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Method for preventing and treating bemisia tabaci of greenhouse eggplants and culture method of beauveria bassiana

A technology of Beauveria bassiana and a culturing method is applied in the field of preventing and controlling eggplant whitefly in greenhouses, and the preparation field of Beauveria bassiana amplification slurry, which can solve the problem of increased chemical dosage, unsatisfactory control effect, and chemical insecticidal effects. It is difficult for the agent to penetrate into the body and other problems, so as to achieve the effect of improving the dispersibility.

Pending Publication Date: 2022-04-29
合肥高尔生命健康科学研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Bemisia tabaci is the main pest of eggplant in greenhouses. It not only affects the yield of eggplant, but also induces soot, which reduces the quality of eggplant. In severe cases, it affects photosynthesis and even the whole plant dies.
Bemisia tabaci occurs in shed vegetables all year round. The body surface of Bemisia tabaci is densely covered with a layer of wax powder. It is difficult for chemical insecticides to penetrate into the body, and the control effect is not ideal. chemical pesticide resistance
[0003] Biopesticides control vegetable pests, although relatively safe, but affected by many factors, sometimes the control effect is low

Method used

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  • Method for preventing and treating bemisia tabaci of greenhouse eggplants and culture method of beauveria bassiana
  • Method for preventing and treating bemisia tabaci of greenhouse eggplants and culture method of beauveria bassiana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1) Select 10m 2 In the greenhouse planted with Jingjia No. 10, 5 points were selected, and 5 leaves were selected at each point, and the insect population base was investigated before spraying the pesticide;

[0060] (2) get 300g tea cake powder and be dispersed in 1L water, obtain tea cake powder aqueous dispersion; Get the Beauveria bassiana amplification material that culture example 1 obtains and dilute, be diluted to the concentration of 1.5 billion spores / gram, obtain ball Beauveria bassiana amplification liquid; the tea cake powder is obtained by crushing the cold-pressed Camellia oleifera tea cake to 2-8 μm, which contains 12.5wt% saponins, 16.5wt% crude protein, 6.8wt% crude fat, 4.2wt% minerals and 28.2wt% easily hydrolyzable polysaccharides;

[0061] (3) Add the tea cake powder water dispersion into 49L of water, then add 200mL of Beauveria bassiana amplification liquid to obtain the spraying agent;

[0062] (4) within 10 minutes, spray the spraying agent ...

Embodiment 2

[0065] (1) Select 10m 2 In the greenhouse planted with Jingjia No. 10, 5 points were selected, and 5 leaves were selected at each point, and the insect population base was investigated before spraying the pesticide;

[0066] (2) get 350g tea cake powder and be dispersed in 1L water, obtain tea cake powder aqueous dispersion; Get the Beauveria bassiana amplification material that culture example 1 obtains and dilute, be diluted to the concentration of 1.5 billion spores / gram, obtain ball Beauveria bassiana amplification liquid; the tea cake powder is obtained by pulverizing hot-pressed Camellia oleifera tea cake to 2-8 μm, which contains 11.8wt% saponins, 12.4wt% crude protein, 5.7wt% crude fat, 3.4wt% minerals and 24.1wt% easily hydrolyzable polysaccharide;

[0067] (3) The tea cake powder aqueous dispersion is added to 49L of water, and then 250mL of Beauveria bassiana amplification liquid is added to obtain the spraying agent;

[0068] (4) within 10 minutes, spray the spra...

Embodiment 3

[0071] (1) Select 10m 2 In the greenhouse planted with Jingjia No. 10, 5 points were selected, and 5 leaves were selected at each point, and the insect population base was investigated before spraying the pesticide;

[0072] (2) get 250g tea cake powder and be dispersed in 1L water, obtain tea cake powder aqueous dispersion; Get the Beauveria bassiana amplification material that culture example 1 obtains and dilute, be diluted to the concentration of 1.5 billion spores / gram, obtain ball Beauveria bassiana amplification liquid; Described tea cake powder is identical with embodiment 1;

[0073] (3) The tea cake powder aqueous dispersion is added to 49L of water, and then 150mL of Beauveria bassiana amplification liquid is added to obtain the spraying agent;

[0074] (4) within 10 minutes, spray the spraying agent in the greenhouse;

[0075] (5) 3d, 7d, and 14d after spraying the pesticide, again select 5 leaves within the 5 points selected in step (1), and count the number of ...

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Abstract

The invention relates to a method for preventing and treating bemisia tabaci of greenhouse eggplants and a culture method of beauveria bassiana. A method for preventing and treating greenhouse eggplant bemisia tabaci comprises the following steps: (1) dispersing a beauveria bassiana amplification solution and tea cake powder in water to obtain a spraying agent; in the pesticide spraying process, the adding amount of the beauveria bassiana amplification liquid is 150-250 mL / mu, and the adding amount of the tea cake powder is 250-350 g / mu; and (2) spraying the spraying agent in a greenhouse. According to the method for preventing and treating the bemisia tabaci of the greenhouse eggplants, spores generated by the beauveria bassiana germinate and invade bemisia tabaci bodies, and the bemisia tabaci is poisoned and killed. The tea cake powder contains fat, protein, tea saponin and other components, so that the dispersibility of the beauveria bassiana in water can be improved, and nutrient substances are provided for germination of the beauveria bassiana.

Description

technical field [0001] The invention belongs to the field of killing agricultural pests, and in particular relates to a method for controlling bemisia tabaci on eggplants in greenhouses and a method for preparing Beauveria bassiana amplified slurry. Background technique [0002] Bemisia tabaci is the main pest of eggplant in greenhouses. It not only affects the yield of eggplant, but also induces soot, which reduces the quality of eggplant. In severe cases, it affects photosynthesis and even the whole plant dies. Bemisia tabaci occurs in shed vegetables all year round. The body surface of Bemisia tabaci is densely covered with a layer of wax powder. It is difficult for chemical insecticides to penetrate into the body, and the control effect is not ideal. resistance to chemical pesticides. [0003] Although biological pesticides are relatively safe for controlling vegetable pests, they are sometimes less effective due to the influence of many factors. [0004] It is necessa...

Claims

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Application Information

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IPC IPC(8): A01N65/28A01N63/30A01P7/04C12N1/14C12R1/645
CPCA01N65/28A01N63/30A01N65/00C12N1/14
Inventor 张杰马磊李昌春郑芬魏敬怀汪涵奚铃玉李丹丹程勋辉徐余强
Owner 合肥高尔生命健康科学研究院有限公司