Application of passion fruit tissue culture medium
A technology of tissue culture medium and passion fruit, which is applied in application, plant regeneration, horticulture, etc., can solve the problems of low grafting yield, easy virus infection of cutting seedlings, limited production of passion fruit seedlings, etc., and achieve the effect of facilitating mass production
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Embodiment 1
[0037] Embodiment 1: a kind of application of passion fruit tissue culture medium, implementation steps are as follows:
[0038] S1, the preparation of tissue culture medium: take MS medium as the basic medium, add agar powder, white granulated sugar and plant growth regulator to the basic medium to obtain the primary culture medium, subculture medium and / or rooting medium, The primary medium is made into a slant to facilitate the insertion of tissue culture materials and the spinner bottle before the subculture stage, and the subculture medium is a flat medium.
[0039] The tissue culture medium includes the primary culture medium, the subculture medium and the rooting medium; the pH of the primary medium, the subculture medium and the rooting medium are all 6.
[0040] The primary medium uses MS medium as the basic medium, and the components of the primary medium also include 6-BA 0.1-0.5mg / L, NAA 0.04mg / L, agar powder 5g / L and white sugar 25g / L;
[0041] The subculture med...
Embodiment 2
[0046] Embodiment 2: The difference from Example 1 is that the first-generation medium is based on MS medium, and the components of the first-generation medium also include 6-BA 0.3mg / L, NAA0.03mg / L, and agar powder 5g / L and white sugar 25g / L.
Embodiment 3
[0047] Embodiment 3: The difference from Example 1 is that the primary culture medium uses MS medium as the basic medium, and the components of the primary culture medium also include 6-BA 0.3mg / L, NAA0.01mg / L, and agar powder 5g / L and white sugar 25g / L.
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