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Method for purifying sugar in broadleaf wood pulping prehydrolysate

A pre-hydrolyzate and hydrolyzate technology, applied in the biological field, can solve the problems of high sugar loss rate and low lignin removal rate, and achieve the effects of simple method, improved sugar purification efficiency and less by-products

Pending Publication Date: 2022-04-29
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of low lignin removal rate and high sugar loss rate in existing pulping prehydrolyzate purification technology, the present invention provides a method for purifying sugar in hardwood pulp prehydrolyzate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for purifying sugar in hardwood pulp prehydrolyzate, the steps are as follows:

[0035] (1) Treat the fast-growing poplar prehydrolyzate with manganese peroxidase (the prehydrolyzate is prepared by treating poplar chips with a hydrothermal method, the mass ratio of poplar chips to water is 1:6, and the pretreatment temperature is 170°C. The pretreatment time is 60min. After the reaction, the solid and liquid are separated, the liquid is the prehydrolyzed liquid, and the solid is the hydrolyzed wood chips for subsequent pulping). The reaction temperature is 30°C, the pH is 4.5, and the amount of enzyme added is 375U·L -1 , the amount of hydrogen peroxide is 6.88mM·L -1 ,Mn 2+ (MnSO 4 ) dosage is 0.25mM·L -1 , the rotating speed of the shaker was 150 rpm, and after 6 hours of reaction, the enzyme was inactivated in a boiling water bath for 5 minutes to terminate the reaction.

[0036] (2) Add 0.2wt% activated carbon to the hydrolyzate treated with manganese ...

Embodiment 2

[0040] A method for purifying sugar in hardwood pulp prehydrolyzate, the steps are as follows:

[0041](1) Manganese peroxidase is used to treat the acacia wood pre-hydrolyzate (the pre-hydrolyzate is prepared by treating the acacia wood chips with a hydrothermal method, the mass ratio of the acacia wood chips to water is 1:6, the pretreatment temperature is 170°C, and the pretreatment time 60min, solid-liquid separation after the reaction, the liquid is the pre-hydrolyzed solution, and the solid is the hydrolyzed wood chips for subsequent pulping). The reaction temperature is 30°C, the pH is 4.5, and the amount of enzyme added is 487.5U L -1 , the amount of hydrogen peroxide is 4.4mM·L -1 ,Mn 2+ (MnSO 4 ) dosage is 0.25mM·L -1 , the rotating speed of the shaker was 150 rpm, and after 5 hours of reaction, the enzyme was inactivated in a boiling water bath for 5 minutes to terminate the reaction.

[0042] (2) Add 0.4wt% activated carbon to the hydrolyzate treated with mang...

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Abstract

The invention belongs to the technical field of biology, and relates to a method for purifying sugar in broadleaf wood pulping pre-hydrolysate, which comprises the following specific steps: treating the broadleaf wood pulping pre-hydrolysate by using manganese peroxidase, further treating the pre-hydrolysate by using adsorption materials such as activated carbon and the like, and separating to obtain the purified pre-hydrolysate. According to the method, the loss of sugar in the prehydrolysis liquid is effectively reduced, the removal rate of lignin in the prehydrolysis liquid is increased, the purification of the sugar in prehydrolysis is realized, and further efficient separation and high-value utilization of the sugar are facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for purifying sugar in hardwood pulp prehydrolyzate. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Prehydrolysis is an important pretreatment method in the process of biomass classification and utilization. In the process of dissolving pulp production, the pulping raw material is pretreated by hydrothermal method to dissolve hemicellulose and lignin into the hydrolyzate, thereby improving the subsequent pulping efficiency . The main components in the hydrolyzate are hemicellulose, lignin, a small amount of glucose, and degradation by-products such as acetic...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12P19/04A61K31/715A61K31/7004A23L33/125
CPCC12P19/02C12P19/04A61K31/715A61K31/7004A23L33/125A23V2002/00A23V2200/30A23V2250/638Y02E50/10
Inventor 张凯陈嘉川杨桂花贾志欣
Owner QILU UNIV OF TECH
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