Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for inhibiting generation of rice yeast acid and application

A rice yeast acid and acetic acid technology, which is applied in the application, food ingredients, food science and other directions to achieve the effect of preventing rice yeast acidosis and low cost

Active Publication Date: 2022-03-04
SOUTH CHINA AGRI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this study was carried out on a medium suitable for the growth of pathogenic variants of Burkholderia gladiolus, which is quite different from the specific edible fungi such as white fungus and fungus in real life.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inhibiting generation of rice yeast acid and application
  • Method for inhibiting generation of rice yeast acid and application
  • Method for inhibiting generation of rice yeast acid and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Purification of Burkholderia gladiolus Pseudomonas cocotoxin pathogenic strain

[0030] Streak on the potato dextrose agar (cocotoxin) medium (PDA) plate of the gladiolus Burkholder pv. cocotoxin (ATCC number 33664) carrying the oryzae acid-producing strain and culture it at 37° C. for 24 hours.

[0031] Pick a single colony and purify and culture it on a PDA plate for 24 hours again. Scrape an appropriate amount of colony with an inoculation loop and add it to a 3 mL sterile saline test tube to prepare a bacterial suspension with a McFarland (MCF) concentration of 1 (about 10 8 cfu / mL).

[0032] Choose a density of 10 8 The cfu / mL bacterial solution was used as the seed solution, and the seed solution was placed in a 4°C refrigerator for later use.

Embodiment 2

[0033] Example 2 Effects of Sodium Chloride on the Secretion of Oryzanic Acid by Burkholderia Gladioli

[0034] 1. Experimental method

[0035] (1) Inoculation of Burkholderia gladiolus pathogenic variant of cocotoxin

[0036] Aseptic fresh white fungus and fungus were used as food substrates for the growth of Burkholderia gladiolus pv. cocotoxin.

[0037] The seed liquid prepared in example 1 is diluted with physiological saline to contain 10 7 cfu / mL bacterial suspension. Take 1mL of the bacterial suspension and inoculate it into a homogeneous bag containing 5g of food matrix, add 100mL of sterile water containing different concentrations of sodium chloride to the homogeneous bag to soak the food matrix, the mass percentage concentration of sodium chloride is 0 %, 0.5%, 1.0%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0%, each concentration set 3 replicates. Incubate at 26°C for 48h.

[0038] After culturing for 48h, take 1.0mL solution sample from the homogeneous bag, dilute it with no...

Embodiment 3

[0046] Example 3 Effect of pH on the secretion of oryzae acid by Burkholderia gladiolus P. cocos

[0047] 1. Experimental method

[0048] (1) Inoculation of Burkholderia gladiolus pathogenic variant of cocotoxin

[0049] Fresh white fungus and fungus were used as food substrates for the growth of Burkholderia gladiolus pathogenic variety cocotoxin.

[0050] The seed liquid prepared in example 1 is diluted with physiological saline to contain 10 7 cfu / mL bacterial suspension. Take 1mL of the bacterial suspension and inoculate it into a homogeneous bag filled with 5g of food matrix. Use acetic acid and baking soda to adjust the pH of sterile water to 3, 4, 5, 6, 7, 7.4, 7.6, 7.8, 8, 8.2. The concentrations of acetic acid and baking soda in sterile water with different pH are shown in Table 1.

[0051] Table 1 Concentrations of acetic acid and baking soda in sterile water with different pH

[0052]

[0053] Add 100 mL of sterile water with different pH values ​​to soak th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Colony countaaaaaaaaaa
Colony countaaaaaaaaaa
Colony countaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for reducing rice yeast acid and application. The method comprises the step of adding one or more of sodium chloride, baking soda and / or acetic acid into an aqueous solution for soaking the edible mushrooms. The method for reducing the rice yeast acid is safe, simple, low in cost and suitable for daily life, in the process of treating edible mushrooms, generation of the rice yeast acid by the Burkholderia gladioli coconut poison pathogenic variants can be remarkably inhibited, all needed materials can be taken from kitchen seasonings, and the method can be widely applied to prevention of rice yeast acid poisoning in daily life.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a method and application for inhibiting the production of rice yeast acid. Background technique [0002] Burkholderia gladioli pv. Cocovenenans (BGC) is a Gram-negative bacterium isolated from fermented food in Indonesia. It is easy to contaminate fermented cornmeal, glutinous corn glutinous rice balls and other cereal products and deteriorate Tremella. Burkholderia gladiolus pv. cocotoxin is the only pathogenic strain of Burkholderia gladiolus that can cause food poisoning in humans. The secondary metabolite produced by this bacterium is Bongkrekic acid (Bongkrekic acid, BA) is a strong respiratory toxin that harms the liver, brain, kidneys and other organs. Human ingestion of rice yeast acid will lead to poisoning, and severe cases will die due to respiratory failure. Due to the strong acid and heat stability of rice yeast, ordinary heating cooking methods cannot remove it at all. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L31/00A23L29/00
CPCA23L31/00A23L29/015A23L29/035A23V2002/00A23V2250/1582A23V2250/1614A23V2250/1576A23V2250/022Y02E50/10
Inventor 徐振林曹雪铭王宇雷红涛孙远明罗林王弘沈玉栋
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com