Wheat stripe rust secretory protein, identification method, transgenic plant and application

Abstract

The invention belongs to the technical field of biological gene engineering, and discloses a wheat stripe rust secretory protein, an identification method, a transgenic plant and application, the nucleotide sequence of the wheat stripe rust secretory protein is SEQ ID NO.1; the amino acid sequence of the stripe rust secretory protein is SEQ ID NO. 2. A silent segment of the stripe rust secretory protein gene Spg1 is introduced into a wheat plant, a stripe rust secretory protein gene silent plant is obtained, and it is found that the disease resistance of a transgenic plant silencing the secretory protein gene is remarkably improved after stripe rust is inoculated, and the transgenic plant shows resistance to main dominant microspecies of stripe rust. The invention provides a new way in the aspect of genetic improvement of wheat rust resistance, and has a wide application prospect in the aspect of creation of novel wheat materials. The invention proves that the disease resistance character of wheat can be improved by interfering the stripe rust secretory protein gene Spg1, so that the stripe rust secretory protein gene Spg1 can be used for culturing a novel rust-resistant disease-resistant material.

Description

technical field [0001] The invention belongs to the technical field of biological genetic engineering, and in particular relates to a protein secreted by wheat stripe rust, an identification method, a transgenic plant and an application. Background technique [0002] At present, wheat stripe rust is a fungal disease caused by Puccinia striiformis f. sptritici (Pst), which seriously damages wheat. Generally, the wheat yield loss caused by stripe rust is about 5% in the year when the stripe rust occurs, and the loss caused by the pandemic year is as high as 50%-80%, and even the harvest is severe in severe cases. [0003] It can occur in the whole growth period of wheat growth. In the early stage of the disease, orange spore piles appear on the diseased leaves. With the rupture of the leaf epidermis, yellow powdery substances, namely uredia, are released, which infect wheat again with the wind, thus completing the process. Infestation cycle. [0004] Wheat stripe rust is a s...

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Problems solved by technology

[0005] Through the above analysis, the problems and defects in the prior art are: most of the prevention and treatment of wheat stripe rust is to use chemical agents directly, and the frequent use of chemical agents is easy to cause Bacteria develop resistance to antibiotics and cause environmental pollution, so it is as important to seek biological control methods to prevent and control stripe rust

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