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Seed culture medium for improving apramycin fermentation unit and application thereof

A fermentation medium, apramycin technology, applied in the direction of fermentation, microbe-based methods, microbes, etc., can solve the problem of mycelium easy to break fermentation titer, achieve important industrial value, improve easy breakage, reduce production cost effect

Pending Publication Date: 2022-04-22
SHANGHAI INST OF PHARMA IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the mycelium producing apramycin is easy to break and the fermentation titer is low in the prior art, the present invention provides a seed medium for increasing the fermentation unit of apramycin and its application

Method used

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  • Seed culture medium for improving apramycin fermentation unit and application thereof
  • Seed culture medium for improving apramycin fermentation unit and application thereof
  • Seed culture medium for improving apramycin fermentation unit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The single substitution experiment of embodiment 1 seed culture medium cornmeal

[0060] Taking the basal medium formula as the control, under the condition that nitrogen source, inorganic salt and other components remained unchanged, the complete substitution experiment of corn flour was carried out. The shake flask was fermented for 6 days, and the fermentation unit was measured by high performance liquid phase.

[0061]

[0062] The optimized formula is: 1.0% glucose, 0.5% corn starch, 1.0% lentil powder, 0.1% yeast extract, 0.3% peptone, 0.1% calcium carbonate, and the balance is water.

Embodiment 2

[0063] Embodiment 2 seed culture medium cornstarch content experiment

[0064] Taking the optimized medium formula in Example 1 above as a comparison, corn flour was replaced by corn starch, and the amount of corn starch was investigated under the condition that other components such as nitrogen source and inorganic salt remained unchanged.

[0065] The shake flask was fermented for 6 days, and the fermentation unit was measured by high performance liquid phase.

[0066]

[0067] The optimized formula is: 1.0% glucose, 2.0% corn starch, 1.0% lentil powder, 0.1% yeast extract, 0.3% peptone, 0.1% calcium carbonate, and the balance is water.

Embodiment 3

[0068] Single substitution experiment of cold bean powder in the seed medium of embodiment 3

[0069] Taking the optimized medium formula in Example 2 as a comparison, under the condition that other components such as carbon source and inorganic salt remain unchanged, hot-pressed soybean flour, medium-temperature soybean flour, soybean meal, feather meal, peanut powder, yeast extract, etc. The chilled bean powder was completely replaced, and the amount of nitrogen source replaced was 1.0%, and the shaking flask was fermented for 6 days, and the fermentation unit was measured by high performance liquid phase.

[0070]

[0071] According to the results of the above experiments, peanut powder has the best effect. The optimized formula is: 1.0% glucose, 2.0% corn starch, 1.0% peanut powder, 0.1% yeast extract, 0.3% peptone, 0.1% calcium carbonate, and the balance is water.

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Abstract

The invention discloses a seed culture medium for improving apramycin fermentation unit and application thereof. The seed culture medium comprises: (1) 3% of a carbon source, wherein the carbon source comprises glucose and corn starch; (2) 1.4-3.9% of a nitrogen source which comprises peanut powder and also comprises one or more of yeast extract, peptone, hot pressed bean flour and / or yeast powder YP200; and (3) 0.1-0.28% of inorganic salt, the inorganic salt comprises calcium carbonate, and the balance is water, and the mass volume percentage of the inorganic salt in the fermentation medium is g / 100mL. The seed culture medium can improve the phenomenon that hyphae are easy to break in the culture process, so that the shape of the hyphae becomes thick, strong and continuous, and the fermentation yield is increased from 3.02 g / L to at least 5.42 g / L.

Description

technical field [0001] The invention belongs to the technical field of industrial microbes, and relates to a seed culture medium for improving the fermentation unit of apramycin and its application. Background technique [0002] Apramycin is a broad-spectrum antibiotic widely used. During the cultivation process of Apramycin, the mycelium in shake flask seeds is easily broken into fragments, which directly affects the final fermentation yield. At present, the methods for improving apramycin are mainly the genetic engineering transformation and strain selection of strains, but the effect is not obvious. [0003] Hong Wenrong et al. (Hong Wenrong, Guo Yanghao, Meng Chun, et al. Study on the perfect fermentation process of dark mycin-producing bacteria F-211 [J]. Journal of China Pharmaceutical University, 2000, 31 (1): 61-65) through direct spore inoculation and Air agitation resulted in well-grown mycelium, which effectively increased the fermentation unit of apramycin, but ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/60C12R1/465
CPCC12N1/20C12P19/60
Inventor 李继安林惠敏郭瑞玲孟宪纬张建斌李亚军詹佳弘杨子艳赵明芳袁笑端
Owner SHANGHAI INST OF PHARMA IND
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