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Waxy bacillus and its prepn

A technology for Bacillus cereus and spores, which is applied in the field of Bacillus cereus and its preparation, can solve problems such as inability to significantly improve insecticidal activity, and achieve the effects of obvious synergistic effect, improved insecticidal activity and low cost

Inactive Publication Date: 2004-03-10
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All these synergistic factors can improve the control effect of Bt preparations on target pests to a certain extent, but they cannot significantly improve their insecticidal activity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Separation and cultivation of strains: a little soil sample collected is put into the nutrient culture solution test tube that 4.5ml is housed, and the nutrient culture solution is peptone 1% beef extract 0.3%NaCl 0.5%PH7.5, through 76 ℃ , after 15 minutes in water bath, shake culture at 30°C for 51 hours. Dilute the culture medium to 10 -8 times, spread it on the separation medium, the separation medium is peptone 1% beef extract 0.3% NaCl 0.5% agar 2% PH7.5, cultured upside down in a 30°C incubator for 3 days, microscopically selected to produce spores but no paraspora A single colony of crystals was obtained, and a Bacillus cereus strain Ym-2102 was obtained. Purify its single colony on LB medium, LB medium is NaCl 1%, peptone 1%, yeast powder 0.5%, agar 1.5%, pH7.0, then inoculate in fermentation medium for shake flask fermentation, fermentation medium 2% starch, 4% soybean powder, 0.5% yeast powder, 0.5% peptone, KH 2 PO 4 0.1%, K 2 HPO 4 0.2%...

Embodiment 2

[0018] Bacterial strain isolation and cultivation: Put a little of the collected soil sample into a test tube containing 4.5ml of nutrient culture solution, and culture it with shaking for 48 hours at 78°C in a water bath for 12 minutes. Dilute the culture medium to 10 -9 times, spread it on the separation medium, culture it upside down in a 29°C incubator for 3 days, and select a single colony that produced spores but no parasporulation crystals by microscopic examination, and obtained a Bacillus cereus strain Ym-2102. Purify its single colony on LB medium, inoculate it on the fermentation medium for shake flask fermentation: scrape a ring of bacteria from the separation medium, add it to a test tube with 5ml of distilled water and shake well, bathe in 76°C water for 12 minutes, take out Cool to room temperature; take 1ml of the above-mentioned synchronized bacterial suspension and inoculate it into a 500ml large Erlenmeyer flask containing 40ml of culture medium. The Bacill...

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Abstract

The present invention is bacillus cereus and its preparation. The preparation of Bacillus cereus (Bc) YM-2102, CCTCC No.M202011, includes water bath vibrating culture of soil sample in nutritious liquid test tube at 75-78 deg.c for a period, diluting the culture liquid, spraying the culture liquid to LB culture soil, inverse culture in a thermostat for a period, microscope inspection of colony, purification of single colony in LB culture medium, inoculation to fermentation culture medium for shaking culture until spore falling-off. The said process is simple, easy to operate, low in cost, can raise the pesticidal activity of bacillus thuringiensis pesticide and is suitable for industrial production.

Description

technical field [0001] The present invention relates to the screening of synergists of Bacillus thuringiensis (Bt) biopesticides, in particular to a Bacillus cereus (Bc) capable of synergizing Bt, and to a preparation method of the bacterium . technical background [0002] Bacillus thuringiensis (Bt) preparations are currently the most widely used and most effective microbial insecticides. Belonging to Gram-positive bacteria, its toxic effect is mainly derived from the parasporal crystals produced during sporulation. It became commercially available in France for the first time in 1938. Since commercialization, Bt products have been favored for their high efficiency, safety, and specificity to target pests, and have gradually become the most deeply researched and most widely used biopesticides. Although Bt has many advantages, it also has problems such as unsatisfactory insecticidal effect and insensitivity to some target pests when it is applied...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 袁志明刘铭蔡全信刘海舟阎建平
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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