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Citrus callus and suspension cell culture method

A technology of callus and culture method, which is applied in the field of culture of citrus callus and suspension cells, can solve problems such as the decline of market competitiveness, and achieve the effect of good dispersion and stable properties

Pending Publication Date: 2022-02-11
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the rapid development of the planting area of ​​Shatangju, some production and market problems are also increasing, and the market competitiveness is declining year by year.

Method used

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  • Citrus callus and suspension cell culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] formula:

[0051] Sterilization solution: 20% (v / v) sodium hypochlorite.

[0052] Mother liquor A: 95g / L KNO 3 , 82.5g / L NH 4 NO 3 , 18.5g / L MgSO 4 ·7H 2 O, 7.5g / L KH 2 PO 4 , 1g / LK 2 HPO 4 , dissolved in deionized water and stored at 4°C.

[0053] Mother liquor B: 0.62g / L boric acid, 1.68g / L MnSO 4 ·H 2 O, 0.86g / L ZnSO 4 ·7H 2 O, 0.083g / L KI, 0.025g / L Na 2 MoO 4 2H 2 O, 0.0025g / L CuSO 4 ·5H 2 O, 0.0025g / L CoCl 2 ·6H 2 O, dissolved in deionized water and stored at 4°C.

[0054] Mother solution C: 10g / L inositol, 1g / L vitamin B 1 , 1g / L vitamin B 6 , 0.5g / L niacin, 0.2g / L glycine, dissolved in deionized water, stored at 4°C.

[0055] Mother liquor D: 29.33g / L CaCl 2 2H 2 O, dissolved in deionized water and stored at 4°C.

[0056] Mother liquor E: 7.45g / L Na 2 -EDTA, 5.57g / L FeSO 4 ·7H 2 O, dissolved in deionized water and stored at 4°C.

[0057] Mother liquor F: 150g / L KCl, 37g / L MgSO 4 ·7H 2 O, 15g / L KH 2 PO 4 , 2g / L K 2 HPO 4 , disso...

Embodiment 2

[0065] formula:

[0066] Mother liquor A: 90g / L KNO 3 , 80g / L NH 4 NO 3 , 16g / L MgSO 4 ·7H 2 O, 5g / L KH 2 PO 4 , 1.5g / LK 2 HPO 4 , dissolved in deionized water and stored at 4°C.

[0067] Mother liquor B: 0.6g / L boric acid, 1.75g / L MnSO 4 ·H 2 O, 0.7g / L ZnSO 4 ·7H 2 O, 0.073g / L KI, 0.035g / L Na 2 MoO 4 2H 2 O, 0.0035g / L CuSO 4 ·5H 2 O, 0.0035g / L CoCl 2 ·6H 2 O, dissolved in deionized water and stored at 4°C.

[0068] Mother solution C: 8g / L inositol, 1.5g / L vitamin B 1 , 1.5g / L vitamin B 6 , 0.25g / L niacin, 0.1g / L glycine, dissolved in deionized water, and stored at 4°C.

[0069] Mother liquor D: 29g / L CaCl 2 2H 2 O, dissolved in deionized water and stored at 4°C.

[0070] Mother liquor E: 7.25g / L Na 2 -EDTA, 5.47g / L FeSO 4 ·7H 2 O, dissolved in deionized water and stored at 4°C.

[0071] Mother liquor F: 140g / L KCl, 40g / L MgSO 4 ·7H 2 O, 17g / L KH 2 PO 4 , 3g / L K 2 HPO 4 , dissolved in deionized water and stored at 4°C.

[0072] MT solid medi...

Embodiment 3

[0076] formula:

[0077] Mother liquor A: 100g / L KNO 3 , 85g / L NH 4 NO 3 , 20g / L MgSO 4 ·7H 2 O, 10g / L KH 2 PO 4 , 0.5g / LK 2HPO 4 , dissolved in deionized water and stored at 4°C.

[0078] Mother liquor B: 0.64g / L boric acid, 1.6g / L MnSO 4 ·H 2 O, 1g / L ZnSO 4 ·7H 2 O, 0.093g / L KI, 0.015g / L Na 2 MoO 4 2H 2 O, 0.0015g / L CuSO 4 ·5H 2 O, 0.0015g / L CoCl 2 ·6H 2 O, dissolved in deionized water and stored at 4°C.

[0079] Mother solution C: 12g / L inositol, 0.5g / L vitamin B 1 , 0.5g / L vitamin B 6 , 0.75g / L niacin, 0.3g / L glycine, dissolved in deionized water, stored at 4°C.

[0080] Mother liquor D: 30g / L CaCl 2 2H 2 O, dissolved in deionized water and stored at 4°C.

[0081] Mother liquor E: 7.65g / L Na 2 -EDTA, 5.67g / L FeSO 4 ·7H 2 O, dissolved in deionized water and stored at 4°C.

[0082] Mother liquor F: 160g / L KCl, 35g / L MgSO 4 ·7H 2 O, 10g / L KH 2 PO 4 , 1.5g / L K 2 HPO 4 , dissolved in deionized water and stored at 4°C.

[0083] MT solid medium...

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Abstract

The invention discloses a citrus callus and suspension cell culture method. The culture method comprises the following steps: S1, selecting seeds, performing disinfection, and taking out ovules; S2, inoculating the sterile ovules into a callus induction culture medium, and carrying out callus induction and subculture, so as to obtain calluses taking citrus as a raw material; S3, inoculating the callus into a corresponding liquid culture medium, and carrying out shake culture to obtain suspension cells with stable characters. According to the invention, the obtained calluses are stable in characters and high in induction rate, the high-quality calluses can be obtained, the obtained suspension cell particles are good in dispersity, and the solution is clear and transparent.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of plant seedlings, and in particular relates to a method for culturing citrus callus and suspension cells. Background technique [0002] Citrus is one of the most important fruits in the world, and my country is also an important place of origin, and many excellent varieties currently originate from my country. my country's citrus industry has made considerable progress so far, and its output ranks third in the world, but there are still many problems. Many diseases of citrus are still the main factors threatening the citrus industry, and disease-resistant breeding is regarded as the fundamental way to solve the disease problems. Therefore, it is a feasible way to study the interaction mechanism between pathogens and hosts, especially the host resistance response mechanism, to mine resistance / resistance-related genes, and to use disease-resistant genes for pyramiding tolerance / resistan...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/005
Inventor 吕远达姜波闫化学钟云
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI