Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of N, N-dimethylglycine DMG to treating or repairing peripheral nerve injury

A technology for peripheral nerve injury and dimethylglycine, which is applied in the field of biomedicine to achieve the effect of promoting growth and increasing DMG content

Pending Publication Date: 2021-12-21
NANTONG UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production of DMG in DRG tissues after peripheral nerve injury and its role in axon regeneration are rarely studied.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of N, N-dimethylglycine DMG to treating or repairing peripheral nerve injury
  • Application of N, N-dimethylglycine DMG to treating or repairing peripheral nerve injury
  • Application of N, N-dimethylglycine DMG to treating or repairing peripheral nerve injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Investigating the change of DMG content in DRG tissue after rat sciatic nerve injury

[0021] 1-1. Construction of adult SD rat sciatic nerve crush model

[0022] 192 healthy adult male SD rats, weighing 200 ± 20 g, were ordered from the Experimental Animal Center of Nantong University, and randomly divided into 4 groups, 48 ​​rats in each group. Rats were anesthetized by injecting compound anesthesia into the abdominal cavity (0.3 ml / 100 g), the left hind limb was shaved and disinfected with iodine, the sciatic nerve in the middle section of the left femur was exposed surgically, the nerve was separated from the muscle and basement membrane, and the special tweezers for pinching were clamped with full teeth The middle part of the sciatic nerve was held, the force of pinching was 54 N, and the time was 30 s. Then the wound was sutured, and 1 ml of normal saline and 0.2 ml of penicillin sodium solution were injected intraperitoneally. At the four time points o...

Embodiment 2

[0026] Example 2 DMG treatment in vitro promotes axonal growth of DRG neurons

[0027] 2-1. Extraction of primary DRG neurons

[0028] Several adult SD rats were anesthetized with a compound anesthetic, prepared hair and disinfected with 75% alcohol, dissected from the back tail, separated and taken out the complete spine, opened the upper lamina, took out all DRGs, and put them in with Hibernate A (HA+1% PS) in a dish; after all DRGs were taken out, discard HA, wash with PBS, discard PBS, add a small amount of collagenase, use ophthalmic scissors to fully cut DRG into pieces, and add collagenase to an appropriate amount (per 3 rats DRG plus 1 ml), placed at 37°C, 5% CO 2 In a constant temperature cell culture incubator for 90 min; transfer all the liquid in the dish to a 5 ml EP tube, centrifuge at 1200 rpm for 5 min, discard the supernatant, and add 0.25% Trypsin-EDTA preheated at 37°C in the same amount as collagenase (trypsin), pipette 10-20 times with a 1 ml pipette, an...

Embodiment 3

[0041] Example 3 DMG treatment in vivo promotes repair of sciatic nerve injury

[0042] Take 15 rats for sciatic nerve crush (the method is the same as in Example 1). After the crush (including the day of the crush), they were divided into three groups and given 0 / 10 / 100 mg / kg DMG (dissolved in normal saline) every day. After intraperitoneal injection, the rats were sacrificed on the fourth day (animal experiment procedures, such as Figure 4 Shown in A), the sciatic nerve on the injured side was taken out, embedded in OCT and frozen sectioned, the primary antibody anti-SCG10 (Novus, NBP1-49461, 1:400), the secondary antibody Alexa fluor 594 goat anti-rabbit IgG (H+L) ( ThermoFisher Scientific, A11012, 1:500), labeling DRG neurons to regenerate axons, and detecting the growth of axons after sciatic nerve injury.

[0043] Immunohistochemical detection of axon regeneration after intraperitoneal injection of different concentrations of DMG in rats after sciatic nerve injury, as ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to an application of N, N-dimethylglycine DMG to preparation of a medicine for treating or repairing peripheral nerve injury. The invention also discloses a method for verifying the effect of the N, N-dimethylglycine DMG in repairing peripheral nerve injury. The method comprises the following steps: detecting that the DMG in DRG tissue is obviously increased after ischial nerves of SD rats are pinched; using a DRG neuron in-vitro injury model to verify that the DMG can obviously promote the growth of pre-injured DRG neuron axons; and using an SD rat sciatic nerve pinch injury model to verify that the DMG can remarkably promote regeneration of DRG neuron axons after peripheral nerve injury. The invention provides a medicine for treating peripheral nerve injury, and provides a new possible treatment medicine for treating related nervous system diseases.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of N,N-dimethylglycine DMG in the preparation of medicines for treating or repairing peripheral nerve injuries. Background technique [0002] The activation of the intrinsic regenerative ability of neurons after peripheral nerve injury is affected by many factors, including metabolites in the cell body and surrounding microenvironment after neuron injury, and the key metabolites related to promoting axon regeneration of neurons in the peripheral nervous system will be Contributes to the repair and regeneration of peripheral nerves after injury. N, N-dimethylglycine N, N-dimethylglycine (DMG), as a nutritional additive and drug intermediate, participates in a variety of biological processes, and plays an important role in the body's cardiovascular function, immune function and improving exercise capacity. . However, the production of DMG in DRG tis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/198A61P25/02
CPCA61K31/198A61P25/02
Inventor 毛苏苏于彬张竣杰曹倩倩姜纯一
Owner NANTONG UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com