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Chemical degradation method of dendrobium officinale polysaccharide

A technology for chemical degradation of Dendrobium officinale polysaccharide is applied in the field of chemical degradation of Dendrobium officinale polysaccharide, which can solve the problems of long reaction time and high required concentration, and achieve the effects of short degradation time, improved antioxidant activity and enhanced stability.

Active Publication Date: 2022-02-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The free radical degradation method of polysaccharides invented by Qu Aiqin et al. (Patent No.: CN1459455A) is to place polysaccharides in a complex system of organic acids, hydrogen peroxide and vitamin C, which is suitable for most sources of polysaccharides, but the reaction time For 2-72 hours, the concentration of the solution is in the range of 1-100mmol / L, and the concentration required for a long reaction time is high

Method used

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  • Chemical degradation method of dendrobium officinale polysaccharide
  • Chemical degradation method of dendrobium officinale polysaccharide
  • Chemical degradation method of dendrobium officinale polysaccharide

Examples

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Embodiment 1

[0043] The present embodiment provides a kind of chemical degradation method of dendrobium officinale polysaccharide, and described method comprises:

[0044] a) prepare Dendrobium officinale polysaccharide DP according to the method of comparative example;

[0045] b) using diethylenetriaminepentaacetic acid (DTPA) solution to chelate ferrous ions to obtain diethylenetriaminepentaacetic acid chelated ferrous ions, i.e. DTPA-ferrous ions;

[0046] c) Prepare the Dendrobium officinale crude polysaccharide DP prepared in step a) into an aqueous solution of Dendrobium candidum polysaccharides with a concentration of 2 mg / mL, and adjust the pH to 3.0; then add DTPA-ferrous ions to the DTPA-ferrous ions in the Dendrobium officinale polysaccharide aqueous solution The concentration is 5 μm / L; then add 1% hydrogen peroxide solution until the concentration of hydrogen peroxide in the Dendrobium officinale polysaccharide aqueous solution is 0.1%; carry out oxidation reaction at room te...

Embodiment 2

[0052] This example provides a chemical degradation method of Dendrobium officinale polysaccharide, which is basically the same as in Example 1, except that in step d), the reaction time is 10 minutes; the degradation product of Dendrobium officinale polysaccharide DP-2 is obtained.

Embodiment 3

[0054] This embodiment provides a chemical degradation method of Dendrobium officinale polysaccharide, which is basically the same as in Example 1, the only difference being that in step c), the DTPA-ferrous ion is replaced by ferrous ion: adding ferrous ion, To the concentration of ferrous ions in the Dendrobium officinale polysaccharide aqueous solution is 5 μm / L. Finally, the polysaccharide degradation product DP-3 of Dendrobium officinale was obtained.

[0055] figure 1 It is the high performance liquid chromatogram of Dendrobium officinale polysaccharide DP and Dendrobium officinale degraded polysaccharide DP-1, DP-2 and DP-3, by figure 1 It can be seen that the molecular weights of DP, DP-1, DP-2, and DP-3 were analyzed by high performance liquid chromatography, and it was determined that DP is mainly composed of polysaccharides with four molecular weights, which are 2.4×10 7 Da, 1.9×10 6 Da, 1.3×10 6 Da, 1.5×10 5 Da; DP-1 is mainly composed of two molecular weight ...

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Abstract

The invention belongs to the field of polysaccharide preparation, and particularly relates to a chemical degradation method of dendrobium officinale polysaccharide. Diethylenetriamine pentaacetic acid chelated ferrous ions and hydrogen peroxide react to form a Fenton system, and produced hydroxyl free radicals oxidize and degrade dendrobium officinale polysaccharides. The degradation method provided by the invention is simple in process, the molecular weight of the dendrobium officinale polysaccharide can be effectively reduced after the dendrobium officinale polysaccharide is treated by the method, and subsequent utilization of the dendrobium officinale polysaccharide is facilitated.

Description

technical field [0001] The invention belongs to the field of polysaccharide preparation, and in particular relates to a chemical degradation method of dendrobium officinale polysaccharide. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale) is a plant of the genus Dendrobium orchidaceae. Its leaves are two rows of oblong-lanceolate, and the stems are not branched and have many nodes. Dendrobium candidum is often used as medicine with fresh or dried stems. It has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting the stomach, moistening the lungs and relieving cough, improving eyesight and strengthening the body. It is a traditional precious Chinese medicinal material. [0003] Dendrobium officinale polysaccharide is the main active ingredient in Dendrobium officinale, and its content ranges from 10-49%. It is a water-soluble heteropolysaccharide, mostly composed of α, β-(1→4) glycosidic bonds linkin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/006
Inventor 张秀清孟继坤葛鑫会张楠
Owner CHINA AGRI UNIV