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New application of notoginsenoside R1

A technology of notoginsenoside and acute lung injury, applied in the field of medicine development and preparation

Pending Publication Date: 2021-12-28
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies on the treatment of acute lung injury with notoginseng saponin R1 have not been reported

Method used

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  • New application of notoginsenoside R1
  • New application of notoginsenoside R1
  • New application of notoginsenoside R1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Grouping and administration of animal experiments

[0018] 50 C57BL / 6 male mice were randomly divided into 5 groups (10 in each group): blank control group, model group, notoginsenoside R1 treatment group including low-dose group (50mg / kg), middle-dose group (100mg / kg) kg), high dose group (200mg / kg). The mice in the model group and the treatment group were intraperitoneally injected with 10 mg / kg LPS once to construct a mouse model of acute lung injury; the mice in the blank group were injected intraperitoneally with the same volume of normal saline as in the model group; For drug treatment, the blank group and the model group were intragastrically administered distilled water every day, and the treatment group were intragastrically administered notoginseng saponin R1 50mg / kg, 100mg / kg, and 200mg / kg every day, for 21 consecutive days.

Embodiment 2

[0019] Example 2: Sample Collection

[0020] After 21 days, all the experimental mice were executed, and the weight of the mice was weighed before the execution. The lung tissues of the mice were taken out, washed with normal saline, and the excess water on the surface was wiped off, weighed, and the morphological changes of the lungs of the mice were observed. Normal mice had severe lung enlargement; after treatment with notoginseng saponin R1, the lung enlargement gradually improved, and the mouse lung index (mg / g)=lung weight was calculated according to the mouse body weight and lung weight when sacrificed (mg) / mouse body weight (g), the result is as follows figure 1 As shown, the lung index of the mice in the model group was much higher than that of the treatment groups.

Embodiment 3

[0021] Example 3: Pathological detection of mouse lung histopathology

[0022] The lung tissue was fixed with 10% neutral formaldehyde solution and dehydrated with ethanol gradient, transparent in xylene, embedded in paraffin, sliced ​​(thickness 4 μm), and the excised tissue slices were gently placed on 30% ethanol for the first exhibition. Then transfer the slides to a water bath at 45°C, stretch them for the second time, and attach them to the slides when the slices are completely unfolded; place the slices on a stand and dry them in a 37°C oven for subsequent H&E staining.

[0023] The specific operation steps of H&E staining are as follows: put the dried slices into xylene I and xylene II respectively for 5 minutes each, and dissolve the paraffin on the slices; take out the slices from xylene, and transfer them into absolute ethanol in sequence , 95% ethanol, 80% ethanol, and 70% ethanol, stay for 2 minutes each, and finally wash with running water for 5 minutes; move the...

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Abstract

The invention discloses new application of notoginsenoside R1, namely application of the notoginsenoside R1 in preparation of drugs for treating acute lung injury. Acute lung injury of mice is induced by intraperitoneal injection of lipopolysaccharide, an acute lung injury mouse model is established, and the influence of the notoginsenoside R1 on lung tissue pathological changes and related indexes after the acute lung injury of the mice is studied. Experimental results prove that the notoginsenoside R1 relieves and treats the acute lung injury of the mice by influencing expression of a lung tissue methyl CpG binding domain 2, and a preliminary basis is provided for application of the notoginsenoside R1 to treatment of the acute lung injury.

Description

technical field [0001] The invention belongs to the field of medicine development and preparation, and particularly relates to the application of notoginseng saponin R1 in the preparation of medicines for acute lung injury. Background technique [0002] Acute lung injury (ALI) is a variety of direct and indirect injury factors, resulting in pulmonary capillary endothelial cells and alveolar epithelial cell damage resulting in diffuse pulmonary interstitial and alveolar edema, acute hypoxic respiratory insufficiency or Respiratory failure, acute respiratory distress syndrome (Acute respiratory distress syndrome, ARDS) can occur after damage to a certain extent. The essence of the pathogenesis of ALI / ARDS is an uncontrolled inflammatory response, which leads to diffuse lung parenchymal damage and respiratory dysfunction as the main pathological features. Lung injury will increase the inflow of reactive oxygen species and lead to the release of inflammatory mediators TNF-α, IL...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61K36/258A61P11/00
CPCA61K31/704A61K36/258A61P11/00
Inventor 葛锋王娟李捷方昱文曲媛崔秀明
Owner KUNMING UNIV OF SCI & TECH
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