Purifying and separating medium and separating method for cerebral cortex microvessel and large vessel RNA (Ribonucleic Acid)

Abstract

The invention belongs to the technical field of biology, and particularly relates to a purification and separation medium for cerebral cortex microvessel and large vessel RNA and a separation method of the purification and separation medium. The invention provides a purification and separation medium for cerebral cortex microvessel and macrovessel RNA. The purification and separation medium comprises a first buffer solution, a second buffer solution and a third buffer solution, wherein the first buffer solution comprises 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES), an RNA enzyme inhibitor and a Hank's balanced salt solution (HBSS); the second buffer solution comprises glucan and the first buffer solution, and the molecular weight of the glucan is 65000-75000; the third buffer solution comprises animal serum and the first buffer solution. The invention provides a cerebral cortex microvascular and macrovascular RNA purification separation medium and a separation method thereof, and can effectively solve the technical problems of low purity of purified cerebral cortex blood vessels, low purity of extracted RNA and low integrity in the prior art.

Description

technical field [0001] The application belongs to the field of biotechnology, and in particular relates to the purification and separation liquid of cerebral cortex microvessel and large blood vessel RNA and the separation method thereof. Background technique [0002] With the rapid development of molecular biology, there are more and more researches on nucleic acids. And cerebral cortex vascular RNA is widely used in medical research, especially in clinical diagnosis. The extraction of high-quality total RNA from cerebral cortex blood vessel samples is the basis for downstream sequencing, qPCR, chip detection and other technologies. However, there are technical difficulties in extracting high-quality total RNA from blood vessels in the cerebral cortex, and the difficulty in preserving RNA is a major bottleneck in the study of gene expression groups. RNA is very fragile and extremely difficult to preserve. Due to the widespread presence of RNases in nature, including RNase...

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Problems solved by technology

[0004] In view of this, the present application provides a purification solution and separation method for cerebral cortex microvessel and large blood vessel RNA, which can effectively solve the problem that the purity of the purified cerebral cortex blood vessels in the prior art is not high, and the extracted RNA has low purity and low integrity. technical issues

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