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Cell nucleolus imaging red fluorescent carbon dot and preparation method and application thereof

A red fluorescence and cell nucleus technology, applied in the field of nanomaterials, can solve the problems of lack of probes and affect the research progress of nucleolus-related life activities, and achieve the effects of large Stokes shift, good photostability, and simple preparation methods

Pending Publication Date: 2022-01-04
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As far as we know, there is an extreme lack of probes that can achieve high-quality fluorescence imaging of nucleoli in animals, which seriously affects the research progress of nucleolus-related life activities in vivo

Method used

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  • Cell nucleolus imaging red fluorescent carbon dot and preparation method and application thereof
  • Cell nucleolus imaging red fluorescent carbon dot and preparation method and application thereof
  • Cell nucleolus imaging red fluorescent carbon dot and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] The preparation of red fluorescent carbon dots comprises the following steps:

[0051] (1) Preparation of raw materials: weigh Congo red and p-phenylenediamine so that the mass ratio is 1:2, dissolve them in water respectively (just dissolve) and mix well and transfer to a hydrothermal reaction kettle ;

[0052] (2) Reaction: React in a hydrothermal reactor at 200°C for 8 hours to form a carbon dot solution;

[0053] (3) Purification: Centrifuge the carbon dot solution at a speed of 14000rpm for 10min, take the supernatant and dialyze it with a dialysis bag with a molecular weight cut-off of 1000Da at room temperature for 4h to obtain a water dispersion of red fluorescent carbon dots, which is the target fluorescent carbon dot dispersion . Afterwards, take 5mL of the carbon dot dispersion and freeze-dry it with a freeze dryer to obtain a solid carbon dot powder. Use a microbalance to weigh the mass of the carbon dot powder to calculate the concentration of the prepare...

Embodiment 2

[0056] The preparation steps of the fluorescent carbon dots in Example 2 are the same as those in Example 1, except that the ratio of Congo red to semi-p-phenylenediamine in step (1) is 1:1.

Embodiment 3

[0058] The preparation steps of the fluorescent carbon dots in Example 3 are the same as in Example 1, except that the molar ratio of Congo red to semi-p-phenylenediamine in step (1) is 1:3.

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Abstract

The invention discloses a cell nucleolus imaging red fluorescent carbon dot and a preparation method and application thereof. The red fluorescent carbon dot is prepared from the following raw materials in parts by weight: 1 part of Congo red and 1-10 parts of p-phenylenediamine. The fluorescent carbon dot is prepared by taking p-phenylenediamine and Congo red as raw materials and adopting a one-step hydrothermal method, and the carbon dot prepared by the invention can realize rapid, no-clean, long-time and light-stable (anti-bleaching) imaging of cell nucleuses and nucleuses of mammalian cells, and can also be applied to living cell nucleolus fluorescence imaging of model animals (such as zebra fish and nematodes). In addition, the carbon dot also has the advantages of low preparation cost, good water dispersibility, low cytotoxicity, large Stokes shift and the like, and can replace the commercialized nucleolus fluorescent probe which is widely used at present.

Description

technical field [0001] The invention belongs to nanomaterials, in particular to a red fluorescent carbon dot for cell nucleolus imaging and its preparation method and application. Background technique [0002] Ribonucleic acid (RNA) is widely distributed in the cytoplasm and nucleus, and the RNA in the nucleus is mainly enriched in the nucleolus. RNA plays an important role in the life activities of cells. For example, as messenger RNA (mRNA), it is responsible for transmitting genetic information and directly directing protein synthesis during protein synthesis; as transfer RNA (tRNA), it is responsible for transporting amino acids and interpreting proteins during protein synthesis. mRNA genetic code; as ribosomal RNA (rRNA) and ribosomal protein to form ribosomes; as RNase to regulate gene expression and catalyze biochemical reaction processes, etc. The nucleolus structure is ubiquitous in the eukaryotic nucleus, and its main components include rDNA, rRNA and ribonucleopr...

Claims

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Application Information

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IPC IPC(8): C09K11/65C01B32/15A61K49/00A61K47/52G01N21/64
CPCC09K11/65C01B32/15G01N21/6428G01N21/6458A61K49/0019A61K49/006A61K47/52C01P2002/01C01P2004/04
Inventor 吴富根许可飞贾浩然
Owner SOUTHEAST UNIV
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