Special peptone for producing inosine through fermentation as well as preparation method and application of peptone

A technology of peptone and inosine, which is applied in the field of peptone preparation, can solve the problems of high production cost affecting the large-scale production of inosine, different effects of inosine fermentation level, and high requirements for equipment conditions, so as to prolong the synthesis time of inosine and improve the production efficiency of inosine. Yield, the effect of promoting growth and reproduction

Pending Publication Date: 2022-02-18
淄博锦源生物科技股份有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of inosine by chemical synthesis requires high equipment conditions, complex process, and limited production; the high production cost of enzymatic hydrolysis affects the large-scale production of inosine; microbial fermentation is currently the main method used to produce inosine
[0004] Peptone is the main raw material of microbial culture m

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Special peptone for producing inosine through fermentation as well as preparation method and application of peptone

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1

[0029] Method for the preparation of biocyramine for fermentation, including the following steps:

[0030] (1) Take fresh fish bone, add water high temperature and high pressure cooking (fresh fish bone and water mass ratio of 1: 6), under 3 standard atmospheric pressure, heat insulation for 1 hour. Bone soup is pressed to the storage tank (in the can, the oil separator will remove the bone soup of the oil layer to the enzymatic canister, adjust the pH to 7, temperature 50 ° C, add a total of 1 ‰ of the total fish bone Protease, 2 ‰ of aminogenase, 1 ‰ of neutral protease, slowly stir, stir for 5 minutes, stir 10 min to stop for 5 min, stir for 15 minutes to stop 10 min, stir 15 min to stop 15 min. End of the enzymatic solution, add 500 mL of acetic acid, then tonify pH to 5.1 with hydrochloride, and slowly cool down after heating to 90 ° C, 2-3 hours fall to about 50 ° C, and the mixing process cannot be stirred during cooling. After the end, the filtration was...

Example Embodiment

[0034] Example 2

[0035](1) Take fresh fish bone, add water high temperature and high pressure cooking (fresh fish bone and water mass ratio of 1: 6), under 3 standard atmospheric pressure, heat insulation for 1 hour. The bone soup is pressed to the storage tank (in the cans of itself). After the oil-water separator, the bone soup of the oil layer will be removed to the enzymatic canister, adjust the pH to 7.2, temperature 50 ° C, add the total amount of fish bone. Protease, 2 ‰ of aminogenase, 1 ‰ of neutral protease, slowly stir, stir for 5 minutes, stir 10 min to stop for 5 min, stir for 15 minutes to stop 10 min, stir 15 min to stop 15 min. End of the enzymatic solution, add 500 ml of acetic acid, then tonify pH to 5.2 with hydrochloride, and slowly cool down after heating to 90 ° C, 2-3 hours fall to about 50 ° C, and the mixing process cannot be stirred during cooling. After the end, the filtration was filtered (400 mesh filter cloth), the obtained filtrate was concentrated...

Example Embodiment

[0039] Example 3

[0040] (1) Take fresh fish bone, add water high temperature and high pressure cooking (fresh fish bone and water mass ratio of 1: 6), under 3 standard atmospheric pressure, heat insulation for 1 hour. Bone soup is pressed to the storage tank (itself pressure in cannas) Protease, 2 ‰ of aminogenase, 1 ‰ of neutral protease, slowly stir, stir for 5 minutes, stir 10 min to stop for 5 min, stir for 15 minutes to stop 10 min, stir 15 min to stop 15 min. The end of the enzymatic solution, add 500 ml of acetic acid, then tonify pH to 5.3 with hydrochloride, slowly cool down after heating to 90 ° C, 2-3 hours fall to about 50 ° C, can not be stirred during cooling. After the end, the filtration was filtered (400 mesh filter cloth), the obtained filtrate was concentrated in vacuo, concentrated to 50% solid content, i.e., the fish bone protein concentrate.

[0041] (2) Take a fresh cow bone, add high temperature and high pressure cooking (1: 6 of fresh bovine bones and wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses special peptone for producing inosine through fermentation as well as a preparation method and application of the peptone, belonging to the field of peptone preparation. The preparation method for special peptone used for producing inosine through fermentation comprises the following steps: (1) adding various enzymes into fishbone soup to obtain a concentrated fishbone peptone solution; (2) adding various enzymes into a bovine bone soup to obtain a concentrated bovine bone peptone solution; (3) adding various enzymes into a soybean meal filtrate to obtain a concentrated soybean meal peptone solution; and (4) mixing the above three concentrated solutions in proportion, and conducting drying into powder to obtain the special peptone for fermentation production of inosine. The peptone prepared by the method is reasonable in molecular weight distribution, effectively controls a strain fermentation metabolism speed while promoting mass propagation and growth of strains, prolongs inosine synthesis time, increases inosine yield, and lays an important foundation for large-scale inosine fermentation production.

Description

technical field [0001] The invention relates to the field of peptone preparation, in particular to a special peptone for fermentation production of inosine and its preparation method and application. Background technique [0002] Inosine, also known as inosine, has the molecular formula C 10 h 12 N 4 o 5 , the relative molecular mass is 268.23. Inosine has a very wide application value in the fields of food and medicine. In the field of food, inosine is a delicious taste in food, and its content is often used as one of the characteristic indicators to measure the "freshness" of food; it is a variety of Nutritional freshness aids for seasoning foods such as monosodium glutamate, soy sauce, etc., and also one of the main raw materials for effective freshness enhancers inosinic acid and taste nucleotide disodium in the food industry; in the field of medicine, inosine is a cell Rejuvenating agent, has functions such as stimulating cells and enhancing cell metabolism. It is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P21/06C12P19/40
CPCC12P21/06C12P19/40
Inventor 李怀涛李怀深张学艳
Owner 淄博锦源生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap