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Preparation method of C protein detection test paper and handheld reading device

A protein detection and reading device technology, applied in the field of preparation of protein C detection test paper, can solve the problems of light flickering, affecting the accuracy of detection results, unstable detection signals, etc., and achieve the effect of ensuring accuracy

Pending Publication Date: 2022-01-14
XIAN TECHNOLOGICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, conventional fluorescent particles have problems such as photobleaching and light flickering, and the detection signal is unstable.
Moreover, most of these fluorescent particles are excited by ultraviolet or visible light. Under such excitation light conditions, whole blood samples will produce serious background fluorescence interference, which will affect the accuracy of the detection results.

Method used

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  • Preparation method of C protein detection test paper and handheld reading device
  • Preparation method of C protein detection test paper and handheld reading device
  • Preparation method of C protein detection test paper and handheld reading device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Taking the NC membrane with an average pore size of 15.52 μm for CRP protein detection as an example, the effect of the method of the present invention is illustrated, and the specific experimental process includes the following steps:

[0057] 1) Preparation of rare earth-doped UCNPs by thermal decomposition method;

[0058] 2) Using a polymer encapsulation method, using phospholipid polyethylene glycol carboxyl (DSPE-PEG-COOH) to modify the surface of the hydrophobic UCNPs prepared in step 1);

[0059] 3) using a condensation reaction to combine the surface-modified water-soluble UCNPs in step 2) with CRP antibodies to prepare UCNPs-CRP antibody probes;

[0060] 4) Using an XYZ three-dimensional gold-spraying film-drawing apparatus, evenly spray the probe of the UCNPs-CRP antibody prepared in step 3) on the binding pad, and dry it for later use;

[0061] 5) Dilute the CRP Ab7# antibody and goat anti-mouse IgG with PBS buffer solution as the test line (Test line, T li...

Embodiment 2

[0070] Taking an NC membrane with an average pore size of 8.50 μm for CRP protein detection as an example, the effect of the method of the present invention is illustrated, and the specific experimental process includes the following steps:

[0071] With reference to steps 1), 2), 3), 4), and 5) in Example 1;

[0072] 6) Paste the NC membrane (average pore size: 8.50μm, average capillary flow time: 94.98s, porosity: 53.46%) on the PVC bottom plate, and use the gold spraying device to spray the T and C line solutions prepared in step 5) Fixed on the NC film surface of the lateral flow test paper in the shape of a rectangular strip to form a detection line, dried for later use;

[0073] 7) Install the bonding pad prepared in step 4), the NC film prepared in step 6), the sample pad, and the water-absorbing pad on the PVC base plate, cut out lateral flow test strips with a width of 3.9 mm, and put them in the case for later use;

[0074] 8) Prepare a CRP antigen solution detectio...

Embodiment 3

[0080] Taking an NC membrane with an average pore size of 6.49 μm for CRP protein detection as an example, the effect of the method of the present invention is illustrated, and the specific experimental process includes the following steps:

[0081] With reference to steps 1), 2), 3), 4), and 5) in Example 1;

[0082] 6) Paste the NC membrane (average pore size: 6.49μm, average capillary flow time: 138.97s, porosity: 51.87%) on the PVC bottom plate, and use the gold spraying device to spray the T and C line solutions prepared in step 5) Fixed on the NC film surface of the lateral flow test paper in the shape of a rectangular strip to form a detection line, dried for later use;

[0083] 7) Install the bonding pad prepared in step 4), the NC film prepared in step 6), the sample pad, and the water-absorbing pad on the PVC base plate, cut out lateral flow test strips with a width of 3.9 mm, and put them in the case for later use;

[0084] 8) Prepare a CRP antigen solution detecti...

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Abstract

The invention discloses a preparation method of C protein detection test paper, namely a handheld reading device. The preparation method comprises the following steps: pasting an NC membrane on a substrate of lateral flow test paper; fixing a prepared detection solution in a detection area on the NC membrane, and fixing the prepared contrast solution in a contrast area on the NC membrane; attaching a combination pad and a water absorption pad to the positions, located on the two sides of the NC membrane, of the substrate respectively, wherein the combination pad and the water absorption pad are both connected with the NC membrane, and a probe of a UCNPs-CRP antibody is arranged on the combination pad; and attaching a sample pad to the position, located on the other side of the combination pad, of the substrate, wherein the sample pad is connected with the combination pad. According to the invention, the UCNPs-CRP antibody probe is arranged on the combination pad, so that the influence of background fluorescence generated by a whole blood sample on the detection performance can be avoided; and the detection area and the control area are arranged on the NC membrane, so that the accuracy of a detection result can be ensured.

Description

technical field [0001] The invention belongs to the technical field of preparation of protein C detection test paper, and in particular relates to a preparation method of protein C detection test paper and a handheld reading device. Background technique [0002] C-reactive protein (C-reactive protein, CRP) is present in the blood, can reflect the infection of bacteria and viruses, and an important indicator reflecting the degree of disease development. In recent years, it has been proved that it is related to the development of various diseases. Therefore, if CRP detection can be performed quickly, sensitively, accurately, portable and at low cost, the efficiency of disease diagnosis can be effectively improved and the economic pressure of patients can be reduced. [0003] In recent years, real-time detection technology has been widely concerned by researchers because of its portable detection equipment, real-time information feedback and low equipment cost. In particular, ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/558G01N33/533G01N33/52
CPCG01N33/68G01N33/558G01N33/533G01N33/52G01N2333/4737
Inventor 金碧瑞张楚瑶徐嘉珧罗楚瑜
Owner XIAN TECHNOLOGICAL UNIV
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