Enzymolysis accelerant for improving enzymolysis efficiency of cellulose and application of enzymolysis accelerant

A cellulase and accelerator technology, which is applied in the field of efficient conversion and utilization of biomass, can solve the problems of poor synergistic effect, high use cost, and reduced cellulase activity, so as to shorten the enzymatic hydrolysis time, improve the efficiency, The effect of reducing the amount of enzymes

Pending Publication Date: 2022-01-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the later stage of enzymatic hydrolysis, the activity of cellulase is significantly reduced, and the effect still needs to be further improved.
Other studies have shown that there are some proteins that can reduce the amount of cellulase, but there are also problems of high cost and poor synergistic effect

Method used

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  • Enzymolysis accelerant for improving enzymolysis efficiency of cellulose and application of enzymolysis accelerant

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0036] Take 2 grams (on a dry basis) of the pretreatment residue, add 10FPU / g of cellulase (Saili II), 30mg / g of Tween 80 additive and 100mL of acetic acid-sodium acetate buffer solution with pH=4.8 enzymolysis. During the enzymolysis process, the temperature was controlled at 50° C., and the rotation speed was 150 rpm. After 24 hours, 48 ​​hours and 72 hours of enzymatic hydrolysis, 1mL samples were taken out and inactivated. The glucose concentration in the enzymolysis solution was measured by high performance liquid chromatography, and the calculated glucose yields were 84.06%, 92.17% and 92.32%, respectively.

example 2

[0038] With the method of Example 1, the difference is that the additive added in the enzymolysis process is 40mg / g tea saponin, after enzymolysis is carried out 24 hours, 48 ​​hours and 72 hours, 1mL samples are taken out and inactivated The concentration of glucose in the enzymolysis solution was measured by phase, and the calculated glucose yields were 87.88%, 92.18% and 93.53%, respectively.

example 3

[0040] With the method of example 1, the difference is that the additive added in the enzymolysis process is 40mg / g of BSA, after enzymolysis is carried out 24 hours, 48 ​​hours and 72 hours, take out 1mL sample and carry out inactivation treatment, measure with high performance liquid phase The glucose concentration in the enzymatic hydrolysis solution and the calculated glucose yields were 88.15%, 92.16% and 93.09%, respectively.

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Abstract

The invention discloses an enzymolysis accelerant for improving the enzymolysis efficiency of cellulose and application of the enzymolysis accelerant. The enzymolysis accelerant is prepared from Tween, tea saponin, protein and xylanase. Data of the accelerant show that different from the accelerant for remarkably improving the enzymolysis efficiency of the cellulase by independently adding the Tween, the tea saponin and the protein, the enzymolysis efficiency of the cellulase cannot be improved by purely adding the xylanase, but the enzymolysis efficiency of the cellulase is slightly reduced. After the xylanase is compounded with the Tween, the tea saponin and the protein, the later-stage enzymolysis efficiency of the cellulase is improved unexpectedly, the continuous action time of the cellulase is prolonged, and an unexpected effect is achieved; and according to some examples of the invention, the glucose yields after 24-hour enzymolysis and 48-hour enzymolysis can respectively reach 89.55% and 96.46%, and the cellulose enzymolysis time can be effectively shortened from 72 hours to 24 hours. According to some examples of the invention, the action time of cellulase is prolonged while the enzymolysis time is shortened.

Description

technical field [0001] The invention relates to the technical field of high-efficiency conversion and utilization of biomass, in particular to an enzymolysis accelerator for improving cellulose enzymolysis efficiency and a method for enzymatic cellulose hydrolysis using the enzymolysis accelerator. Background technique [0002] With the reduction of fossil energy, the pollution of the environment and the enhancement of environmental awareness, people are forced to look for a new clean energy. As one of them, biomass energy has attracted people's attention. Cellulose is a common biomass that can be enzymatically hydrolyzed into glucose by cellulase. It has a wide range of uses and has attracted more and more attention. [0003] The amount of cellulase and the efficiency of enzymatic hydrolysis have a crucial impact on the cost of cellulose enzymatic hydrolysis. The less the amount of cellulase used, the faster the rate of enzymatic hydrolysis (the shorter the time), which i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P19/14
CPCC12P19/02C12P19/14C13K1/02C12P2201/00
Inventor 张红丹张贤滨谢君李晓垠
Owner SOUTH CHINA AGRI UNIV
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