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Vitamin K1 analysis method, kit and application thereof

A technology of vitamins and kits, applied in the direction of analysis materials, material separation, instruments, etc., to achieve the effect of reducing the cost of use

Active Publication Date: 2022-04-12
JINAN YING SHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on magnetic solid-phase extraction of vitamin K1 based on covalent organic materials for analysis and detection.

Method used

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  • Vitamin K1 analysis method, kit and application thereof
  • Vitamin K1 analysis method, kit and application thereof
  • Vitamin K1 analysis method, kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] The COOH-Fe 3 o 4 @COFs-Diphenyl, its preparation method includes:

[0029] S1. Synthesis of COOH-Fe based on solvothermal method 3 o 4 : FeCl 3 , Ammonium Acetate and Sodium Citrate are placed in the alcohol solution to disperse evenly, adopt solvothermal reaction, and obtain it after washing, separating and drying.

[0030] S2. Preparation of magnetic adsorption material based on room temperature synthesis method: COOH-Fe prepared in step S1 3 o 4 Disperse in an acetonitrile solution containing 1,3,5-tris(4-aminophenyl)benzene (TAPB), and stir to obtain COOH-Fe 3 o 4 @TAPB; will get COOH-Fe 3 o 4 Disperse @TAPB into an acetonitrile solution containing acetic acid and 2,5-divinyl-1,4-benzenedicarbaldehyde, and stir to obtain COOH-Fe 3 o 4 @COFs, to the COOH-Fe 3 o 4 Add DMF to @COFs, after the dispersion is uniform, add 2-phenylthiophenol, and pass inert gas (such as N 2 ) after being sealed and irradiated with an ultraviolet light source, centrifuged, wa...

Embodiment

[0059] A method of analyzing vitamin K1 comprising:

[0060] Magnetic adsorption material COOH-Fe 3 o 4 Synthesis of: COOH-Fe was prepared by solvothermal method in this experiment 3 o 4 . Accurately weigh 0.970g FeCl 3 ·6H 2 O, 2.150g of ammonium acetate and 0.290g of sodium citrate were added to 50mL of ethylene glycol, ultrasonically dispersed to form a uniform solution, transferred to a 100ml reactor, and reacted at 200°C for 16h, alternately washed with deionized water and ethanol three times, passed through the external Magnetic separation yielded a black precipitate. Vacuum drying at 60°C for 12h to obtain COOH-Fe 3 o 4 , for use in the next experiment.

[0061] Magnetic adsorption material (COOH-Fe 3 o 4 @COFs-Diphenyl) room temperature synthesis method: Accurately weigh 50mg COOH-Fe 3 o 4 Nanoparticles, which are dispersed in 5mL of acetonitrile in 1,3,5-tris(4-aminophenyl)benzene (TAPB, 141mg) solution and stirred at 800r / min to obtain COOH-Fe 3 o 4 @T...

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PUM

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Abstract

The invention provides a method and a kit for analyzing vitamin K1 and application of the method and the kit, and belongs to the technical field of compound detection. The prepared magnetic adsorption material based on the covalent organic framework material is used for enriching vitamin K1, the content of vitamin K1 in a to-be-detected sample (such as serum) is detected through high performance liquid chromatography-tandem mass spectrometry, the method has good detection sensitivity, accuracy and stability, meanwhile, the magnetic adsorption material can be recycled, and the cost is low. Therefore, the use cost is effectively reduced, and the practical application value is good.

Description

technical field [0001] The invention belongs to the technical field of compound detection, and in particular relates to a method for analyzing vitamin K1, a kit and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Vitamin K1, a polycyclic aromatic ketone, widely exists in natural green plants. Vitamin K1 is an essential substance for the activation of the precursors of coagulation factors II, VII, IX, and X in the body. It is clinically used in the prevention and treatment of hypothrombin, vitamin K1 deficiency, and neonatal spontaneous hemorrhage, as well as obstructive jaundice and biliary fistula. Bleeding caused by , chronic diarrhea, etc., hyp...

Claims

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Application Information

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IPC IPC(8): G01N30/08
Inventor 冯振景叶松弭兆元
Owner JINAN YING SHENG BIOTECH