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Beauveria bassiana USCM6 and fungicide, application of beauveria bassiana USCM6 and method for preventing and treating pests

A technology for controlling pests of Beauveria bassiana, applied in the field of microorganisms, can solve problems such as ineffective control effects, pesticide residues, and potential safety hazards for humans and animals, and achieve excellent control effects, less resistance, and wide application prospects

Active Publication Date: 2022-03-11
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Currently, chemical control methods are mainly used for the prevention and control of this insect. Due to the large dose of pesticide used by farmers, the insect has a strong resistance to pesticides, resulting in pesticide residues, and The control effect is not obvious, and it is a hidden danger to the safety of humans and animals. Therefore, the current control of chemical pesticides is subject to certain restrictions (Lu Hui, Tang Jihong, Lu Baogan, Ma Zilong, He Xing, Chen Qi, Su Hao. Biological control and potential invasion of Spodoptera frugiperda Risk[J].Journal of Tropical Crops,2019,40(06):1237-1244.)

Method used

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  • Beauveria bassiana USCM6 and fungicide, application of beauveria bassiana USCM6 and method for preventing and treating pests
  • Beauveria bassiana USCM6 and fungicide, application of beauveria bassiana USCM6 and method for preventing and treating pests
  • Beauveria bassiana USCM6 and fungicide, application of beauveria bassiana USCM6 and method for preventing and treating pests

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Isolation and Identification of Beauveria bassiana Bb0901

[0057] The larvae of Spodoptera frugiperda and the source of Spodoptera frugiperda for testing: On September 7, 2020, the larvae of Spodoptera frugiperda in this experiment were found on cornfields in Xiaobai Township, Taigu District, Jinzhong City, Shanxi Province. The larvae of Spodoptera frugiperda were collected from cornfields in Xiaobai Township, Taigu District, Jinzhong City, Shanxi Province. They were raised on corn leaves until they pupated in the laboratory. After they emerged and laid eggs, a laboratory population was established as the source of test insects. The temperature for cultivating Spodoptera frugiperda is 26°C±1°C, the relative humidity is 60wt.%~70wt.%, and the photoperiod is 16L:8D (the light time is 16h, and the dark time is 8h), so that the larvae of Spodoptera frugiperda can be obtained. .

[0058] The medium is the formula of sporulation medium and chitin medium, wherein the formula...

Embodiment 2

[0073] Determination of Optimum Concentration of Beauveria bassiana Bb0901 Infection

[0074] Beauveria bassiana B. bassiana Bb0901 is respectively made into 1×10 with volume percent content of 1% Tween-80 4 pcs mL -1 ,, 1×10 5 pcs mL -1 , 1×10 6 pcs mL -1 , 1×10 7 pcs mL -1 , 1×10 8 pcs mL -1 For the spore suspension, use an ultra-low-volume spraying can to spray the above-mentioned different concentrations of the spore suspension onto the back of the 3rd instar larvae of Spodoptera frugiperda with similar individual length and activity, and the 3rd instar larvae of Spodoptera frugiperda sprayed with spore suspension Put a single animal into a 9mm sterile Petri dish with filter paper, place it at 26±1°C, and cultivate it in a culture laboratory with a humidity of 90wt.%, and feed it once a day with corn leaves. The feeding amount is about 30 per day. square centimeters of corn leaf. Treat 30 larvae at each concentration, repeat each concentration 3 times, continue t...

Embodiment 3

[0081] Screening of compound mutagenic strains of Beauveria bassiana and determination of mutagenic effects

[0082] The medium is sporulation medium and chitin medium; the components of the medium are the same as in Example 1.

[0083] Beauveria bassiana Bb0901 isolated and purified from Example 1 is multiplied (beauveria bassiana Bb0901 is made into 1 × 10 with the sterile water containing 1% Tween-80 by volume percentage composition 7 mL -1 For the spore suspension, take 1mL of the prepared suspension in the spore-forming medium, spread it evenly with a spreading rod, and cultivate it in a constant temperature incubator at 26±1°C, in total darkness, and with a relative humidity of 75±5wt.%. , cultivated for 10d), to obtain the multiplication bacteria liquid; take three parts of the multiplication bacteria liquid, all dubbed with a concentration of 1 × 10 7 mL -1 The spore suspension was subjected to ultraviolet mutagenesis, microwave mutagenesis and sodium nitrite mutage...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to beauveria bassiana USCM6, a fungicide, application of the beauveria bassiana USCM6 and a pest control method. The invention provides Beauveria bassiana Bb0901, and the collection number of the Beauveria bassiana Bb0901 is CGMCC (China General Microbiological Culture Collection Center) No.23232. The Beauveria bassiana Bb0901 can be used for preparing a strain of Beauveria bassiana. The strain provided by the invention can effectively prevent and treat spodoptera frugiperda, and has the advantages of environmental protection and difficult generation of resistance.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to Beauveria bassiana coccidioides, bacterial agents, applications thereof and pest control methods. Background technique [0002] Spodoptera frugiperda (Spodoptera frugiperda), belongs to Lepidoptera Noctuidae, has another name called fall armyworm, fall armyworm, Smith first discovered and named in 1797 (Zhang Runzhi. Spodoptera frugiperda (Smith) larvae[ J]. Acta Applied Entomology, 2020,57(06):1332.). Fall armyworm originated in the tropical and subtropical regions of America and is widely distributed in the American continent. The insect invaded Ghana and Nigeria in southwestern Africa through international trade in 2016, and has spread to 44 African countries south of the Sahara in 2018; In May of the same year, fall armyworm invaded Asia, and was found for the first time in the Shimoga area of ​​Karnataka, India; in December of the same year, fall armyworm was f...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/14A01N63/30
Inventor 李维宏张向博杨宁
Owner SHANXI AGRI UNIV
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