Preserving fluid and application thereof

Abstract

The invention discloses a preserving fluid. The preserving fluid is prepared from one or a mixture of more than two of 5-aminolevulinic acid, L-carnosine, L-glutamic acid and D-biotin. The invention also discloses an application of the preserving fluid. Compared with the prior art, the 5-aminolevulinic acid, the L-carnosine, the L-glutamic acid and the D-biotin are added into the preserving fluid, cells, tissues and / or organs are subjected to cold preservation, the cell survival rate is high, cell apoptosis caused by cold preservation-rewarming can be effectively reduced, mitochondrial membrane potential can be stabilized, mitochondria can be prevented from excessively generating reactive oxygen free radicals, mitochondrial injury caused by cold preservation-rewarming can be effectively reduced, accordingly, ischemia reperfusion injury of donation tissues and organs in transplantation procedures can be reduced, and the preserving fluid has great significance in progress of organ transplantation preservation technologies.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to a preservation solution for keeping cells alive without blood supply, reducing in vitro preservation damage, and promoting the recovery of tissues and organs, and the application of the preservation solution. Background technique [0002] As early as the 20th century, there were the first organ transplants. Organ transplantation is to place a healthy organ into a critically ill patient through surgery or other methods, so that the organ can continue to function, so that the organ recipient can be reborn. For resected healthy organs, it is necessary to perfuse and impregnate them with preservation solution, so as to prevent organ necrosis and ischemia-reperfusion injury. The so-called ischemia-reperfusion refers to the reperfusion of an organ or tissue in an ischemic state, causing various toxic substances to be produced in the microcirculation of the organ or tissue, thereby cau...

AI Technical Summary

Problems solved by technology

[0003] Although the existing preservation solutions can help organs prevent cell swelling and reduce ROS generation during low-temperature storage, and have a certain effect on reducing ischemic damage and promoting organ recovery, they still have no effect on reducing ischemia-reperfusion injury. Satisfactory results are achieved, limiting the amount of time an organ can be safely kept outside the body and potentially increasing the risk of organ damage during transplant surgery

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