PCR fluorescent nucleic acid detection method and application thereof
A detection method and fluorescent nucleic acid technology, applied in the field of PCR fluorescent nucleic acid detection, can solve the problems of low integration and slow speed of nucleic acid detection, and achieve the effect of rapid screening
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Embodiment 1
[0079] The present invention provides PCR fluorescent nucleic acid detection method, please refer to figure 1 , shown as a flowchart of the method, including the following steps:
[0080] S1: The PCR nucleic acid amplification reaction of the sample is performed using a microfluidic chip;
[0081] S2: using blue light to excite fluorescently labeled molecules of the sample target, and using a PCR fluorescence detector to collect fluorescent signals from the microfluidic chip;
[0082] S3: Using yellow light to excite the fluorescently labeled molecules of the internal standard of the sample, and using the PCR fluorescence detector to collect fluorescent signals from the microfluidic chip;
[0083] S4: Perform image processing and image analysis based on the fluorescence signals collected by the PCR fluorescence detector, and perform result judgment.
[0084] As an example, the microfluidic chip used in the above step S1 is equipped with a thermal insulation tank structure, w...
Embodiment 2
[0103] This embodiment provides a PCR fluorescent nucleic acid detection device for implementing the PCR fluorescent nucleic acid detection method described in Embodiment 1.
[0104] see Figure 12 , which is a schematic structural diagram of the PCR fluorescent nucleic acid detection device, including a microfluidic chip 1, a blue LED assembly 2, a yellow LED assembly 3, a PCR fluorescence detector 4, a temperature control device 5 and a control circuit board 6, wherein the The microfluidic chip 1 is used to carry out the PCR nucleic acid amplification reaction of nucleic acid, the blue LED assembly 1 is located above the microfluidic chip 1, and is used to emit blue light to the surface of the microfluidic chip 1; the yellow LED assembly 3 Located above the microfluidic chip 1, it is used to emit yellow light to the surface of the microfluidic chip 1; the PCR fluorescence detector 4 is located above the microfluidic chip 1, and is located between the blue LED assembly 2 and ...
Embodiment 3
[0120] In this example, the PCR fluorescent nucleic acid detection method described in Example 1 is applied to gene detection. The detected objects include but are not limited to viruses, bacteria, cells, and nucleic acid substances in body fluids. Among them, the detected virus Including but not limited to the novel coronavirus COVID-19. Wherein, the PCR amplification process of the nucleic acid detection process is carried out in the PCR reaction chamber 1023 of the microfluidic chip.
[0121] In summary, the PCR fluorescent nucleic acid detection method of the present invention uses a microfluidic chip to perform the PCR nucleic acid amplification reaction of the sample, respectively uses blue light and yellow light to excite the fluorescent marker molecules of the sample target and internal standard, and uses a PCR fluorescence detector to collect samples from The fluorescent signal of the microfluidic chip is finally image-processed and analyzed based on the fluorescent s...
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Abstract
Description
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Application Information
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