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Chemotherapy immune gel as well as preparation method and application thereof

A chemotherapy immune and gel technology, which is applied in the field of chemotherapy immune gel and its preparation, can solve the problems of large clinical side effects, etc., and achieve the effect of enhanced immunogenic tumor phenotype, good therapeutic effect, and strong systemic anti-cancer immunity

Active Publication Date: 2022-04-05
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the clinical side effects are extremely large due to the non-specific cytotoxicity induced by the tumor to normal cells

Method used

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  • Chemotherapy immune gel as well as preparation method and application thereof
  • Chemotherapy immune gel as well as preparation method and application thereof
  • Chemotherapy immune gel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Establishment and analysis of mouse model iMWA

[0039] The establishment of a mouse model iMWA accelerated tumor progression and induced immunosuppression. The establishment of the mouse model and the experimental process were as follows: BALB / c mice (6-8 weeks old) were inoculated with CT26 colorectal cancer cells (per mice were inoculated with 1x10 6 100 μL of PBS for each cell). When the tumor volume grows to about 100mm 3 Treatment was started at the time (7 days after tumor implantation), and randomized grouping and treatment were performed. A mouse model of incomplete ablation was established using a microwave therapeutic apparatus (ECO-100E, Nanjing Yigao Microwave Electric Research Institute, China). After anesthetizing CT26 tumor-bearing mice, a cold-tip MWA needle with a 1 cm active needle was placed percutaneously in the middle of the long axis of the tumor, and the power and time of ablation were controlled at 5 W and 1-1.5 min, respectively. ...

Embodiment 2

[0044] Example 2: Preparation and Identification of Chemotherapy Immunogel Drugs

[0045] In this embodiment, a chemotherapy immune gel drug is prepared, and its preparation method specifically includes the following steps:

[0046] The fibrinogen solution (50 mg / mL) was dissolved in deionized water or PBS and mixed under ultrasound for 3 minutes. The fibrinogen solution was then heated to 37°C for 30 minutes. Then thrombin (500IU / mL) was dissolved in deionized water or PBS (containing 26mg / L NaCl and 7mg / L CaCl 2 ). The drug-loaded gel was prepared by adding a predetermined amount of OX (final concentration 1 mg / mL) and IPI549 (final concentration 0.25 mg / mL) into the above-mentioned fibrinogen solution (in this step, when only OX or For IPI549, OX@Gel and IPI549@Gel for control experiments can be prepared respectively). Then the thrombin and fibrinogen solution were mixed at a volume ratio of 1:2 with a double-barreled syringe, and a fibrin gel (200 μL) was formed within...

Embodiment 3

[0052] Example 3: Study on the Treatment of Local Residual Cancer with Chemotherapy Immunogel

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Abstract

The invention discloses chemotherapy immune gel as well as a preparation method and application thereof. The chemotherapy immune gel is fibrous protein hydrogel containing oxaliplatin and IPI549. And the IPI549 is a phosphocarnosine 3-kinase gamma inhibitor. The chemotherapy immune gel disclosed by the invention can be applied to treatment of residual tumors after microwave ablation, and through blocking of a PI3K gamma-dependent immunosuppression effect and enhancement of immunogenic tumor phenotypes brought by oxaliplatin treatment, strong systemic anti-cancer immunity can be realized, distant tumors and metastasis can be inhibited, the long-term survival rate can be increased, and the chemotherapy immune gel can be applied to treatment of the residual tumors after microwave ablation. And particularly, the medicine has a better treatment effect on tumor recurrence and metastasis caused by incomplete microwave ablation (iMWA).

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a chemotherapy immune gel and its preparation method and application. Background technique [0002] Image-guided microwave ablation (MWA) technology has been included in some clinical guidelines as the first-line treatment for solid tumors. With the development of engineering technology, MWA has been continuously improved and its advantages have been continuously improved, and it has been gradually promoted in China, Europe and the United States. However, incomplete microwave ablation (iMWA) of tumors due to factors such as tumor size and location is still an urgent need. Solved clinical problems. The local recurrence rate after tumor ablation therapy has been reported to be approximately 15%. However, when percutaneous ablation is used or the tumor volume is large (diameter greater than 3 cm), the local recurrence rate after tumor treatment can be as high as 60%. More i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/555A61K9/06A61K47/42A61P35/00A61K31/519
CPCY02A50/30
Inventor 岳雯雯沈玉婷徐辉雄官昕孙丽萍
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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