Kit for early diagnosis of Alzheimer's disease and application thereof

A technology for early diagnosis of Alzheimer's disease, applied in disease diagnosis, biological testing, material inspection products, etc., can solve the problems of lack of accurate prediction ability of Alzheimer's disease, long detection time, complicated operation, etc., and achieve easy The effect of operation, wide detection range and simple operation

Pending Publication Date: 2022-02-11
SHANDONG UNIV QILU HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a kit for early diagnosis of Alzheimer's disease and its application. In the present invention, magnetic particles and chemiluminescent technology are combined to solve the existing Alzheimer's disease. Alzheimer's disease detection kits are complicated to operate, expensive, long detection time, low sensitivity, poor precision, lack of accurate prediction ability for Alzheimer's disease, etc., for large-scale early screening of Alzheimer's disease Has important application value and significance

Method used

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  • Kit for early diagnosis of Alzheimer's disease and application thereof
  • Kit for early diagnosis of Alzheimer's disease and application thereof
  • Kit for early diagnosis of Alzheimer's disease and application thereof

Examples

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preparation example Construction

[0030] In one embodiment of the present invention, a kind of preparation method of above-mentioned kit is provided, and described preparation method comprises:

[0031] The preparation method of reagent A is as follows: T-Tau, p-Tau-181, p-Tau-217 and p-Tau-231 monoclonal antibodies are mixed with biotin at a molar ratio of 1:10-1:40 Mix well at room temperature and react for 1-4 h, then dialyze the reactant in PBS buffer to obtain biotin-labeled monoclonal antibody; add biotin-labeled monoclonal antibody to the buffer of streptavidin magnetic microspheres In the system, the coupling ratio of biotinylated protein to streptavidin magnetic microspheres is 10-30μg:1mg.

[0032] The preparation method of reagent B is as follows: mix the monoclonal antibody of Tau protein N 1-17 site and acridinium ester according to the molar ratio of 1:10-1:40, react at room temperature for 2-4 h, and then add lysine The coupling reaction was terminated by acid, and the reaction product was obta...

Embodiment 1

[0041] In this embodiment, a kit for early diagnosis of Alzheimer's disease is provided, which includes: reagent A1 (T-Tau magnetic microsphere working solution), A2 (p-Tau-181 magnetic microsphere Ball working solution), A3 (p-Tau-231 magnetic microsphere working solution), A4 (p-Tau-217 four kinds of magnetic microsphere working solution), reagent B1 (monoclonal Antibody-prepared acridinium ester working solution), calibrator (universal composite calibrator, used to establish a standard curve).

[0042] Step 1: Buffer configuration

[0043] 1. The preparation method of the buffer system of magnetic microspheres

[0044] In the 0.15 M PBS buffer system, adjust the pH to 7.3, add 3% BSA, 2% trehalose, 0.05% magnetic microsphere dispersant, 0.25% Tween 20, 0.05% Proclin 300, mix well until completely dissolved, Add an appropriate amount of purified water to quantify, stir and mix for 1 h, and adjust the pH to 7.3. Filter it with a filter device and store it at 4°C for future...

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Abstract

The invention relates to the technical field of biological detection, in particular to a kit for early diagnosis of Alzheimer's disease and application of the kit. According to the kit, the purpose of early diagnosis of the Alzheimer's disease is achieved through combined detection of T-tau, p-Tau-181, p-Tau-217 and p-Tau-231 in serum and plasma. The kit is wide in detection range, high in sensitivity and good in precision, is suitable for a full-automatic chemiluminiscence instrument, is simple to operate, is suitable for the requirement of clinically detecting a large amount of serum and plasma, and provides more accurate and more personalized guidance opinions for early diagnosis of Alzheimer's disease.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit for early diagnosis of Alzheimer's disease and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Alzheimer's Disease (AD), also known as primary senile dementia, is a fatal neurodegenerative disease and belongs to the most common type of dementia, accounting for about 50% to 70% of dementias. Dementia-like; its main symptoms are memory decline, cognitive decline, behavioral decline, and decreased living ability, etc., and may also cause depression, anxiety and other complications, which are very harmful. Currently, the main diagnostic methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/543
CPCG01N33/6896G01N33/577G01N33/54326G01N2800/2821G01N2333/4709
Inventor 王谦王静赵凤高洪琪张春杰胡晓明杨小蕾
Owner SHANDONG UNIV QILU HOSPITAL
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