Functionalized self-assembly miRNA/polypeptide composite hydrogel suitable for cartilage regeneration and preparation method thereof

A composite hydrogel and self-assembly technology, applied in the field of biomedical materials, can solve the problems of low cost performance and poor safety

Active Publication Date: 2022-04-22
方向前 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present invention can overcome the problems of insufficient bioactivity of existing materials and filling materials used for repairing cartilage damage, poor safety and low cost performance of current growth factors, and provides a composite hydrogel of miRNA and functional polypeptide, which can be used for cartilage tissue damage restorative treatment

Method used

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  • Functionalized self-assembly miRNA/polypeptide composite hydrogel suitable for cartilage regeneration and preparation method thereof
  • Functionalized self-assembly miRNA/polypeptide composite hydrogel suitable for cartilage regeneration and preparation method thereof
  • Functionalized self-assembly miRNA/polypeptide composite hydrogel suitable for cartilage regeneration and preparation method thereof

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Embodiment Construction

[0048] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The operating methods not indicated in the following examples are generally in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.

[0049] The functionalized self-assembled miRNA / polypeptide composite hydrogel suitable for cartilage regeneration in this example uses mouse miR-29b-5p and self-assembled polypeptide link recruitment factors (SKP, SKPPGTSS, amino acid sequence as shown in SEQ ID NO: 5 Shown) fragment, its preparation method comprises steps:

[0050] 1. Dissolve self-assembled polypeptide link recruitment factors (SKP, SKPPGTSS) in pure water, and obtain four sets of functional polypeptide solutions 1 after ultrasonic d...

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Abstract

The invention discloses a functional self-assembly miRNA/polypeptide composite hydrogel suitable for cartilage regeneration and a preparation method of the functional self-assembly miRNA/polypeptide composite hydrogel. The preparation method comprises the following steps: (1) preparing a functional polypeptide aqueous solution; (2) obtaining original miRNA from sample sequencing sources of aged animals and human bodies with arthritis, and further selecting phenotypes of arthritis and aging, including Sox9, Acan, Col2a1, Mmp3, Mmp13, P16INK4a, P21, Adamts4 and Adamts5, by virtue of a fluorescent quantitative PCR (Polymerase Chain Reaction) technology and western blotting, so as to determine the miRNA with the functions of resisting aging and promoting cartilage repair, and further selecting the phenotypes of the arthritis and aging, including Sox9, Acan, Col2a1, Mmp3, Mmp13, P16INK4a, P21, Adamts4 and Adamts5; (3) dissolving the miRNA with the anti-aging and cartilage repair promoting functions determined in the step (2) by using the functional polypeptide aqueous solution to obtain a composite solution; and (4) placing the composite solution in a crosslinking condition for in-situ crosslinking to obtain the functionalized self-assembled miRNA/polypeptide composite hydrogel suitable for cartilage regeneration.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a functionalized self-assembled miRNA / polypeptide composite hydrogel suitable for cartilage regeneration and a preparation method thereof. Background technique [0002] Repair of articular cartilage defects remains a great clinical challenge due to the poor intrinsic healing potential of cartilage tissue. Most patients with full-thickness articular cartilage defects may develop degenerative changes that eventually develop osteoarthritis, the leading cause of disability worldwide. The current standard of care for articular cartilage defects includes pain relief and total joint replacement; the former is limited to symptom relief and improved joint function, and the latter is associated with infection and other associated conditions. However, there are currently no effective disease-modifying therapies that can prevent or inhibit the development of osteoarthritis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/52A61L27/54
CPCA61L27/227A61L27/52A61L27/54A61L2300/258A61L2300/252A61L2300/414A61L2430/06A61L2400/12A61L2300/412C08L89/00
Inventor 方向前诸进晋杨淑慧王秀梅范顺武
Owner 方向前
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