Natural microorganism and plant source composite dual adjuvant capable of efficiently inducing body fluid and cellular immunity as well as preparation method and application of natural microorganism and plant source composite dual adjuvant

Abstract

The invention belongs to the technical field of immunology, and discloses a natural microorganism and plant source composite dual adjuvant capable of efficiently inducing body fluid and cellular immunity, the natural microorganism and plant source composite dual adjuvant comprises monophosphoryl lipid A and ophiopogonin, and the preparation method comprises the following steps: S1, dissolving the monophosphoryl lipid A with Tween 80, castor oil, ethanol and the like; s2, dissolving ophiopogonin with Tween 85, hydrogenated castor oil, glycerol and the like; and S3, mixing the monophosphoryl lipid A solution and ophiopogonin, sub-packaging and sealing. The raw materials of the dual adjuvant are easy to obtain, the preparation process is simple, the cost is low, and the dual adjuvant is stable; two natural-source adjuvants with single immune response types are utilized to exert the synergistic advantages by utilizing the complementary principle of pathways, response types and target spots, so that the effects of slowly releasing a vaccination site and promoting antigen presentation are achieved, and efficient immune response reaction of Th2 type body fluid and Th1 type cells can be generated; the organism can be rejuvenated to generate efficient natural immune response, acquired immune response can be stimulated, and the safety and the applicability are high.

Description

technical field [0001] The invention relates to the technical field of immunology, relates to a vaccine adjuvant, in particular to a compound double adjuvant derived from natural microorganisms and plants which can efficiently induce humoral and cellular immunity and its application. Background technique [0002] Vaccines are autoimmune preparations made from pathogenic microorganisms (such as bacteria, rickettsia, viruses, etc.) and their metabolites, which are artificially attenuated, inactivated, or genetically modified, and used to prevent infectious diseases. If there are only antigen components in the vaccine components, the immunogenicity is often not strong enough, and injection into the human body may not cause sufficient immune response to resist the invasion of corresponding pathogens. Therefore, when applying vaccines, they are often combined with vaccine adjuvants Inject together. Vaccine adjuvants are substances that can non-specifically change or enhance the ...

AI Technical Summary

Problems solved by technology

The main purpose of this patent is to maintain systemic safety and minimize side effects at the same time. The saponins in the vaccine adjuvant are selected from QS-7, QS-18 or QS-21. Among them, the saponins of the QS series are mainly from abroad at present, and the cost Higher, and poor hemolysis, and the above-mentioned saponins are synthetic saponins, the side effects need to be considered, and there are still certain safety hazards

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