Aquaculture feed and preparation method thereof
A technology for aquaculture and feed, which is applied in the field of aquaculture feed and its preparation, can solve the problems of increased aquaculture waste and poor water quality, and achieve the effects of reasonable feed formula, improved synthesis capacity, and improved oxygen uptake capacity
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[0032] Wherein, the preparation method of the microbial fermented feed comprises: inoculating Cordyceps fungus and Schizochytrium fungus into the fermentation medium for fermentation culture, then performing solid-liquid separation on the fermentation culture product, and drying the separated solid to obtain The microorganisms ferment feed.
[0033] In the course of the research, the inventors found that using the microbial fermented feed obtained by the co-fermentation of Cordyceps and Schizochytrium, in the microbial fermentation process, the co-fermentation of Cordyceps and Schizochytrium can effectively improve the synthesis of oil in the fermentation process ability, thereby increasing the oil content in the fermented product. Moreover, it can provide a variety of biologically active substances during the co-fermentation process of Cordyceps and Schizochytrium, and can work together with bean cake, rapeseed cake, sweet potato flour, corn flour, sodium chloride, and vitami...
Embodiment 1
[0064](1) Inoculate Cordyceps militaris and Schizochytrium HX-308 into seed medium (yeast extract 8g / L, peptone 20g / L and D-glucose 20g / L, pH 6 ) in a shaking table (rotating speed 220rpm) for 6 days to obtain Cordyceps militaris seed liquid and Schizochytrium HX-308 seed liquid, and then above-mentioned Cordyceps militaris seed liquid (inoculum size 5%) and Schizochytrium HX-308 seed solution (inoculum size 5%) was inoculated into fermentation medium (yeast extract 8g / L, peptone 20g / L, D-glucose 20g / L, sodium glutamate 10g / L, sulfuric acid heptahydrate Magnesium 5g / L and potassium dihydrogen phosphate 7g / L, pH 6) in a shaker (rotational speed 220rpm) for 12 days; the fermentation product was subjected to solid-liquid separation, and then the separated solid was frozen at -40°C Dry for 24 hours, and make the particle size less than or equal to 0.2 μm by jet milling; obtain microbial fermented feed.
[0065] (2) Mix 3kg microbial fermented feed, 2kg bean cake, 1kg rapeseed cak...
Embodiment 2
[0067] (1) Inoculate Cordyceps militaris and Schizochytrium HX-308 into seed medium (yeast extract 8g / L, peptone 20g / L and D-glucose 20g / L, pH 6 ) in a shaking table (rotating speed 180rpm) for 4 days to obtain Cordyceps militaris seed liquid and Schizochytrium HX-308 seed liquid, and then above-mentioned Cordyceps militaris seed liquid (inoculum size 3%) and Schizochytrium HX-308 seed liquid (inoculum size 9%) was inoculated into fermentation medium (yeast extract 8g / L, peptone 20g / L, D-glucose 20g / L, sodium glutamate 10g / L, sulfuric acid heptahydrate Magnesium 5g / L and potassium dihydrogen phosphate 7g / L, pH 6) in a shaker (rotation speed 180rpm) for 16 days; the fermentation product was subjected to solid-liquid separation, and then the separated solid was frozen at -20°C Dry for 48 hours, and make the particle size less than or equal to 0.2 μm by jet milling; obtain microbial fermented feed.
[0068] (2) Mix 3kg microbial fermented feed, 1.5kg bean cake, 1.2kg rapeseed ca...
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