Amniotic fluid proteomics detection method of premature delivery prediction marker and application thereof

A technology of proteomics and detection methods, applied in the field of amniotic fluid proteomics detection of preterm birth prediction markers, can solve the problem of unclear regulatory mechanism and other problems

Pending Publication Date: 2022-01-14
MATERNAL & CHILD HEALTH CARE HOSPITAL OF SHANDONG PROVINCE SHANDONG UNIV
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Problems solved by technology

[0008] (3) In the prior art, the regulation mechanism of serine protease inhibitor A1 (SEPRIN-A1) and insulin-like growth factor binding protein 4 (IGFBP-4) is unclear

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  • Amniotic fluid proteomics detection method of premature delivery prediction marker and application thereof
  • Amniotic fluid proteomics detection method of premature delivery prediction marker and application thereof
  • Amniotic fluid proteomics detection method of premature delivery prediction marker and application thereof

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Embodiment

[0151] Analysis of amniotic fluid can predict a variety of pregnancy complications based on information revealing underlying biological mechanisms.

[0152] The invention adopts liquid chromatography-mass spectrometry combined analysis technology to detect the differentially expressed proteins in the amniotic fluid of premature patients (excluding cervical factors) and full-term normal control pregnant women. 44 proteins related to preterm birth and 9 signaling pathways that may be involved were identified by GO and KEGG analysis, and 8 interested proteins were selected for further analysis. Enzyme-linked immunosorbent assay was used to verify the expression of selected candidate proteins; ELISA analysis was performed in another cohort to verify the expression of these proteins. The present invention found that IGFBP-4, renin, SEPRINA1 and TIPM-1 are potential biomarkers of SPTD associated with spontaneous PTD after exclusion of cervical insufficiency <34 weeks.

[0153] Amon...

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Abstract

The invention discloses an amniotic fluid proteomics detection method of a premature delivery prediction marker and application thereof and relates to the technical field of genetic engineering. The method comprises the following steps of obtaining amniotic fluid differential expression proteins of premature delivery patients (excluding cervical factors) and full-term delivery normal control pregnant women through a liquid chromatography-mass spectrometry method; carrying out GO functional characterization and KEGG pathway analysis on the differentially expressed protein; and verifying the selected candidate protein by using an enzyme-linked immunosorbent assay. The TMT-labeled quantitative proteomics method is used for comparing protein expression differences in amniotic fluid of premature delivery patients (excluding cervical factors) and amniotic fluid of full-term delivery pregnant women. Bioinformatics analysis shows that 44 differential expression proteins and 9 main ways may participate in premature delivery. Subsequent ELISA detection proves that SEPRIN-A1 and IGFBP-4 have significant correlation with premature delivery, and a new thought and approach are provided for prevention and mechanism clarification of premature delivery.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an amniotic fluid proteomics detection method and application of premature birth prediction markers. Background technique [0002] Preterm birth is defined as delivery after 28 weeks of gestation but before 37 weeks. Worldwide, more than 15 million premature babies are born each year, and more than 1 million of them die prematurely. The mechanism of premature birth is still unclear. Bacterial infection, decidua hemorrhage and vascular disease, decidua aging, abnormal maternal-fetal immune tolerance, and progesterone withdrawal can all lead to early labor initiation, but the underlying etiology has not been clearly elucidated. [0003] Studies have shown that 75%-85% of premature births are spontaneous without a clear cause, and most premature births diagnosed early can be prevented. Therefore, there is an urgent need to find biomarkers for the preventio...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86G01N33/68C12P21/06
CPCG01N30/02G01N30/06G01N30/34G01N30/72G01N30/8675G01N33/6848C12P21/06G01N2030/067
Inventor 王谢桐李安娜张美华房振亚
Owner MATERNAL & CHILD HEALTH CARE HOSPITAL OF SHANDONG PROVINCE SHANDONG UNIV
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