Donkey spleen protein iron-supplementing oral liquid and preparation method thereof
A technology of oral liquid and protein solution, which is applied in the direction of protein food components, protein components of waste, functions of food components, etc., can solve the problems of human side effects, effects, unstable properties, etc., and achieve low equipment cost and good repeatability , Increase the effect of application value
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Embodiment 1
[0028] A donkey spleen protein iron supplement oral liquid, the preparation method is as follows:
[0029] (1) Take a donkey spleen with a net weight of about 500g, remove the fat tissue outside the spleen, cut it into small pieces, add deionized water at a ratio of 1.5mL water / tissue (g), break it with a homogenizer, and stir until No mass tissue.
[0030] (2) Filter the above non-agglomerated tissue with gauze, collect the filtrate and homogenize every 200 mL for 2 min to obtain a homogenized liquid.
[0031] (3) Boil the above homogeneous solution for 10 minutes, centrifuge at 8000g for 30 minutes at 4°C, discard the precipitate, and take the supernatant.
[0032] (4) Add 50% magnesium chloride to the above supernatant for salting out, let it stand overnight at 4°C, centrifuge the sample at 8000g for 30min the next day, discard the supernatant, redissolve the precipitate with aqueous solution, and dialyze to remove salt to obtain donkey spleen protein extraction liquid. ...
Embodiment 2
[0035] An oral solution for supplementing iron with donkey spleen protein, the preparation method is the same as in Example 1, the difference is that the solution used for homogenization is 500 mL, and the homogenization time is 2 minutes.
Embodiment 3
[0037] An oral solution for supplementing iron with donkey spleen protein. The preparation method is the same as that in Example 1, except that the homogeneous solution is boiled for 5 minutes, centrifuged at 5000 g for 30 minutes at 4° C., the precipitate is discarded, and the supernatant is taken.
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