Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of polyethyleneimine compound in luminescence method in-vitro diagnostic reagent

A technology of polyethyleneimine and in vitro diagnosis, which is applied in chemiluminescence/bioluminescence, analysis and measurement devices through chemical reactions of materials, and can solve the sensitivity and low precision interference of kits and uneven distribution of particles Polymerization, high background and other problems, to achieve the effect of expanding the coating surface area, speeding up the reaction speed, and improving the sensitivity

Pending Publication Date: 2021-12-31
NINGBO RUI BIO TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing tubular chemiluminescence uses magnetic or non-magnetic particles as the coating carrier, but there are problems such as uneven particle distribution and aggregation
The use of acridinium esters in direct chemiluminescence can simplify the detection process and reduce costs, but it will cause low sensitivity in detection
In fact, in the development process of the existing chemiluminescent reagents, due to the high sensitivity requirements of some specific projects, the large background variation will cause great interference to the kit sensitivity and low value precision, resulting in background bias. High and False Positive Issues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 15ml of magnetic particles with a particle size of 2 μm, wash and resuspend, add 60ul of EDC and NHS each, mix well and incubate for 20min to obtain activated magnetic particles. Add 15 mg of cTnI monoclonal antibody I to be cross-linked to the activated magnetic particles, and incubate for 3 h with MES buffer (100 mM) as the reaction medium. The supernatant was removed by magnetic adsorption, and the product was resuspended by adding a Tris solution containing 0.07% PEG-g-PEI and 0.9% NaCl, and blocked by shaking for 7 hours. Afterwards, magnetic separation and ultrasound were used to wash the magnetic microspheres coupled with the antibody, and diluent was used to dilute to a concentration of 10 mg / ml to preserve R1. The diluent is PBS 20mM, 0.5% BSA, 0.2% TW-20, 0.9% NaCl, 5% trehalose, 0.1% NaN 3 , pH=7.1. The sensitivity of the diagnostic reagent prepared by this embodiment reaches 0.9 pg / ml when used.

Embodiment 2

[0036] Compared with Example 1, the difference is that PEG-g-PEI is 0.01% PEG-g-PEI. The sensitivity of the diagnostic reagent prepared by this embodiment reaches 1.4 pg / ml when used.

Embodiment 3

[0038] Compared with Example 1, the difference is that PEG-g-PEI is 0.2% PEG-g-PEI. The sensitivity of the diagnostic reagent prepared by this embodiment reaches 1.8 pg / ml when used.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to application of a polyethyleneimine compound in a luminescence method in-vitro diagnostic reagent, and belongs to the technical field of medical reagents. According to the invention, the in-vitro diagnostic reagent comprises R1 and / or R2, the R1 is prepared by using the polyethyleneimine compound as a sealing agent, and the R2 is prepared by using the polyethyleneimine compound as an intermediate carrier. The in-vitro diagnostic reagent prepared by the invention can realize low background noise and high marking efficiency, and achieves the purpose of improving the sensitivity of a diagnostic result.

Description

technical field [0001] The invention belongs to the technical field of medical reagents, and relates to the application of polyethyleneimine compounds in luminescent in vitro diagnostic reagents. Background technique [0002] Chemiluminescence is a kind of light radiation phenomenon accompanied by the chemical reaction of substances. The substances measured by chemiluminescence analysis can be divided into three categories: the first category is the reactant in the chemiluminescence reaction; the second category is the catalyst, sensitizer or inhibitor in the chemiluminescence reaction; the third category is the coupling Reactants, catalysts, sensitizers, etc. in the reaction. These three types of substances can also be used to measure other substances through labeling, further expanding the application range of chemiluminescence analysis. [0003] As a new chemiluminescence analysis method, magnetic particle chemiluminescence immunoassay technology combines magnetic separ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/543
CPCG01N21/76G01N33/54326
Inventor 周广亮张闻陈媛陈烨徐孝锋陈远昊
Owner NINGBO RUI BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com