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Application of stachydrine and levo-stachydrine as characteristic markers of orchid flower honey

A technology of L-stachydrine and stachydine, which is applied in the directions of instruments, measuring devices, scientific instruments, etc., can solve the problems of unstable honey yield and adulteration, and achieves easy popularization and application, high specificity and sensitivity, and simple operation. Effect

Active Publication Date: 2022-04-01
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mituanhua blooms from February to April every year, and affected by climatic conditions, the production of Mituanhua honey is not stable. However, the high demand for Mituanhua honey in the market has led to the emergence of adulteration

Method used

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  • Application of stachydrine and levo-stachydrine as characteristic markers of orchid flower honey
  • Application of stachydrine and levo-stachydrine as characteristic markers of orchid flower honey
  • Application of stachydrine and levo-stachydrine as characteristic markers of orchid flower honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Development of Characteristic Markers of Rice Tuanhua Honey

[0039] In different years and from bee farms in different regions, a large number of rice ball honey samples and other varieties of honey samples were directly collected, and the UHPLC-Q-TOF MS method was used to detect the components of the honey samples. The method is as follows:

[0040] The honey sample was mixed with ultrapure water at a ratio of 1:5, and filtered with a membrane filter. A normal phase chromatographic column was used, mobile phase A was 0.1% formic acid aqueous solution, mobile phase B was methanol, and the flow rate was 0.3 mL / min. The mass spectrometry was electrospray mass spectrometry, the atomizing gas temperature was 300°C, the atomizing gas flow rate was 11 L / min, the atomizing gas pressure was 30 psi, the sheath gas temperature was 330°C, and the sheath gas flow rate was 12 L / min. The ionization voltage was 4000 kv, the fragmentation voltage was 138 V, and the scanning...

Embodiment 2

[0062] Example 2 Determination of the content of stachydrine and levo-stachydrine in different honey samples

[0063] Obtain 30 orchid honey and 75 other darker honey variety samples from the market, measure the stachydrine and levo-stachyline content of each honey sample according to the UHPLC-QQQ detection method in Example 1, The results are shown in Table 3, Table 4, Table 5 and Table 6.

[0064] Table 3 Determination of stachydrine and levo-stahydrine in honey samples (1)

[0065]

[0066] Note: "-" means not detected in honey samples.

[0067] Table 4 Determination of stachydrine and levo-stahydrine in honey samples (2)

[0068]

[0069] Note: "-" means not detected in honey samples.

[0070] Table 5 Determination of stachydrine and levo-stachyline content in honey samples (3)

[0071]

[0072] Note: "-" means not detected in honey samples.

[0073] Table 6 Determination of stachydrine and levo-stachyline content in honey samples (4)

[0074]

[0075] N...

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Abstract

The invention relates to the technical field of food detection, in particular to the application of stachydrine and levo-stachydrine as characteristic markers of orchid flower honey. In the present invention, stachydrine and levo-stachyline are firstly identified in rice ball honey, and these two alkaloid compounds can be used as characteristic marker compounds of rice ball honey to identify rice ball honey. The identification method has high specificity and sensitivity. The identification method of the orchid flower honey of the present invention has the characteristics of simple operation, high efficiency, etc., is convenient for popularization and application, and has great significance for the identification and authenticity evaluation of the orchid flower honey.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to the application of stachydrine and levo-stachydrine as characteristic markers of orchid flower honey. Background technique [0002] Plants of the genus Leucosceptrum in the family Lamiaceae are shrubs or small trees, mainly distributed in Yunnan, Sichuan, Bhutan, Nepal, India, Laos and Vietnam in China. Canum Smith (L. canum Smith) is a nectar plant whose roots and leaves have medicinal value. Rice ball flower honey is a natural sweet substance that is fully brewed after the Chinese bee collects rice ball flower nectar and mixes it with its own secretions. It is rich in nutrition and is very popular among consumers. The color of rice ball honey is very special, it is black, so rice ball honey is also called "black honey". Rice tuanhua blooms from February to April every year, and affected by climatic conditions, the production of rice ball honey is not stable. However, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 延莎王宣田文礼王凯吴黎明薛晓锋
Owner BEE RES INST CHINESE ACAD OF AGRI SCI