Melanin separation and extraction method based on ionic liquid

A technology of ionic liquid and extraction method, which is applied in the field of waste hair recycling, can solve the problems of long pretreatment time, high price of protease, low yield of melanin recovery, etc., and achieve the effect of easy separation

Active Publication Date: 2021-12-14
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The invention solves the technical problems of low melanin recovery yield, complex operation, expensive protease and long pretreatment time in the prior art.

Method used

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  • Melanin separation and extraction method based on ionic liquid
  • Melanin separation and extraction method based on ionic liquid
  • Melanin separation and extraction method based on ionic liquid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] figure 1 It is the technological process flow of the targeted extraction of melanin based on ionic liquid in the present invention.

[0031] Step 1: A mixture of 0.2 g of washed discarded human hair (hair) and 4 g of 1-butyl-3-methylimidazolium chloride (ionic liquid) was heated in a beaker at 130° C. until the discarded human hair was completely dissolved. 0.2 mL of dilute sulfuric acid (50 wt %) (depolymerization agent) was added to the mixture, and the mixture was heated at 120° C. for 1.2 hours.

[0032] Step 2: Add 20mL of aqueous solution to the mixture, shake the mixture vigorously for 2min, and then use a centrifuge to centrifuge (5000rpm, 5min). After centrifugation, the precipitate is a melanin product with a yield of 6.02%. g / ml K 3 PO 4 (Phase separation agent) The upper phase and the lower phase are separated. The upper layer solution was rich in 1-butyl-3-methylimidazolium chloride salt, was collected, and was directly recycled to step 1 after 5 hours ...

Embodiment 2

[0035] Step 1: A mixture of 1 g of washed discarded brown wool (hair) and 20 g of 1-butyl-3-methylimidazolium chloride (ionic liquid) was heated in a beaker at 130° C. until the discarded human hair was completely dissolved. 1 mL of dilute sulfuric acid (50 wt %) (depolymerization agent) was added to the mixture, and the mixture was heated at 120° C. for 2.2 hours.

[0036] Step 2: Add 100mL of aqueous solution to the mixture, shake the mixture vigorously for 6min, and then use a centrifuge to centrifuge (5000rpm, 10min). After centrifugation, the precipitate is a melanin product with a yield of 7.24%. g / ml K 3 PO 4 (Phase-separating agent) The upper phase and the lower phase are separated. The upper layer solution was rich in 1-butyl-3-methylimidazolium chloride salt, was collected, and was directly recycled to step 1 after 5 hours of vacuum distillation. The lower layer solution is rich in compound amino acids and phase-splitting agent, and the lower phase rich in compound ...

Embodiment 3

[0039] Carry out SEM test on the basis of embodiment 1, test result sees figure 2 .

[0040] Test description: Usually, it is possible to directly observe whether the surface morphology of melanin remains in the original nano-particle shape through SEM testing, so as to judge whether the natural morphology of melanin is destroyed. It is generally considered that granular melanin that maintains its natural shape is an excellent melanin product.

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Abstract

The invention relates to a melanin separation and extraction method based on ionic liquid, and belongs to the technical field of waste hair recycling. The method comprises the following steps: depolymerizing hair by using an ionic liquid and a depolymerizing agent, separating amino acid, the ionic liquid and melanin in one step by using an aqueous two-phase system, extracting the amino acid in a phase-splitting agent, and recovering the ionic liquid and the phase-splitting agent. Actual operation steps are simple, high-purity melanin can be efficiently separated, melanin particles keep the original appearance, and follow-up high-end application is facilitated; and main reagents can be recovered and reutilized, so that the production cost can be greatly reduced. The method has the advantages of high efficiency, no emission of three wastes, excellent economic feasibility and the like.

Description

technical field [0001] The invention belongs to the technical field of waste hair recycling, and more specifically relates to a method for separating and extracting melanin based on ionic liquid. Background technique [0002] Waste hair is solid waste produced by humans and animals, with an annual global output of more than one million tons. Discarded hair contains a large amount of valuable component substances such as keratin and melanin. Keratin is easily hydrolyzed to produce amino acids, which can be used in many industries. Melanin is also considered as a useful biopigment and has been applied in the fields of surface modification, cell interface, biosensing application and nanomedicine. However, there are still many shortcomings in the resource recovery process of hair today: in industrialized process methods, strong acid hydrolysis is usually used to recover amino acids, which uses strong acid to hydrolyze hair under high temperature conditions, which will produce ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 陈朱琦罗放赵钦地姜旺王训
Owner HUAZHONG UNIV OF SCI & TECH
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