plate, and the kit is characterized in that the marking pad is a glass cellulose film and is coated with a coupling marker of a mouse anti-chicken IgG monoclonal antibody and latex microspheres; the coating membrane is a nitrocellulose membrane, and a detection line coated with purified avian influenza NS1 protein and a quality control line coated with purified recombinant streptococcus G protein are coated on the coating membrane. The kit can identify and diagnose antibodies generated by avian influenza virus infection and vaccine immunization, has good specificity and convenience, and can well identify avian influenza infected chicken flocks.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and specifically relates to a detection card for differential diagnosis of avian influenza virus infection and vaccine immunity and a preparation method thereof. Background technique [0002] Avian influenza (Avian influenza, AI) is a highly contagious disease of poultry caused by influenza virus A of the Orthomyxoviridae family. There are 16 HA subtypes and 10 NA subtypes. The Bureau of Veterinary Epidemiology designated it as a Class A infectious disease, which is one of the devastating diseases in the poultry industry. The disease not only caused huge economic losses to the global poultry industry, but also could infect humans, causing great harm to public health security. threat. [0003] At present, one of the methods to effectively control avian influenza is vaccination. An index to effectively evaluate the immune effect is to detect the antibody level after immunization, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/543
CPCG01N33/577G01N33/56983G01N33/558G01N33/54313G01N2333/11G01N2469/20
Inventor 肖发沂齐艳君王小伟朱绍辉朱光亓鹏柏程昊
Owner SHANDONG VOCATIONAL ANIMAL SCI & VETERINARY COLLEGE
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