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Ni3 (HITP) 2 field effect transistor biosensor and preparation method thereof

A technology of field-effect transistors and biosensors, applied in the field of biosensors, to achieve the effects of good conductivity, improved detection sensitivity, and porous films

Active Publication Date: 2021-12-14
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing technology, a MOF-based field effect tube biosensor has not been developed to realize the convenient and sensitive detection of the new coronavirus antibody molecules.

Method used

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  • Ni3 (HITP) 2 field effect transistor biosensor and preparation method thereof
  • Ni3 (HITP) 2 field effect transistor biosensor and preparation method thereof
  • Ni3 (HITP) 2 field effect transistor biosensor and preparation method thereof

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Effect test

Embodiment 1

[0044] A kind of Ni 3 (HITP) 2 A field effect transistor biosensor and a preparation method thereof, comprising the following steps:

[0045] Step 1, based on Ni 3 (HITP) 2 Field-effect transistor, using polydimethylsiloxane (PDMS) to prepare a cylindrical liquid tank in the channel region, its cross-sectional inner diameter is 3 mm, and the height is 1 mm; after curing at 25 ° C, it forms a PDMS liquid for storing the solution to be tested. groove.

[0046] Step 2. Take 15 μL of glutaraldehyde solution with a mass concentration of 0.1% and add it dropwise to the device liquid tank for incubation. The incubation temperature is 25° C. and the incubation time is 1 hour; / mL of the new crown antigen molecule solution (SRAS-COV-2N protein, 0588-V07E, Sino Biological Inc.) was added dropwise to the device liquid tank for incubation, the incubation temperature was about 0°C, and the incubation time was 8h; after the incubation was completed, Ni 3 (HITP) 2 Field-effect transist...

Embodiment 2

[0052] A kind of Ni 3 (HITP) 2A field effect transistor biosensor and a preparation method thereof, comprising the following steps:

[0053] Step 1, based on Ni 3 (HITP) 2 Field-effect transistor, using polydimethylsiloxane (PDMS) to prepare a cylindrical liquid tank in the channel area, the inner diameter of the cross section is 4mm, and the height is 1.5mm; after curing at 25°C, a PDMS liquid tank for storing the solution to be tested is formed .

[0054] Step 2. Take 25 μL of glutaraldehyde solution with a mass concentration of 0.5% and add it dropwise to the device liquid tank for incubation. The incubation temperature is 25°C and the incubation time is 2 hours. / mL of the new crown antigen molecule solution was added dropwise to the device liquid tank for incubation, the incubation temperature was about 0°C, and the incubation time was 12 hours; after the incubation was completed, Ni 3 (HITP) 2 Field Effect Transistor Biosensors.

[0055] Step 3. Take out the prepa...

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Abstract

The invention belongs to the field of biosensors, and discloses a Ni3 (HITP) 2 field effect transistor biosensor and a preparation method thereof. The preparation method comprises the steps that: based on a Ni3 (HITP) 2 field effect transistor, a solution tank is prepared, a solution in the solution tank can make contact with a channel region of the transistor, and the surface of a channel region material is modified with a glutaraldehyde cross-linking agent; and new coronavirus antigen protein probe molecules are fixed to the surface of the channel material through the glutaraldehyde cross-linking agent to obtain the Ni3 (HITP) 2 field effect transistor biosensor. The liquid tank can contain liquid to be detected, and the novel coronavirus antibody molecules in the liquid to be detected are detected by utilizing the electrical property change of the biosensor. The detailed structure of the biosensor is designed and improved; the obtained biosensor can rapidly and sensitively detect the novel coronavirus antibody molecules (especially low-concentration novel coronavirus antibody molecules), and the detection limit is as low as 10 pg / mL.

Description

technical field [0001] The invention belongs to the field of biosensors, more specifically, relates to a Ni 3 (HITP) 2 A field effect transistor biosensor and a preparation method thereof, the biosensor can be applied to the detection of novel coronavirus. Background technique [0002] Metal-organic framework (MOF) is a kind of organic-inorganic hybrid material with adjustable structure formed by self-assembly of organic ligands and metal centers. It has the advantages of adjustable structure, large specific surface area, and many active adsorption sites. Widely used in gas storage and separation, catalysis and sensing and other fields. Most of the traditional MOF materials have defects of poor conductivity, which limits their application in electronic devices. However, with the successful preparation of conductive MOF materials, electronic devices based on two-dimensional conductive MOF materials have become a research hotspot. [0003] Novel coronavirus pneumonia is a n...

Claims

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Application Information

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IPC IPC(8): G01N27/414G01N33/531G01N33/569
CPCG01N27/4145G01N33/531G01N33/56983G01N2469/20G01N2333/165Y02A50/30
Inventor 段国韬沈树阳谭彭伟唐宇枢
Owner HUAZHONG UNIV OF SCI & TECH
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