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Propagation method of arbuscular mycorrhizal fungi

A technology for arbuscular mycorrhizal fungi and seeds, which is applied in the field of proliferation of arbuscular mycorrhizal fungi, can solve the problems of unstable quality, cumbersome planting management procedures, limited production quantity of inoculants, etc. The effect of sporulation

Active Publication Date: 2022-03-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the existing mycorrhizal fungus production process still has problems such as high cost, limited production of bacterial agents, small number of spore propagules, unstable quality, and cumbersome planting management procedures. Therefore, a set of efficient and simple AM ​​fungal propagation technology has been developed. The system and method have important application value in production practice

Method used

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  • Propagation method of arbuscular mycorrhizal fungi
  • Propagation method of arbuscular mycorrhizal fungi
  • Propagation method of arbuscular mycorrhizal fungi

Examples

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Embodiment 1

[0046] 1. Method

[0047] 1. Rhizophagus intraradice multiplication

[0048] Rhizophagus intraradice was propagated with different culture substrates and host plants. There were 4 treatments in total, and each treatment was repeated 4 times, with a total of 16 pots (AB280 flower pots, each pot was filled with 2kg of culture substrate, and 20g of bacterial agent was inoculated).

[0049] Treatment A: zeolite+river sand (1:1); host plant: sorghum (20 seeds per pot, 10A plants left)+red clover (40 plants planted per pot, 20 plants left);

[0050] Treatment B: zeolite + river sand (1:1); host plant: sorghum (25 seeds per pot, 15 plants left);

[0051] Treatment C: river sand + vermiculite + perlite (2:1:1); host plant: corn (8 seedlings per pot, 4 seedlings);

[0052] Treatment D: river sand + vermiculite + peat (1:4:1); host plants: corn (8 seeds per pot, 3 seedlings) + red clover (80 seeds per pot, 40 plants).

[0053] Before sowing, the substrate used was sterilized at 121°C...

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Abstract

The invention provides a propagation method of arbuscular mycorrhizal fungi, and relates to the technical field of fungus propagation. According to the expanding propagation method of the arbuscular mycorrhizal fungi provided by the invention, a good interaction effect is generated between different culture mediums and hosts and strains, so that the infection degree and sporulation quantity of the arbuscular mycorrhizal fungi are remarkably improved, and a practical basis is provided for wide-range application of a mycorrhizal biotechnology.

Description

technical field [0001] The invention belongs to the technical field of fungal propagation, and in particular relates to a method for propagation of arbuscular mycorrhizal fungi. Background technique [0002] Arbuscular mycorrhizal (AM) fungi are a class of beneficial microorganisms widely distributed in soil and can form a symbiotic system with the roots of most higher plants. AM fungi have a series of beneficial physiological and ecological effects, which can not only enhance the host plant's ability to absorb nitrogen, phosphorus, potassium and other elements in the soil through the huge mycelial network system, but also enhance the plant's salt tolerance The ability of alkali stress, drought stress, heavy metal pollution and pests and other adversity stresses is beneficial to the growth and development of plants, and is of great significance for agricultural and forestry production and landscaping. [0003] In recent years, AM fungi have become a research hotspot as a ne...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/06A01G20/00A01G22/20A01G24/12A01G24/13A01G24/15A01G24/28
CPCA01G31/00A01G22/20A01G20/00A01G24/13A01G24/12A01G24/15A01G24/28A01G7/06
Inventor 梁斌向丹黄玉丹李琳李欢刘庆李敏武亚芬张淑彬高雪
Owner QINGDAO AGRI UNIV
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