Ni3 (HITP) 2 field effect transistor biosensor and preparation method thereof
A technology of field-effect transistors and biosensors, applied in the field of biosensors, to achieve the effects of good conductivity, improved detection sensitivity, and porous films
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Embodiment 1
[0044] A kind of Ni 3 (HITP) 2 A field effect transistor biosensor and a preparation method thereof, comprising the following steps:
[0045] Step 1, based on Ni 3 (HITP) 2 Field-effect transistor, using polydimethylsiloxane (PDMS) to prepare a cylindrical liquid tank in the channel region, its cross-sectional inner diameter is 3 mm, and the height is 1 mm; after curing at 25 ° C, it forms a PDMS liquid for storing the solution to be tested. groove.
[0046] Step 2. Take 15 μL of glutaraldehyde solution with a mass concentration of 0.1% and add it dropwise to the device liquid tank for incubation. The incubation temperature is 25° C. and the incubation time is 1 hour; / mL of the new crown antigen molecule solution (SRAS-COV-2N protein, 0588-V07E, Sino Biological Inc.) was added dropwise to the device liquid tank for incubation, the incubation temperature was about 0°C, and the incubation time was 8h; after the incubation was completed, Ni 3 (HITP) 2 Field-effect transist...
Embodiment 2
[0052] A kind of Ni 3 (HITP) 2A field effect transistor biosensor and a preparation method thereof, comprising the following steps:
[0053] Step 1, based on Ni 3 (HITP) 2 Field-effect transistor, using polydimethylsiloxane (PDMS) to prepare a cylindrical liquid tank in the channel area, the inner diameter of the cross section is 4mm, and the height is 1.5mm; after curing at 25°C, a PDMS liquid tank for storing the solution to be tested is formed .
[0054] Step 2. Take 25 μL of glutaraldehyde solution with a mass concentration of 0.5% and add it dropwise to the device liquid tank for incubation. The incubation temperature is 25°C and the incubation time is 2 hours. / mL of the new crown antigen molecule solution was added dropwise to the device liquid tank for incubation, the incubation temperature was about 0°C, and the incubation time was 12 hours; after the incubation was completed, Ni 3 (HITP) 2 Field Effect Transistor Biosensors.
[0055] Step 3. Take out the prepa...
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