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Medicine for treating erectile dysfunction and application thereof

A technology of erectile dysfunction and drugs, applied in the field of biomedicine, to achieve the effect of inhibiting the fibrosis of the cavernous body of the human penis

Active Publication Date: 2022-03-22
THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, the relationship between Wnt signaling pathway and erectile dysfunction has not been reported

Method used

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  • Medicine for treating erectile dysfunction and application thereof
  • Medicine for treating erectile dysfunction and application thereof
  • Medicine for treating erectile dysfunction and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In this embodiment, human cavernous body fibroblasts are isolated and cultured in vitro, including:

[0036] Separation of human corpus cavernosum tissue, the corpus cavernosum tissue obtained by surgery, rinsed with PBS three times to remove blood, and then cut into 2mm×2mm size block tissue, using digestive enzymes (including 4mg / mL type IV collagenase, 4mg / mL collagenase Type I collagenase, 3mg / mL hyaluronidase and 1.5mg / mL trypsin) digest for 30min, then use a tissue strainer to remove the bulk tissue, centrifuge to retain the cell components, use DMEM medium with 10% fetal bovine serum Culture after resuspension.

Embodiment 2

[0038] In this embodiment, the human cavernous tissue is isolated and cultured in vitro, including:

[0039] The fresh cavernous body tissue obtained by surgery was cut into about 2mm×2mm in size, and the low-sugar DMEM medium with 10% FBS was used, and an oscillating shaker was placed in a 37°C incubator, and the tissue was placed on the shaker for culture.

Embodiment 3

[0041] In this example, the human cavernous fibroblasts cultured in Example 1 are used to test the effect of inhibitors of the Wnt signaling pathway on human cavernous fibroblasts.

[0042] The human cavernous body fibroblasts cultured in Example 1 were taken for cultivation, the culture medium was a high-glucose DMED medium containing 2% fetal bovine serum, and the culture conditions were 37° C., 5% concentration of CO 2 , when the cell growth density was 50%, ICG-001 was used for treatment at a concentration of 10 μM, and an equal amount of dimethyl sulfoxide (DMSO) was used as a control. After 14 days of treatment, the cell morphology was observed, as shown in figure 1As shown in , it can be seen that the cells changed from long spindles to thin strips, the internal refractive index was significantly increased, and the cell volume was significantly decreased ( figure 2 ), indicating that the cells gradually transitioned to non-fibrotic morphology, and the transcriptome seq...

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Abstract

The invention discloses a medicine for treating erectile dysfunction and application thereof. The medicine for treating the erectile dysfunction comprises an inhibitor of a Wnt signal channel. The invention finds that the Wnt signal channel is a key channel activated by the fibroblast for the first time, and proves that the inhibitor using the Wnt signal channel can obviously inhibit the expression of collagen in the fibroblast derived from the cavernosum so as to inhibit the fibrosis of the penis cavernosum; therefore, the inhibitor of the Wnt signal channel has the potential of serving as the medicine for treating the erectile dysfunction, and a new thought is provided for developing the medicine for treating the erectile dysfunction.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to medicines for treating erectile dysfunction and applications thereof. Background technique [0002] The pathogenesis of erectile dysfunction (ED) is mainly divided into two stages: early cavernous body dysfunction and late cavernous body structural changes. For the former, the morphological structure of the corpus cavernosum has not changed significantly, and phosphodiesterase inhibitor type 5 (PDE5i), such as sildenafil or tadalafil, can be used for effective treatment by improving the relaxation state of smooth muscle; the latter The main pathological manifestation is fibrosis of cavernous tissue, including endothelial cell injury, smooth muscle fibrosis and fibroblast proliferation. It is currently believed that transforming growth factor-β (TGFβ) is the most important upstream signal leading to this process, but At present, there is a lack of effective interventions target...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/519A61K31/428A61P15/10
CPCA61K45/00A61K31/519A61K31/428A61P15/10Y02A50/30
Inventor 赵亮宇汤育新戴英波叶昆王晋华延敏博
Owner THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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