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A kind of efficient and safe preparation method and application of brain protein hydrolyzate

A cerebroprotein hydrolysate, pig brain technology, applied in the field of biomedicine, can solve the problems affecting the quality of drugs, the stability of drug safety, the long cycle of enzymatic hydrolysis and filtration, the increase of high molecular weight substances, etc. Risk of infection, shortened production cycle, good effect of extract quality

Active Publication Date: 2022-06-24
BEIJING SIHUAN PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pig brain extract has high requirements on temperature control, filtration effect, preparation time and container handling during the preparation process, which may easily lead to too long enzymatic hydrolysis and filtration cycle and low extraction efficiency, which may easily lead to microbial contamination. and high-molecular-weight substances increase, thereby affecting the quality, stability and drug safety of drugs

Method used

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  • A kind of efficient and safe preparation method and application of brain protein hydrolyzate
  • A kind of efficient and safe preparation method and application of brain protein hydrolyzate
  • A kind of efficient and safe preparation method and application of brain protein hydrolyzate

Examples

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Effect test

Embodiment 1

[0039] Example 1 Preparation of Troxerutin Cerebroprotein Hydrolysate Injection

[0040] The preparation method of efficient and safe Troxerutin cerebroprotein hydrolysate injection comprises the following steps:

[0041] (1) Select healthy pig brains that have passed the quarantine and are frozen below -20°C, thaw them at below 10°C, and remove impurities. According to the mass ratio of pig brain:water of 1:1, add water, The homogenate was minced, and the obtained porcine brain homogenate was frozen at -20°C;

[0042] (2) placing the frozen homogenate obtained in step (1) in a water bath at 15°C for 48 hours, then heating at 95°C for 30 minutes, cooling to 40°C, adjusting the pH of the pig brain homogenate to 7.0, adding 0.5% After compounding the enzyme, it was placed in a water bath at 40°C for 5 hours, then cooled to 25°C, adjusted to pH 3.5, and then cooled to 5°C to obtain an enzymatic hydrolysis solution, wherein the compound enzyme was pancreatic Protease: lipase: a...

Embodiment 2

[0047] Example 2 Preparation of Troxerutin Cerebroprotein Hydrolysate Injection

[0048] The preparation method of efficient and safe Troxerutin cerebroprotein hydrolysate injection comprises the following steps:

[0049] (1) Select healthy pig brains that have passed the quarantine, thaw them at below 10°C, and remove impurities. According to the mass ratio of pig brain:water of 1:2, add water, homogenize and mince them, and then prepare The porcine brain homogenate was frozen at -20°C;

[0050] (2) The frozen homogenate obtained in step (1) was placed in a 15°C water bath for 48 hours, then heated at 95°C for 30 minutes, cooled to 30°C, adjusted to pH 6.5 of the porcine brain homogenate, and added with 0.1% (mass ratio) of the composite enzyme, and then placed in a 30°C water bath for 1-10 hours for enzymatic hydrolysis, then cooled to 20°C, adjusted to pH 3.0, and then cooled to 5°C to obtain an enzymatic hydrolysis solution, wherein , the composite enzyme is trypsin: l...

Embodiment 3

[0055] Example 3 Preparation of Troxerutin Cerebroprotein Hydrolysate Injection

[0056] The preparation method of efficient and safe Troxerutin cerebroprotein hydrolysate injection comprises the following steps:

[0057] (1) Select healthy pig brains that have passed the quarantine, thaw them at below 10°C, and remove impurities. According to the mass ratio of pig brain:water of 1:3, add water, homogenize and mince them, and then prepare The porcine brain homogenate was frozen at -20°C;

[0058] (2) The frozen homogenate obtained in step (1) was placed in a 15°C water bath for 48 hours, then heated at 95°C for 30 minutes, cooled to 50°C, adjusted to pH 8.0 of the porcine brain homogenate, and added with 1.0% (mass ratio) of the composite enzyme, put it in a water bath at 50 °C for 10 hours, and then cool down to 20-30 °C, adjust the pH to 4.0, and then cool down to 6 °C to obtain an enzymatic hydrolysis solution, wherein , the composite enzyme is trypsin: lipase: amylase in...

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Abstract

The present invention relates to an efficient and safe preparation method of pig brain extract, comprising the following steps: (1) selecting healthy pig brain, thawing it at a temperature below 10°C, and following the pig brain:water mass ratio of 1:1 ‑3, add water, grind the homogenate, and freeze at ‑20°C; (2) put the frozen homogenate in a water bath at 80‑95°C to keep warm and melt, add 0.1‑1.0% compound enzyme, and then freeze it Put it in a water bath at 30-50°C and heat it for 1-10 hours to obtain the enzymolysis solution; (3) Take out the enzymolysis solution and centrifuge at 0-10°C and 4000-4500r / min for 20-30min, collect and centrifuge Supernatant, then put the prepared centrifuged supernatant in a pulp filter for vacuum filtration, and freeze the collected filtrate at -20°C; (4) Take out the frozen filtrate, put it in a water bath below 15°C to melt , then place the melted filtrate at 0-10°C and centrifuge at 4000-4500r / min for 20-30min, collect the filtrate and filter it with a pulp filter under reduced pressure, and the obtained filtrate is passed through an ultra- The pig brain extract was obtained by membrane ultrafiltration. The invention significantly improves the resource utilization rate, significantly improves the stability, quality uniformity, effectiveness and safety of the troxerutin cerebroprotein hydrolyzate injection, and ensures safe and effective clinical medication.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an efficient and safe preparation method of cerebroprotein hydrolysate and its application. Background technique [0002] Troxerutin Cerebroprotein Hydrolysate Injection is a sterile injection prepared by combining Troxerutin and porcine brain extract. Each 1000ml Troxerutin Cerebroprotein Hydrolysate Injection is composed of an appropriate amount of pig brain extract (containing total nitrogen 500mg, polypeptide 1910mg, nucleic acid ≥800mg), 40g Troxerutin (with C 33 H 42 O 19 meter) and an appropriate amount of water for injection. Troxerutin (C 33 H 42 O 19 ) is 36.0-44.0mg / ml, the total nitrogen content is 0.40-0.60mg / ml, the polypeptide content is 1.52-2.30mg / ml, and the nucleic acid content is ≥0.8mg / ml. Troxerutin Cerebroprotein Hydrolysate Injection is clinically used for the treatment of acute and chronic cerebrovascular diseases such as cerebral thrombosis,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/30A61K38/01A61K9/08A61P9/10A61P9/14A61P25/00
CPCA61K35/30A61K38/012A61K9/08A61K9/0019A61P9/10A61P25/00A61P9/14
Inventor 王建辉杨阳赵友明王洪敏宋春蕾
Owner BEIJING SIHUAN PHARMA
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