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Application of passion fruit tissue culture medium

A technology of tissue culture medium and passion fruit, which is applied in application, plant regeneration, horticulture, etc., can solve the problems of low grafting yield, easy virus infection of cutting seedlings, limited production of passion fruit seedlings, etc., and achieve the effect of facilitating mass production

Inactive Publication Date: 2022-02-11
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] On the whole, passion fruit not only has high economic value, but also has great potential for development and utilization. However, in the production of passion fruit, it is usually cultivated and planted by cuttings, grafting and seed propagation, and cuttings are susceptible to viruses. , low grafting output, slow speed and other shortcomings, along with the improvement of passion fruit market demand, the production of passion fruit seedlings of the above method is limited, it is difficult to meet the commercial demand

Method used

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  • Application of passion fruit tissue culture medium
  • Application of passion fruit tissue culture medium
  • Application of passion fruit tissue culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: a kind of application of passion fruit tissue culture medium, implementation steps are as follows:

[0038] S1, the preparation of tissue culture medium: take MS medium as the basic medium, add agar powder, white granulated sugar and plant growth regulator to the basic medium to obtain the primary culture medium, subculture medium and / or rooting medium, The primary medium is made into a slant to facilitate the insertion of tissue culture materials and the spinner bottle before the subculture stage, and the subculture medium is a flat medium.

[0039] The tissue culture medium includes the primary culture medium, the subculture medium and the rooting medium; the pH of the primary medium, the subculture medium and the rooting medium are all 6.

[0040] The primary medium uses MS medium as the basic medium, and the components of the primary medium also include 6-BA 0.1-0.5mg / L, NAA 0.04mg / L, agar powder 5g / L and white sugar 25g / L;

[0041] The subculture med...

Embodiment 2

[0046] Embodiment 2: The difference from Example 1 is that the first-generation medium is based on MS medium, and the components of the first-generation medium also include 6-BA 0.3mg / L, NAA0.03mg / L, and agar powder 5g / L and white sugar 25g / L.

Embodiment 3

[0047] Embodiment 3: The difference from Example 1 is that the primary culture medium uses MS medium as the basic medium, and the components of the primary culture medium also include 6-BA 0.3mg / L, NAA0.01mg / L, and agar powder 5g / L and white sugar 25g / L.

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Abstract

The invention discloses an application of a passion fruit tissue culture medium, and belongs to the technical field of plant culture. The application is characterized in that the tissue culture medium comprises a primary culture medium, a subculture medium and a rooting culture medium; the pH values of the primary culture medium, the subculture medium and the rooting culture medium are 5.5-6.5; the primary culture medium takes an MS culture medium as a basic culture medium, and the components of the primary culture medium further comprise 0.1-0.5 mg / L of 6-BA, 0.01-0.04 mg / L of NAA, 5g / L of agar powder and 25g / L of white granulated sugar; the subculture medium takes an MS culture medium as a basic culture medium, and the subculture medium further comprises a growth regulator I, 0.03-0.05 mg / L of NAA, 5g / L of agar powder and 25g / L of white granulated sugar; the growth regulator I is 0.3 mg / L of LFS or 0.3 mg / L of 6-BA; and the rooting culture medium takes an MS culture medium as a basic culture medium, and the rooting culture medium further comprises 0.3 mg / L of IBA, 0.2 mg / L of NAA, 5g / L of agar powder and 25g / L of white granulated sugar. The application is mainly used in the production of the passion fruit tissue culture seedlings, has the advantages of rapidness, high quality and high yield, and is convenient for batch production.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to the application of a passion fruit tissue culture medium. Background technique [0002] The edible value of passion fruit is mainly reflected in the wonderful taste and nutritional value far higher than other fruits. The fruit juice tastes sour and sweet, the color is golden and translucent, and the taste is refreshing and not greasy. It is a very popular fruit juice drink. Studies have found that there are 17 kinds of amino acids in passion fruit, and the content of essential amino acids is as high as 13.30% of the total amino acids. The content of glutamic acid in passion fruit is particularly rich. Glutamic acid promotes the synthesis of red blood cells, can supply brain nutrition, activate thinking, improve memory, and is also effective for treating neurasthenia. Passion fruit also contains seven essential amino acids, leucine, methionine, lysine, valine, phenylala...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 周凤珏龚银花申雨菲梁琼月许皓翔许鸿源
Owner GUANGXI UNIV