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Method for detecting GPC3 based on H-rGO-Pd NPs and Au NPs (at) rGO nanometer materials

A nanomaterial and purpose technology, applied in the field of GPC3 detection based on nanocomposite materials combined with suitable ligands, can solve the problems of expensive instruments, time-consuming technical requirements, complex operations, etc., and achieve good stability, strong conductivity, and large specific surface area Effect

Pending Publication Date: 2022-03-29
GUANGDONG UNIV OF PETROCHEMICAL TECH
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Problems solved by technology

The instruments used in these methods are expensive, complicated to operate, time-consuming and technically demanding. Therefore, it is necessary to establish a fast and easy-to-operate GPC3 detection method

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  • Method for detecting GPC3 based on H-rGO-Pd NPs and Au NPs (at) rGO nanometer materials
  • Method for detecting GPC3 based on H-rGO-Pd NPs and Au NPs (at) rGO nanometer materials
  • Method for detecting GPC3 based on H-rGO-Pd NPs and Au NPs (at) rGO nanometer materials

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Embodiment Construction

[0043] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0044] The principle of the method for detecting GPC3 based on H-rGO-Pd NPs and Au NPs@rGO nanomaterials is shown in figure 1 . H-rGO-Pd NP nanocomposites were first prepared and then used to immobilize GPC3 Apt , forming H-rGO-Pd NP-GPC3 Apt Signaling probe; Au NPs@rGO was modified on the activated screen-printed electrode (SPE) by electrodeposition technology, and GPC3 Ab Fixed on the surface of the electrode; then dropwise added GPC3 and H-rGO-Pd NPs-GPC3 Apt , due to GPC3 and GPC3 Ab It can specifically bind with the GPC3 aptamer to form a stable secondary structure, and construct the patented H-rGO-Pd NPs-GPC3 Apt / GPC3 / GPC3 Ab / Au NPs@rGO / SPE sandwich-type electrochemical aptasensor; Utilizing the efficient peroxidase-like properties of H-rGO-Pd NPs nanocomposites to catalyze H 2 o 2 with AgNO 3 Reaction, so that nano-silver pa...

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Abstract

The invention relates to a non-diagnostic method for detecting GPC3 based on H-rGO-Pd NPs and Au NPs (at) rGO nanomaterials, which comprises the following steps: modifying Au NPs (at) rGO on the surface of SPE by adopting electro-deposition, and loading GPC3Ab on the surface of Au NPs (at) rGO / SPE by virtue of adsorption; the preparation method comprises the following steps: by taking an H-rGO-Pd NPs nano material as a carrier, preparing an H-rGO-Pd NPs-GPC3Apt detection probe, and constructing a sandwich type electrochemical aptamer sensor by utilizing specific binding of GPC3, GPC3Ab and GPC3Apt; a detection signal is amplified based on the effect of catalyzing silver deposition by the H-rGO-Pd NPs nano mimetic enzyme material, scanning is performed by adopting DPV of an electrochemical workstation, peak current is recorded, and GPC3 detection is realized.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a method for detecting GPC3 based on a nanocomposite material combined with a suitable ligand. Background technique [0002] Primary hepatocellular carcinoma (HCC) is a malignant tumor that is relatively common in my country and can easily threaten people's lives. Common liver cancer markers include alpha-fetoprotein (AFP), GPC3 (glypican-3), Golgi protein 73 (GP73), etc. GPC3 detection methods mainly include radioimmunoassay, fluorescence immunoassay, enzyme-linked immunosorbent assay, chemiluminescence immunoassay, flow immunoassay, electrochemical immunosensor, piezoelectric immunosensor, etc. The invention patent with the publication number CN105717104 B relates to a method of using a membrane filtration device to separate and obtain peripheral blood of liver cancer patients whose tissue samples cannot be obtained, and then detect the expression of GPC3 in the...

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3278G01N27/48
Inventor 李桂银王博刘卫兵李羚周治德
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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