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Microencapsulated royal jelly enzymolysis polypeptide, preparation method thereof and solid powder containing microencapsulated royal jelly enzymolysis polypeptide

A technology of microencapsulation and royal jelly, which is applied in the field of solid powder, its preparation, and microencapsulation of royal jelly enzymatic peptides, which can solve the problems of spoilage, limit the processing, storage and utilization of queen bee larvae, reduce hygroscopicity, and increase the use value , Improve the effect of biological activity

Pending Publication Date: 2022-03-18
北京中蜜科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no doubt that queen bee larvae are rich in nutrients and active ingredients, but they are easily oxidized and spoiled, which limits the processing, storage and utilization of queen bee larvae

Method used

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  • Microencapsulated royal jelly enzymolysis polypeptide, preparation method thereof and solid powder containing microencapsulated royal jelly enzymolysis polypeptide
  • Microencapsulated royal jelly enzymolysis polypeptide, preparation method thereof and solid powder containing microencapsulated royal jelly enzymolysis polypeptide
  • Microencapsulated royal jelly enzymolysis polypeptide, preparation method thereof and solid powder containing microencapsulated royal jelly enzymolysis polypeptide

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Effect test

preparation example Construction

[0047] In a first aspect, the present application provides a method for preparing a microencapsulated royal jelly enzymatically hydrolyzed polypeptide, which comprises the following steps:

[0048] (1) mixing royal jelly with water, then centrifuging to get the supernatant;

[0049] (2) adding pepsin to the supernatant obtained in step (1) for enzymolysis to obtain a primary enzymolysis solution;

[0050] (3) Adding trypsin to the primary enzymolysis solution for enzymolysis to obtain a secondary enzymolysis solution;

[0051] (4) centrifuging the secondary enzymolysis solution, and taking the supernatant; and

[0052] (5) Add embedding wall material to the supernatant obtained in step (4), grind and homogenize, and obtain the microencapsulated royal jelly enzymatic polypeptide.

[0053] In this application, the protein in royal jelly is decomposed into small molecular peptides by proteolysis technology, so that the allergic problem of royal jelly can be well solved, and the...

Embodiment 1

[0086] Adopt the following preparation method of microencapsulated royal jelly enzymatic polypeptide according to the present application to prepare microencapsulated royal jelly enzymatic polypeptide:

[0087] (1) mixing royal jelly and water with a weight ratio of 1:0.8 under ultrasonic conditions, and then centrifuging to get the supernatant;

[0088] (2) adding pepsin to the supernatant obtained in step (1) at an enzyme-substrate concentration ratio of 0.3%, and enzymatically hydrolyzing for 50 minutes at a temperature of 45° C. to obtain a primary enzymolyzate;

[0089] (3) Using a sodium hydroxide solution with a mass concentration of 5% to adjust the pH of the primary enzymolysis solution to 8, add trypsin at a temperature of 40° C. at a concentration ratio of 0.7% to the enzyme substrate, and perform enzymolysis for 40 minutes. Obtain the secondary enzymatic hydrolysis solution;

[0090] (4) centrifuging the secondary enzymolysis solution, and taking the supernatant; ...

Embodiment 2

[0093] Adopt the following preparation method of microencapsulated royal jelly enzymatic polypeptide according to the present application to prepare microencapsulated royal jelly enzymatic polypeptide:

[0094] (1) mixing royal jelly and water with a weight ratio of 1:1 under ultrasonic conditions, and then centrifuging to get the supernatant;

[0095] (2) adding pepsin to the supernatant obtained in step (1) at an enzyme-substrate concentration ratio of 0.4%, and enzymatically hydrolyzing for 40 minutes at a temperature of 40° C. to obtain a primary enzymolyzate;

[0096] (3) Using a sodium hydroxide solution with a mass concentration of 8% to adjust the pH of the primary enzymolysis solution to 8, add trypsin at a temperature of 45° C. at a concentration ratio of 0.6% to the enzyme substrate, and perform enzymolysis for 60 minutes. Obtain the secondary enzymatic hydrolysis solution;

[0097] (4) centrifuging the secondary enzymolysis solution, and taking the supernatant;

...

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Abstract

The invention relates to microencapsulated royal jelly enzymolysis polypeptide, a preparation method thereof and solid powder containing the microencapsulated royal jelly enzymolysis polypeptide. The preparation method of the microencapsulated royal jelly enzymolysis polypeptide comprises the following steps: (1) mixing royal jelly with water, and then centrifuging to take supernate; (2) adding pepsase into the supernate obtained in the step (1) for enzymolysis to obtain primary enzymatic hydrolysate; (3) trypsin is added into the primary enzymatic hydrolysate for enzymolysis, and secondary enzymatic hydrolysate is obtained; (4) centrifuging the secondary enzymatic hydrolysate, and taking supernate; and (5) adding an embedding wall material into the supernate obtained in the step (4), grinding and homogenizing. According to the preparation method, active ingredients of the royal jelly can be fully obtained, the taste of the royal jelly is improved, in addition, the royal jelly is microencapsulated, and royal jelly enzymolysis polypeptide is embedded to the maximum extent. In addition, the solid powder contains microencapsulated royal jelly enzymolysis polypeptide and queen bee larva enzymolysis polypeptide, and has good functions of relieving fatigue, enhancing physical strength and the like.

Description

Technical field: [0001] The application belongs to the field of food, medicine and health products, in particular, it relates to a microencapsulated royal jelly enzymatic polypeptide, a preparation method thereof and a solid powder containing the same. Background technique: [0002] Royal Jelly (Royal Jelly), also known as royal jelly, royal jelly, etc., is a paste-like substance secreted by the tongue gland (hypopharyngeal gland) and mandibular gland of 5-15-day-old workers. It is milky white or light yellow. The special pulpy substance used to feed the queen bee and 3-day-old larvae is the only food for the queen bee larva during the entire development period and the worker bees and drone larvae within 3 days of age. It is also the food for the queen bee for life. It is similar to mammalian milk, so it is also Called bee milk. Royal jelly contains complex bioactive substances and has high nutritional value and health care functions. The protein content in fresh royal jel...

Claims

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Application Information

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IPC IPC(8): A23L33/18
CPCA23L33/18A23L21/20A23L29/06A23L33/125A23L33/105A23L29/30A23L29/35A23P10/30A23L3/3562A23J3/04A23J3/341B01J13/02A23V2002/00A23V2200/14A23V2200/30A23V2250/55A23V2250/5112A23V2250/51088A23V2250/5114A23V2250/6412A23V2250/6418A23V2250/21
Inventor 曹珍艳高凌宇国占宝张杨胡长安冯浩然
Owner 北京中蜜科技发展有限公司
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