Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for detecting content of microcrystalline cellulose in collagen casing

A technology of microcrystalline cellulose and collagen, which is applied in the field of collagen detection, can solve the problems of heavy detection data workload, unsatisfactory detection results, high detection error rate, etc., to save experimental costs, shorten digestion time, and high detection efficiency Effect

Pending Publication Date: 2022-01-07
山东海奥斯生物科技股份有限公司
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of microcrystalline cellulose is an important physical index in casings, but the current measurement methods have the following problems: the workload of detection data is large, each detection is time-consuming and labor-intensive, the detection cost is high, the detection error rate is high, and the detection results Not ideal, seriously affecting the quality of the product

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting content of microcrystalline cellulose in collagen casing
  • Method for detecting content of microcrystalline cellulose in collagen casing
  • Method for detecting content of microcrystalline cellulose in collagen casing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for detecting microcrystalline cellulose content in collagen casings, comprising the following steps:

[0031] (1) Cut the collagen sausage casing into the Erlenmeyer flask, record the casing weight M1; add 10wt% hydrochloric acid and collagen proteolytic enzyme, put a funnel on the Erlenmeyer flask, and heat in a water bath at 75°C for 1 hour;

[0032] Then dip a small amount of solution and observe under the microscope whether the digestion is complete, and the cellulose is completely exposed as the digestion is complete;

[0033] The volume-to-mass ratio of the hydrochloric acid to the collagen casing is 5mL:2g.

[0034] The dosage of collagen proteolytic enzyme is 0.16%, and the dosage of 10% hydrochloric acid is 5mL.

[0035] (2) Pour the digested solution into a centrifuge tube, and centrifuge at 3500rpm for 25 minutes; too much solution cannot be loaded at one time, centrifuge in several times, pour out the supernatant after each centrifugation, and the...

Embodiment 2

[0039] A method for detecting microcrystalline cellulose content in collagen casings, comprising the following steps:

[0040] (1) Cut the collagen casing into the Erlenmeyer flask, record the casing weight M1; add 10wt% hydrochloric acid and collagen proteolytic enzyme, put a funnel on the Erlenmeyer flask, and heat in a water bath at 80°C for 3 hours;

[0041] Then dip a small amount of solution and observe under the microscope whether the digestion is complete, and the cellulose is completely exposed as the digestion is complete;

[0042] The volume-to-mass ratio of the hydrochloric acid to the collagen casing is 5mL:2g.

[0043] The dosage of collagen proteolytic enzyme is 0.16%, and the dosage of 10% hydrochloric acid is 5mL.

[0044] (2) Pour the fully digested digestion solution into a centrifuge tube and centrifuge at 4000rpm for 30 minutes; too much solution cannot be loaded at one time, centrifuge in several times, pour out the supernatant after each centrifugation,...

Embodiment 3

[0048] A method for detecting microcrystalline cellulose content in collagen casings, comprising the following steps:

[0049] (1) Cut the collagen sausage casing into the Erlenmeyer flask, record the casing weight M1; add 10wt% hydrochloric acid and collagen proteolytic enzyme, put a funnel on the Erlenmeyer flask, and heat in a water bath at 85°C for 4 hours;

[0050] Then dip a small amount of solution and observe under the microscope whether the digestion is complete, and the cellulose is completely exposed as the digestion is complete;

[0051] The volume-to-mass ratio of the hydrochloric acid to the collagen casing is 5mL:2g.

[0052] The dosage of collagen proteolytic enzyme is 0.16%, and the dosage of 10% hydrochloric acid is 5mL.

[0053] (2) Pour the fully digested digestion solution into a centrifuge tube and centrifuge at 4500rpm for 35 minutes; too much solution cannot be loaded at one time, centrifuge in several times, pour out the supernatant after each centrif...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of collagen detection and particularly relates to a method for detecting the content of microcrystalline cellulose in collagen casings. The method comprises the following steps of (1) cutting a collagen casing into a conical flask, and recording the weight M1 of the casing; adding 10wt% hydrochloric acid and a protease preparation, putting a funnel on a conical flask, and heating in a water bath; (2) pouring the completely digested digestion solution into a centrifuge tube for centrifugation; pouring out supernate after each time of centrifugation, then pouring the residual digestion solution for centrifugation, and adding distilled water for centrifugal washing; (3) weighing the dried weighing bottle, recording as M2, pouring the centrifuged substrate into the dried weighing bottle, washing with distilled water, drying the weighing bottle filled with the substrate in a drying oven, and recording as M3; and (4) calculating the content of microcrystalline cellulose. The device is scientific and reasonable in design, simple and easy to operate, high in detection efficiency and detection accuracy, time-saving and labor-saving, detection cost is reduced, and the product quality is improved.

Description

technical field [0001] The invention relates to the technical field of collagen detection, in particular to a method for detecting the content of microcrystalline cellulose in collagen casings. Background technique [0002] The main component of microcrystalline cellulose is linear polysaccharides bound by β-1,4-glucosidic bonds. In general plant fibers, microcrystalline cellulose accounts for about 70%, and the other 30% is amorphous. [0003] In casings, it is also rich in microcrystalline cellulose. The content of microcrystalline cellulose is an important physical index in casings, but the current measurement methods have the following problems: the workload of detection data is large, each detection is time-consuming and labor-intensive, the detection cost is high, the detection error rate is high, and the detection results Not ideal, seriously affecting the quality of the product. Contents of the invention [0004] The technical problem to be solved by the present...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/00G01N21/84G01N1/40
CPCG01N5/00G01N21/84G01N1/4044
Inventor 赵换英崔浩冉李智超宋立国马龙
Owner 山东海奥斯生物科技股份有限公司