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Method for detecting and evaluating quality of adult tamarixia radiata

A technology of wasps and wasps, which is applied in the field of quality detection and evaluation of adult wasps of bright bellies, can solve problems such as differences in parasitism rates, achieve the effects of controlling population numbers, promoting commercialization, and ensuring the quality and safety of agricultural products and the safety of the ecological environment

Active Publication Date: 2021-12-17
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]However, the parasitism rate of the wasps produced in different regions is significantly different

Method used

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  • Method for detecting and evaluating quality of adult tamarixia radiata
  • Method for detecting and evaluating quality of adult tamarixia radiata
  • Method for detecting and evaluating quality of adult tamarixia radiata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 Identification of species morphological characteristics

[0020] Morphological identification should be carried out first to evaluate the product quality of B. japonica, so as to ensure that the morphological characteristics of the species are consistent with the detection results. In this paper, 5 bottles of B. japonica were randomly selected, and 10 of them (5 females and 5 males) were selected from each bottle for species identification. The identification results are as follows:

[0021] (1) female bee ( figure 1 ):

[0022] Body length 0.92 ~ 1.04mm, most of them are dark brown, compound eyes and monocular reddish brown, head wider than long, 2 teeth and 1 pick on the upper jaw, 1 segment of mandibular palp and lower lip palp. Antennae 8-segmented (antennary type 1, 1, 3, 3), with 1 small annular segment; the length of the stalk is 3.7 times the width; the pedicel is equal to or slightly longer than the first cord; the length of the first to third c...

Embodiment 2

[0027] Example 2 Molecular Characterization of Species

[0028] The conserved region of cytochrome oxidase subunit I (COI) and internal transcribed spacer 1, 2 (internal transcribed spacer, ITS-1, ITS-2) were used for molecular identification of species.

[0029] Randomly select 5 vials of Bright-bellied Glazed Bee, and extract 1 head from each vial for total DNA extraction. The kit uses Tiangen Bio-Blood / Cell / Tissue Genomic DNA Extraction Kit (DP304), and the experimental steps strictly follow the kit instructions. The upstream and downstream primers are listed in Table 1.

[0030] Table 1

[0031]

[0032] Use 50 μL system for PCR reaction, 2 μL DNA template, 0.5 μM each primer (Table 1), 25 μL Mix (2X SanTaq PCRMix master mix, containing blue dye, Sangon Biotech B532061), supplemented with sterile water. Reaction conditions: pre-denaturation at 94°C (5min) → denaturation at 94°C (30s) → annealing at 50-60°C (30s) → extension at 72°C (30-60s) → extension at 72°C (10min)...

Embodiment 3

[0036] Example 3 Female bee body color and disability detection

[0037] Use a stereo microscope to observe whether the body color of the female bright-bellied wasps is dim, and whether there are residual wings and limbs. Randomly select 10 bottles of bright-bellied wasps, every two bottles are 1 sample, take 200 female bright-bellied wasps for each sample, record the number of female bees with dark body color and residual wings and limbs, and count the dark body color and the ratio of the number of individuals with residual limbs and residual wings to the total number (body color dim rate and residual limb residual wing rate), a total of 5 tests were performed, and the test results are shown in Table 3.

[0038] table 3

[0039] Test items(%) 1 2 3 4 5 average body color dimming rate 1.0 1.5 0.5 1.0 1.5 1.1±0.2 Remnant Limb Remnant Wing Rate 0.2 0.4 0.4 0.4 0.4 0.36±0.4

[0040]It can be seen from Table 3 that in the 5 times of app...

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Abstract

The invention discloses a method for detecting and evaluating the quality of adult tamarixia radiata, and belongs to the field of biological prevention and control of agricultural pests. According to the method, seven quality indexes including species morphological characteristics, species molecular characteristics, appearance, product quantity, female bee rate, parasitism rate and storage death rate of tamarixia radiata at different temperatures are detected, and then evaluation is carried out according to established quality standards. According to the method, the commodity quality of the adult tamarixia radiata can be improved, the product quality management is enhanced, and the quality safety of agricultural products and the ecological environment safety are guaranteed; and by using the detection method to screen the qualified tamarixia radiata and release the tamarixia radiata to the field, the population number of diaphorina citri can be effectively controlled, so that commercialization of natural enemy products of the tamarixia radiata is promoted.

Description

technical field [0001] The invention belongs to the field of biological control of agricultural pests, and in particular relates to a method for detecting and evaluating the quality of adult beetle wasps. Background technique [0002] Tamarixia radiata is the dominant parasitoid of the citrus psyllid (Diaphorina citri Kuwayama), parasitizing the 4-5 instar nymphs of the citrus psyllid. A single wasp can kill up to 500 citrus psyllid nymphs by feeding and parasitizing. In 1998, the U.S. state of Florida introduced the bright-bellied bee for release. By 2006, the bee had spread to all the orange groves in Florida. , China Taiwan, the Philippines and Mauritius in Africa and other regions have been successfully introduced; they are distributed in Brazil, Argentina, Venezuela, Mexico and other regions. At present, the bright belly glaze has been produced in factories in Brazil, Mexico, California, Texas and Florida in the United States. In China, the Institute of Zoology of the...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01M99/00
CPCA01K67/033A01M99/00
Inventor 陈玲玲王朝红毛润乾黄承志高晶修宝林叶凤娴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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