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Construction method and application of breast cancer cell strain orthotopic transplantation model

A breast cancer cell and construction method technology, applied in the field of breast cancer cell line orthotopic transplantation model, to achieve the effect of improving the success rate, precise in situ tumor formation, and simple operation

Active Publication Date: 2022-03-25
GUANGDONG LAB ANIMALS MONITORING INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The first purpose of the present invention is to provide a method for constructing a breast cancer cell line orthotopic transplantation model. The construction method is simple to operate and adopts a method of non-invasive injection of breast cancer cells, which solves the limitations of the commonly used inoculation models on the market

Method used

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  • Construction method and application of breast cancer cell strain orthotopic transplantation model
  • Construction method and application of breast cancer cell strain orthotopic transplantation model
  • Construction method and application of breast cancer cell strain orthotopic transplantation model

Examples

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Effect test

Embodiment 1

[0063] The construction method of breast cancer cell line orthotopic transplantation model, construction method is as follows:

[0064] Cell culture: select the red fluorescent-labeled LV10N-NC stable transfection MDA-MB-231 breast cancer cell line (use LV10N-NC lentivirus to infect MDA-MB-231 breast cancer cells to construct red fluorescent-labeled MDA-MB- 231 breast cancer cell line, MDA-MB-231 breast cancer cell line with red fluorescent labeling such as figure 2 shown), in the multiple growth phase of the cells, collect and adjust the cell concentration to 5×10 6 cell / ml. When the cells are ready, place on ice.

[0065] Construction of the orthotopic transplantation model: select 6 6-week-old female NSG mice, use a respiratory anesthesia machine to anesthetize the same batch of experimental mice, and use a 1ml sterile syringe to draw 0.2ml with a density of 5×10 6 cells / ml, according to the structural characteristics of the mammary gland shown in the anatomical diagram...

Embodiment 2

[0069] The construction method of breast cancer cell line orthotopic transplantation model, construction method is as follows:

[0070] Cell culture: select the PY8119 breast cancer cell line, collect and adjust the cell concentration to 6×10 in the multiple growth phase of the cells 6 cell / ml. When the cells are ready, place on ice.

[0071] Construct the orthotopic transplantation model: select 6 C57BL / 6 female mice aged 6-8 weeks, anesthetize the same batch of experimental mice with a respiratory anesthesia machine, and draw 0.2ml with a 1ml sterile syringe at a density of 6×10 6 cells / ml, according to the structural characteristics of the mammary gland shown in the anatomical diagram of the mouse, the needle is inserted from the unilateral nipple of the fifth pair of nipples, and the direction of the subcutaneous needle is connected with the fourth pair of nipples on the side. When the needle reaches Slowly inject tumor cells into the base of the mouse's hind legs. When ...

Embodiment 3

[0075] The construction method of breast cancer cell line orthotopic transplantation model, construction method is as follows:

[0076] Cell culture: select the MDA-MB-231 breast cancer cell line, collect and adjust the cell concentration to 4×10 in the multiple growth phase of the cells 6 cell / ml. When the cells are ready, place on ice.

[0077] Construction of the orthotopic transplantation model: select 6 6-week-old female NSG mice, use a respiratory anesthesia machine to anesthetize the same batch of experimental mice, and use a 1ml sterile syringe to draw 0.2ml with a density of 4×10 6 cells / ml, according to the structural characteristics of the mammary gland shown in the anatomical diagram of the mouse, the needle is inserted from the unilateral nipple of the fifth pair of nipples, and the direction of the subcutaneous needle is connected with the fourth pair of nipples on the side. When the needle reaches Slowly inject tumor cells into the base of the mouse's hind leg...

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Abstract

The invention provides a construction method and application of a breast cancer cell strain orthotopic transplantation model, and relates to the technical field of biology. According to the construction method of the breast cancer cell strain orthotopic transplantation model, firstly, a syringe is used for inserting a needle from a single-side nipple of a fifth pair of nipples of a mouse, then a breast cancer cell solution is injected into the fourth pair of nipples on the same side to the root of a rear leg of the mouse after the needle is inserted into the skin, and the breast cancer cell strain orthotopic transplantation model is constructed. According to the invention, a special subcutaneous inoculation mode is designed according to the structural characteristics of a mouse mammary tissue anatomical map, so that the non-invasive subcutaneous injection type in-situ inoculation can be realized, the accurate in-situ tumor formation can be ensured, and the success rate of the in-situ model is improved. The construction method disclosed by the invention is simple to operate, the limitation of a common inoculation mode in the current market is solved, and greater possibility is provided for large-scale and repeatable construction of the breast cancer mouse model.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a construction method and application of an orthotopic transplantation model of breast cancer cell lines. Background technique [0002] Breast cancer is the most common malignant tumor in women, accounting for nearly a quarter of all female cancers. Its morbidity and mortality rate ranks first, and it tends to be younger. Animal models are an irreplaceable and important supporting tool for tumor research. With the deepening of research on the pathogenesis and clinical treatment of breast cancer, the construction and application of breast cancer animal models have become more and more popular. Among them, the mouse model of breast cancer can better simulate the occurrence and development of human breast cancer, and has important application value in the study of breast cancer disease mechanism and preclinical pharmacodynamic evaluation. Breast cancer mouse models can be divided into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61D7/00A61K49/00
CPCA01K67/0271A61D7/00A61K49/0008A01K2207/12A01K2227/105A01K2267/0331
Inventor 胡金芳李文德关业枝陈思羽蔡嘉怡蔡女略
Owner GUANGDONG LAB ANIMALS MONITORING INST
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