Application of Houttuynia cordata Thunb. polysaccharide in preparation of medicine for preventing and treating inflammatory bowel disease

A technology of inflammatory bowel disease and Houttuynia cordata, which is applied in the field of Houttuynia cordata polysaccharides in the preparation of drugs for the prevention and treatment of inflammatory bowel disease, and can solve the problems of the efficacy of Houttuynia cordata inflammatory bowel disease and drug research reports that have not been seen.

Pending Publication Date: 2022-01-18
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Looking at the research at home and abroad, the research on Houttuynia cordata is mostly seen in the research on lung diseases, and there is no report on the efficacy of Houttuynia cordata polysaccharides in treating inflammatory bowel disease and its drug research

Method used

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  • Application of Houttuynia cordata Thunb. polysaccharide in preparation of medicine for preventing and treating inflammatory bowel disease
  • Application of Houttuynia cordata Thunb. polysaccharide in preparation of medicine for preventing and treating inflammatory bowel disease
  • Application of Houttuynia cordata Thunb. polysaccharide in preparation of medicine for preventing and treating inflammatory bowel disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: Preparation of Houttuynia cordata polysaccharide

[0053] Take Houttuynia cordata Thunb. 100g medicinal material, pulverize, extract by cold soaking with 95% ethanol for 3 times, put the dregs in a ventilated place at room temperature to dry, then extract with hot water for 3 times, filter, combine the extracts, Concentrate, centrifuge, remove free protein from the supernatant with trichloroacetic acid, centrifuge, dialyze the supernatant with water for 3 days, concentrate the dialysate to a small volume, add ethanol until the alcohol content is 80%, centrifuge, precipitate and freeze-dry to obtain the total polysaccharide, The product yield is more than 3%. The sugar content of the total polysaccharides of Houttuynia cordata measured by the sulfuric acid-phenol method exceeds 70%; the uronic acid content of the total polysaccharides of Houttuynia cordata measured by the m-hydroxybiphenyl method exceeds 25%; The protein content of total grass polysacchari...

Embodiment 2

[0057] Example 2: Drug efficacy test of Houttuynia cordata polysaccharide in alleviating ulcerative colitis

[0058] 2.1 Experimental materials

[0059] 2.1.1 Experimental animals and drugs

[0060] 24 C57BL / 6 male mice, weighing 16-18g, were purchased from Shanghai Lingchang Co., Ltd., animal certificate number: SCXK (Shanghai) 2013-0018. The experimental animals were housed in separate cages, the litter was changed every 3 days, and they were adaptively fed for 2 days. During the experiment, they had free access to water and food. The temperature of the feeding environment is 20±2°C, the relative humidity is 60%, and the cycle of 12 hours of light and 12 hours of darkness every day;

[0061] Houttuynia cordata polysaccharide, the total polysaccharide extracted from Houttuynia cordata by embodiment 1 (lot number JY2011); 2.1.2 Experimental reagents and instruments

[0062] Chemical reagents: DSS (dextran sodium sulfate, MW36000-50000, MP Biomedicals, LOT NO: Q6182), PBS bu...

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and relates to a novel pharmaceutical application of Houttuynia cordata Thunb. polysaccharide, in particular to the application of the Houttuynia cordata Thunb. polysaccharide in preparation of a medicine for preventing and treating inflammatory bowel disease (IBD). A total polysaccharide extract is separated and extracted from the traditional Chinese medicine Houttuynia cordata Thunb., the average product yield is 3.65%, and the polysaccharide content exceeds 70%. An integral experiment proves that the total polysaccharide extract of the Houttuynia cordata Thunb. has a remarkable treatment effect on mouse colonic injury induced by 2.5% DSS. The Houttuynia cordata Thunb. polysaccharide may inhibit infiltration of inflammatory cells, reduce secretion of inflammatory cytokines and inhibit expression of an inflammatory transcription factor NF [kappa] B through an anti-inflammatory mechanism of the Houttuynia cordata Thunb. polysaccharide, so that intestinal injury is reduced, intestinal mucosa is protected, and colon shortening and weight loss are relieved. The Houttuynia cordata Thunb. polysaccharide can be used for preparing medicines for preventing and treating ulcerative colitis.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and relates to the new pharmaceutical application of Houttuynia cordata polysaccharide, in particular to the application of Houttuynia cordata polysaccharide in preparing medicines for preventing and treating inflammatory bowel disease (IBD). Background technique [0002] The prior art discloses that inflammatory bowel disease is caused by various reasons such as genes, environment, and immunity. The common types of inflammatory bowel disease include ulcerative colitis (ucerative colitis, UC) and Crohn's disease (CD); wherein, ulcerative colitis mainly occurs in the mucosal layer of the rectum and colon, with ulcers, erosions Mainly, often involving the entire colon, showing continuous large-scale lesions[1], the clinical manifestations of UC are recurrent diarrhea, abdominal pain, nausea, vomiting, bloody stool, fever and malnutrition, which may be accompanied by skin, mucou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P29/00A61P1/00C08B37/00
CPCA61K31/715A61P29/00A61P1/00C08B37/0003C08B37/00
Inventor 朱海燕岑利峰陈道峰施晨晨卢燕史训龙力弘
Owner FUDAN UNIV
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