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Storage method for bull paddywack elastin

A technology for elastin and beef tendon, applied in the field of protein storage, can solve the problems of elastin variability or degradation, oxidative deterioration of elastin products, affecting performance, etc.

Pending Publication Date: 2022-04-19
海南盛美诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, elastin is easily denatured or degraded, and it is difficult to preserve. The traditional preservation method is usually to preserve elastin in preservation solution or add various protective agents, and place the preservation solution containing elastin at ultra-low temperature for cryopreservation
However, people do not buy freezers with ultra-low temperature performance for storage, and most people often keep protein products at room temperature, and do not have the awareness of freezing protein products in the refrigerator, resulting in elastin products not being stable for a short time after opening. It will oxidize and deteriorate within a week, affecting its performance

Method used

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  • Storage method for bull paddywack elastin
  • Storage method for bull paddywack elastin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A storage method for bovine gluten elastin, the storage method for bovine gluten elastin mainly includes the following preparation steps:

[0024] (1) Place the elastin directly under the lamp tube of the pulsed strong light sterilization chamber to sterilize for 10 seconds to obtain the sterilized elastin;

[0025] (2) Put glycerin and sorbitan oleate with 0.1 times the mass of glycerin in a glass, stir at 400rpm for 40min, then add 0.4 times the mass of glycerin to the sterilized elastin, under the ultrasonic conditions of 40kHz and 90W , after stirring at a speed of 400rpm for 5min, add 0.03 times the mass fraction of glycerin and 50% glutaraldehyde, stir at 400rpm for 2h, centrifuge at 1000rpm for 20min, take the supernatant, and wash the upper layer with petroleum ether and isopropanol in turn Clear liquid 10 times to get elastin microspheres;

[0026] (3) Put the elastin microspheres in a sterile homogeneous bag, seal the seal and evacuate for 20 minutes, put it ...

Embodiment 2

[0035] A storage method for bovine gluten elastin, the storage method for bovine gluten elastin mainly includes the following preparation steps:

[0036] (1) Place the elastin directly under the lamp tube in the pulsed strong light sterilization chamber for 15 seconds to obtain sterilized elastin;

[0037] (2) Put glycerin and sorbitan oleate with 0.15 times the mass of glycerin in a glass, stir at 450rpm for 40min, then add 0.5 times the mass of glycerin to the sterilized elastin, under the ultrasonic conditions of 50kHz and 110W After stirring at a speed of 450rpm for 3min, add glutaraldehyde with a mass fraction of 0.04 times the mass of glycerin and 50%, stir at 450rpm for 1h, centrifuge at 1500rpm for 15min, take the supernatant, and wash the upper layer with petroleum ether and isopropanol in turn Clear liquid 15 times to get elastin microspheres;

[0038] (3) Put the elastin microspheres in a sterile homogeneous bag, seal the seal and evacuate for 30 minutes, put it in...

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Abstract

The invention discloses a storage method for bull paddywack elastin, and relates to the technical field of protein storage. The method comprises the following steps: firstly, sterilizing elastin by using intensive pulse light, and initiating protective protein; then under ultrasonic-assisted vacuum high static pressure, the elastin microspheres are prepared, so that the active center of the elastin is wrapped, the storage of the elastin is facilitated, the molecular structure of the elastin is stabilized, and the conformation change in the treatment process is prevented; and finally, freezing and differential pressure flash evaporation are combined for drying, so that the elastin microspheres form a loose porous shape, the water activity is effectively reduced, and medium and long-term storage at room temperature is realized. According to the storage method for the bull paddywack elastin, the bull paddywack elastin can be stored for a long time in a normal-temperature environment.

Description

technical field [0001] The invention relates to the technical field of protein storage, in particular to a storage method for bovine tendon elastin. Background technique [0002] Elastin is the main component of elastic fibers. Elastic fibers mainly exist in ligaments and vessel walls. Elastic fibers and collagen fibers co-exist, endowing tissues with elasticity and tensile strength. Therefore, elastin is often used as an important skin care product and health care product Loved by people. [0003] However, elastin is easily denatured or degraded, and it is difficult to preserve. The traditional preservation method is usually to preserve elastin in preservation solution or add various protective agents, and place the preservation solution containing elastin at ultra-low temperature for cryopreservation . However, people do not buy freezers with ultra-low temperature performance for storage, and most people often keep protein products at room temperature, and do not have th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/17A23L29/00A23L3/28A23L5/30A23L3/40A61K8/64A61Q19/00
CPCA23L33/17A23L29/035A23L3/28A23L5/30A23L5/32A23L3/40A61K8/64A61Q19/00A23V2002/00A61K2800/10A23V2250/542A23V2250/6406A23V2300/10A23V2300/20A23V2300/46A23V2300/48
Inventor 陈忆宾杨维
Owner 海南盛美诺生物技术有限公司