Method for extracting oyster peptide by integrating superstrong dynamic wave with polymerase

Abstract

The invention discloses a method for extracting oyster peptide by integrating superstrong dynamic waves with polymerase, which comprises the following steps: removing oyster shells, washing and draining, then mixing and soaking drained oysters with a NaOH aqueous solution with the concentration of 95% for 3-5 hours, removing ash and fat on the surfaces of the oysters, washing the oysters with deionized pure water, and draining; feeding the pretreated oysters into a closed boiling tank, boiling at high temperature (110 DEG C) for 3 hours, cooling to 50-55 DEG C after the oysters are boiled and damaged, and carrying out efficient hydrolysis by adopting multiple biological enzymes integrated strong dynamic waves for three times; and after 4 hours of enzymolysis, purifying and extracting by adopting a process technology of synergistically carrying out an enzymolysis process and nanofiltration membrane separation to obtain the micromolecular oyster collagen peptide with the purity of more than 90%. According to the method disclosed by the invention, purification and extraction are carried out by adopting a process technology of synergistically carrying out an enzymolysis process and nanofiltration membrane separation, so that the micromolecular oyster collagen peptide with the purity of more than 90% and the relative molecular mass of less than 1.0 kD can be obtained.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting oyster peptides with super-power wave integrated polymerase. Background technique [0002] Collagen is a natural biopolymer expressed by animal cells. As the main component of extracellular matrix, it exists in almost all tissues, accounting for about 70% of the total protein in animals. The content is higher in the epidermis, viscera, cartilage and other connective tissues. In the animal dermis, the collagen content exceeds that in other tissues. Procollagen forms collagen after removing telopeptides under the catalysis of biological enzymes. The polypeptide mixture with non-uniform molecular weight formed after thermal denaturation of collagen is called gelatin, and the polypeptide and small molecule peptide of collagen directly undergo chemical and enzymatic degradation. [0003] Oysters are one of the sources of protein peptide preparation, and how to ex...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for extracting oyster peptides with a super-power wave integrated polymerase to solve the problem that oysters are one of the sources of protein peptides proposed in the background art, and how to extract and prepare relatively high-purity peptides from oysters Protein peptides with small molecular weights are a technically difficult problem