Method for culturing microalgae by combining micro-polluted source water with percolate

Abstract

The invention relates to a method for culturing microalgae by combining micro-polluted source water with percolate. The method comprises the following steps of S1, respectively passing the percolate and the micro-polluted source water through rapid filter paper to remove large particulate matters so as to form percolation filtrate and micro-polluted source water filtrate; S2, inoculating 0.1% of a microalgae concentrated solution into each 1.5 L of the micro-polluted source water filtrate to form a culture solution, and culturing for 6 days until the microalgae grow to the initial stage of a mature stage; S3, on the seventh day to the twelfth day, adding percolation filtrate accounting for 1% of the volume of the culture solution into the culture solution every day, and domesticating the algae species to adapt to the continuously-increased percolation concentration; S4, on the 13th-17th day, adding the percolation filtrate accounting for 1% of the volume of the culture solution and the fresh micro-polluted source water filtrate accounting for 5% of the volume of the culture solution into the culture solution every day, discharging 5% of the culture solution, treating microalgae discharged liquid, and harvesting microalgae biomass; and S5, starting from the 18th day, adding the percolation filtrate accounting for 2% of the volume of the culture solution and the fresh micro-polluted source water filtrate accounting for 10% of the volume of the culture solution into the microalgae culture solution every day, and discharging 10% of the culture solution.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation and pollutant removal, in particular to a method for cultivating microalgae by using slightly polluted water source water combined with leachate. Background technique [0002] With the acceleration of urbanization and the improvement of residents' living standards, part of the source water is affected by the discharge of industrial wastewater and domestic sewage, and some indicators such as ammonia nitrogen, total phosphorus, chroma, organic matter, heavy metals and other indicators exceed the environmental quality standards for surface water, forming Slightly polluted source water. On the water source of lakes and reservoirs, it is manifested as eutrophication of reservoirs and lakes, and in a certain period of time, algae breed, causing water quality to deteriorate and odor to increase significantly. From another point of view, microalgae need a large amount of nutrients such as ...

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Problems solved by technology

[0003] In recent years, scholars at home and abroad have used the characteristics of chlorella to grow rapidly under extreme conditions to carry out microalgae cultivation in domestic sewage, aquaculture wastewater, industrial wastewater, etc., and successfully achieved the removal of nitrogen and phosphorus in water and the growth of microalgae. However, there are few reports on the cultivation of microalgae based on leachate for nutrient element recovery and biomass production.

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