Fusion gene and its coded protein for stimulating body's anti-African swine fever infection and application

A technology that combines genes and African swine fever, applied in the field of genetic engineering, can solve the problems of strong virulence, low vaccine protection, and difficulty in overcoming, and achieve the effect of resisting damage

Active Publication Date: 2022-06-07
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Researchers at home and abroad have been working on the research and development of African swine fever virus vaccines, but the risks of low vaccine protection, strong virulence and loose virus have been difficult to overcome

Method used

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  • Fusion gene and its coded protein for stimulating body's anti-African swine fever infection and application
  • Fusion gene and its coded protein for stimulating body's anti-African swine fever infection and application
  • Fusion gene and its coded protein for stimulating body's anti-African swine fever infection and application

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Experimental program
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Effect test

Embodiment

[0122] 1. Identification of single-anchor expression plasmids pSIP409-pgsA'-P12-DNBLK-Ubb-P30(E) and pSIP409-pgsA'-F△D3-TCC-P12-DNBLK-Ubb-P30(E)

[0123] 1.1 Identification of primer synthesis and target fragment identification

[0124] Primers MD1 and MD2 were identified based on the optimized fusion DNA fragments P12-DNBLK-Ubb-P30 and FΔD3-TCC-P12-DNBLK-Ubb-P30.

[0125] Table 1-1 Primer sequences

[0126]

[0127] Note: italics are marked as restriction sites.

[0128] Plasmids pSIP409-pgsA'-P12-DNBLK-Ubb-P30 (E) and pSIP409-pgsA'-FΔD3-TCC-P12-DNBLK-Ubb-P30 (E) were miniprep. PrimeSTAR Max Premix (2×) high-fidelity enzyme from Takara company was used to amplify the target fragment by PCR, and it was identified by double digestion with XbaI and HindIII.

[0129] The PCR identification systems of plasmids pSIP409-pgsA'-P12-DNBLK-Ubb-P30(E) and pSIP409-pgsA'-F△D3-TCC-P12-DNBLK-Ubb-P30(E) are as follows:

[0130] Table 1-2 PCR system

[0131]

[0132]

[0133]Afte...

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Abstract

This application provides a fusion gene for stimulating a body to resist African swine fever infection, its encoded protein and its application. The fusion gene includes the resistance gene in the P30 gene, and at least one selected from the group consisting of the adhesion protein P12 gene, the microtubule dynein competition protein DNBLK gene and the ubiquitin protein Ubb gene. At the same time, the present invention also provides a recombinant plantarum lactobacillus capable of expressing the fusion gene, which can be well colonized in the intestinal tract of pigs, and successfully expresses the fusion proteins P12-DNBLK-Ubb-P30 and F△ with resistance to African swine fever infection D3‑TCC‑P12‑DNBLK‑Ubb‑P30 has a good immune effect and can be used to prevent and control African swine fever.

Description

technical field [0001] The present application relates to the technical field of genetic engineering, in particular to a fusion gene that stimulates the body to resist African swine fever infection and its encoded protein and application. Background technique [0002] The information disclosed in this Background of the Invention is intended to enhance understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information is already known as the prior art to a person of ordinary skill in the art. [0003] African swine fever (ASF) is a highly contagious swine viral disease with a mortality rate of nearly 100% in domestic pigs. African swine fever virus (ASFV), which causes hemorrhagic fever in domestic pigs, is the greatest global threat to the livestock industry on record. ASF is an acute, hemorrhagic, highly contagious infectious disease caused by a large double-stranded DNA virus, ...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/74C12N1/21A23K10/18A23K20/147A23K20/153A61K39/12A61P31/20C12R1/25
CPCC07K14/005C07K14/00C12N15/746A23K10/18A23K20/147A23K20/153A61K39/12A61P31/20C07K2319/00C12N2710/12022C12N2710/12033C12N2710/12034A61K2039/53A61K2039/523
Inventor王春凤杨桂连陈晓雷杨文涛牛天明石春卫包军付李楠王丹江雨鑫王红谷巍单宝龙
OwnerJILIN AGRICULTURAL UNIV